Genome‐Wide Identification and Functional Characterization of New Serotonin N‐Acetyltransferases in Soybean
Food Science & Nutrition,
Год журнала:
2025,
Номер
13(4)
Опубликована: Апрель 1, 2025
ABSTRACT
Melatonin
is
recognized
as
a
crucial
plant
hormone
due
to
its
significant
role
in
development,
growth
promotion,
enhancing
antioxidant
activity,
and
stress
resistance.
Soybean
one
of
the
most
important
crops
globally.
To
date,
three
SNAT
genes
have
been
cloned
Arabidopsis
rice;
however,
only
gene,
GmSNAT1
,
has
characterized
soybeans.
In
this
study,
members
GmGNAT
family
were
identified
through
genome‐wide
analysis.
Two
candidate
GmSNAT
(
GmSNAT3.1
GmSNAT3.2
)
selected
based
on
phylogenetic
tree
analysis
exhibited
highly
similar
protein
tertiary
structures
ZmSNAT3.
Furthermore,
functionality
these
two
was
confirmed
for
synthesizing
N‐acetylserotonin
or
melatonin
using
serotonin
5‐methoxytryptamine
substrates
vitro.
RNA‐seq
data
indicated
that
transcription
levels
downregulated
under
conditions
dehydration
salt
stress.
Additionally,
SNAT3
from
other
leguminous
plants
homology
with
genes.
This
study
elucidates
soybeans
establishes
foundation
further
exploration
into
physiological
functions
within
legume
plants.
Язык: Английский
Identification of SNAT Gene Family and Their Response to Abiotic Stress in Citrus
Yao Qian,
Mingzhou Gu,
Chengyang Song
и другие.
Horticulturae,
Год журнала:
2025,
Номер
11(4), С. 399 - 399
Опубликована: Апрель 9, 2025
Serotonin
N-acetyltransferase
(SNAT)
is
a
crucial
enzyme
in
the
melatonin
synthesis
pathway,
playing
an
essential
role
both
biosynthesis
and
plant
resistance
to
abiotic
stress.
A
bioinformatics
approach
was
employed
identify
members
of
citrus
SNAT
gene
family
analyze
their
physicochemical
properties,
structure,
conserved
domains,
phylogenetic
relationships,
promoter
cis-acting
elements.
Additionally,
tissue-specific
expression
trifoliate
orange
patterns
under
stress
conditions
were
examined.
This
study
identified
21
across
five
genomes,
distributed
over
chromosomes,
with
majority
predicted
localize
within
chloroplasts.
These
genes
characterized
by
having
between
1
8
exons,
0
7
introns,
2
5
motifs.
Phylogenetic
analysis
classified
into
four
subgroups,
demonstrating
significant
collinearity
rice.
Analysis
regions
revealed
32
elements,
those
responsive
light,
abscisic
acid,
drought
being
most
common.
Expression
indicated
tissue
specificity,
highest
levels
detected
leaves.
Quantitative
real-time
PCR
showed
that
PtrSNAT1
notably
upregulated
various
conditions,
suggesting
its
response.
Overall,
these
findings
provide
critical
insights
for
further
functional
studies
relation
responses.
Moreover,
represents
potential
target
developing
rootstocks
enhanced
Язык: Английский