Frontiers in Plant Science,
Год журнала:
2025,
Номер
16
Опубликована: Март 31, 2025
The
challenge
of
salinity
stress
significantly
impacts
global
rice
production,
especially
in
coastal
and
arid
regions
where
the
salinization
agricultural
soils
is
on
rise.
This
review
explores
complex
physiological,
biochemical,
genetic
mechanisms
contributing
to
tolerance
(Oryza
sativa
L.)
while
examining
agronomic
multidisciplinary
strategies
bolster
resilience.
Essential
adaptations
encompass
regulation
ionic
balance,
management
antioxidants,
adjustments
osmotic
pressure,
all
driven
by
genes
such
as
OsHKT1;5
transcription
factors
like
OsbZIP73.
evolution
breeding
strategies,
encompassing
traditional
methods
cutting-edge
innovations,
has
produced
remarkable
salt-tolerant
varieties
FL478
BRRI
dhan47.
advancements
this
field
are
enhanced
including
integrated
soil
management,
crop
rotation,
chemical
treatments
spermidine,
which
through
antioxidant
activity
transcriptional
mechanisms.
Case
studies
from
South
Asia,
Sub-Saharan
Africa,
Middle
East
and,
Australia
demonstrate
transformative
potential
utilizing
varieties;
however,
challenges
persist,
polygenic
nature
tolerance,
environmental
variability,
socioeconomic
barriers.
highlights
importance
collaborative
efforts
across
various
disciplines,
merging
genomic
technologies,
sophisticated
phenotyping,
inclusive
practices
foster
climate-resilient
sustainable
cultivation.
work
seeks
navigate
complexities
its
implications
for
food
security,
employing
inventive
cohesive
confront
posed
climate
change.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(23), С. 12537 - 12537
Опубликована: Ноя. 22, 2024
Salt
stress
is
a
major
abiotic
stressor
that
limits
plant
growth,
development,
and
agricultural
productivity,
especially
in
regions
with
high
soil
salinity.
With
the
increasing
salinization
of
soils
due
to
climate
change,
developing
salt-tolerant
crops
has
become
essential
for
ensuring
food
security.
This
review
consolidates
recent
advances
genetics,
transcription
factors
(TFs),
next-generation
sequencing
(NGS)
technologies
are
pivotal
enhancing
salt
tolerance
crops.
It
highlights
critical
genes
involved
ion
homeostasis,
osmotic
adjustment,
signaling
pathways,
which
contribute
resilience
under
saline
conditions.
Additionally,
specific
TF
families,
such
as
DREB,
NAC
(NAM,
ATAF,
CUC),
WRKY,
explored
their
roles
activating
salt-responsive
gene
networks.
By
leveraging
NGS
technologies-including
genome-wide
association
studies
(GWASs)
RNA
(RNA-seq)-this
provides
insights
into
complex
genetic
basis
tolerance,
identifying
novel
regulatory
networks
underpin
adaptive
responses.
Emphasizing
integration
tools,
research,
NGS,
this
presents
comprehensive
framework
accelerating
development
crops,
contributing
sustainable
agriculture
saline-prone
areas.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(6), С. 2594 - 2594
Опубликована: Март 13, 2025
Lettuce
is
a
globally
cultivated
and
consumed
leafy
crop.
Here
we
developed
an
efficient
tobacco
rattle
virus
(TRV)-based
guide
RNA
(gRNA)
delivery
system
for
CRISPR/Cas
editing
in
the
commercial
lettuce
cultivar
‘Noga’.
Plants
stably
expressing
Cas9
were
inoculated
with
TRV
vectors
carrying
gRNAs
targeting
five
nutrient-associated
genes.
The
achieved
average
efficiency
of
48.7%,
up
to
78.9%
regenerated
plantlets
showing
independent
mutations.
This
approach
eliminates
need
antibiotic
selection,
simplifying
tissue
culture
processes.
supports
diverse
applications,
including
Cas12a
large-fragment
deletions
using
dual
gRNA
sets.
Targeting
fructan
1-exohydrolase
2
(1-FEH2)
gene
produced
knockout
lines
significant
increases
prebiotic
dietary
fibre
content,
5.2-fold,
rise
degree
polymerisation
by
2.15
units
compared
controls.
Combining
1-FEH1
1-FEH2
knockouts
did
not
further
increase
levels,
revealing
as
predominant
isozyme
lettuce.
RT-qPCR
analysis
showed
reduced
expression
upstream
biosynthetic
enzyme
sucrose:sucrose
1-fructosyl
transferase
(1-SST),
suggesting
potential
feedback
inhibition
metabolism.
TRV-based
approach,
utilised
here
could
be
applied
improve
other
valuable
traits
lettuce,
may
inspire
similar
systems
enhance
nutritional
content
crops.
Food Safety and Health,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 7, 2025
ABSTRACT
You
are
what
you
eat.
Genetically
modified
(GM)
foods
transforming
modern
agriculture
by
enhancing
nutrition,
sustainability,
and
resilience
to
environmental
challenges.
This
review
highlights
the
benefits
of
GM
crops,
including
pest
resistance,
nutrient
enrichment,
roles
in
mycotoxin
reduction,
biofuel
production,
pharmaceuticals.
Although
offer
solutions
for
global
food
security,
concerns
persist
regarding
allergenicity,
cancer
risk,
reproductive
health,
gut
microbiota
disruption.
Advanced
detection
methods,
such
as
PCR‐based
assays,
immunoassays,
next‐generation
sequencing
(NGS),
pivotal
accurate
identification
regulation,
ensuring
unauthorized
modifications
excluded
from
supply.
Emerging
technologies,
CRISPR‐based
diagnostics,
promise
greater
specificity
affordability
molecular‐level
detection.
The
advocates
a
multidisciplinary
approach—integrating
genetics,
immunology,
toxicology—to
address
safety
refine
technologies.
International
regulatory
frameworks
must
balance
innovation
with
health
safeguards.
Consumer
education
is
vital
fostering
trust
acceptance
foods.
Future
developments
may
include
crops
fortified
against
malnutrition,
resilient
climate
change,
engineered
medicinal
properties.
Collaboration
among
researchers,
regulators,
public
critical
maximize
while
their
sustainability
addressing
Frontiers in Plant Science,
Год журнала:
2025,
Номер
16
Опубликована: Апрель 15, 2025
The
rhizosphere,
a
biologically
active
zone
where
plant
roots
interface
with
soil,
plays
crucial
role
in
enhancing
health,
resilience,
and
stress
tolerance.
As
key
component
achieving
Sustainable
Development
Goal
2,
the
rhizosphere
is
increasingly
recognized
for
its
potential
to
promote
sustainable
agricultural
productivity.
Engineering
microbiome
emerging
as
an
innovative
strategy
foster
growth,
improve
adaptation,
restore
soil
health
while
mitigating
detrimental
effects
of
conventional
farming
practices.
This
review
synthesizes
recent
advancements
omics
technologies,
sequencing
tools,
synthetic
microbial
communities
(SynComs),
which
have
provided
insights
into
complex
interactions
between
plants
microbes.
We
examine
root
exudates,
composed
organic
acids,
amino
sugars,
secondary
metabolites,
biochemical
cues
that
shape
beneficial
rhizosphere.
further
explores
how
advanced
techniques
like
metagenomics
metabolomics
are
employed
elucidate
mechanisms
by
exudates
influence
health.
Tailored
SynComs
shown
promising
resilience
against
both
abiotic
stresses
(e.g.,
drought
salinity)
biotic
challenges
pathogens
pests).
Integration
these
microbiomes
optimized
exudate
profiles
has
been
nutrient
cycling,
suppress
diseases,
alleviate
environmental
stresses,
thus
contributing
more
By
leveraging
multi-disciplinary
approaches
optimizing
profiles,
ecological
engineering
plant-microbiome
presents
pathway
boosting
crop
approach
also
aids
managing
soil-borne
reducing
chemical
input
dependency,
aligning
Goals
aimed
at
global
food
security
sustainability.
ongoing
research
offers
significant
promise
ensuring
long-term
productivity
preserving
future
generations.
Precision
plant
breeding
and
Crispr/cas
genome
editing
have
revolutionized
agricultural
biotechnology
opened
up
previously
unheard-of
opportunities
for
further
crop
improvement.
By
precisely
altering
genomes
through
the
use
of
cas/crispr
systems,
crops
with
beneficial
traits—like
increased
disease
resistance,
better
nutritional
value,
productivity—can
be
produced
more
quickly.
At
same
time,
improving
resilience
productivity
requires
an
understanding
how
microbes
or
plants
interact
one
another.
Plant
health
depends
on
microbial
populations,
which
influence
growth,
stress
tolerance,
resistance.
Gene
interactions
a
cooperative
relationship
that
has
potential
to
completely
transform
agriculture
by
enabling
innovative
sustainable
farming
practices.
Researchers
are
finding
new
strategies
grow
resilient,
resource-efficient,
high-performing
fusing
benefits
plant-microbe
crispr/cas
technology.
This
all-encompassing
strategy
seeks
solve
issues
posed
climate
change
growing
world
population
while
also
enhancing
food
security.
Research Square (Research Square),
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 21, 2025
Abstract
New
Breeding
Techniques
(NBTs),
such
as
CRISPR-Cas9,
TALENs,
and
ODM,
are
reshaping
the
way
we
develop
plant-based
products
for
food
cosmetic
applications
by
allowing
more
precise
efficient
trait
improvements.
However,
despite
their
scientific
potential,
global
acceptance
regulation
of
NBT-derived
continue
to
vary
widely.
This
study
provides
a
comparative
analysis
public
perceptions
across
six
countries—Spain,
France,
China,
Japan,
Brazil,
United
States—focusing
on
consumer
attitudes
toward
NBT
use
in
skincare
products.
Based
survey
data
from
724
participants,
found
clear
regional
differences
familiarity,
risk
perception,
NBTs.
Participants
China
Japan
showed
relatively
higher
awareness
openness
these
technologies.
In
contrast,
respondents
Spain
France
were
skeptical,
especially
when
it
came
environmental
risks.
opinions
evenly
split,
reflecting
ongoing
national
discussions.
Despite
U.S.
having
product-based
regulatory
framework,
trust
safety
willingness
consume
goods
was
notably
low.
One
most
consistent
findings
strong
support
mandatory
labeling
all
countries,
signaling
shared
expectation
transparency.
Claims
framing
“green”
or
sustainable
received
limited
agreement,
particularly
Western
countries.
Statistical
analyses
confirmed
significant
response
patterns,
although
average
scores
did
not
significantly.
These
results
point
importance
considering
social
cultural
context
development
communication
innovations.
While
is
gradually
increasing,
broader
adoption
will
depend
transparent
regulation,
locally
adapted
strategies,
genuine
engagement.
Building
through
clear,
evidence-based
messaging—and
respecting
values
concerns—will
be
essential
ensuring
responsible
widely
accepted
genome-edited
