Insights into the functional mechanisms of the sesquiterpene synthase GEAS and GERDS in lavender
International Journal of Biological Macromolecules,
Год журнала:
2025,
Номер
299, С. 140195 - 140195
Опубликована: Янв. 21, 2025
Язык: Английский
Functional and mechanistic insights into the stealth protein full-length CpsY is conducive to understanding immune evasion mechanisms by Mycobacterium tuberculosis
Tuberculosis,
Год журнала:
2025,
Номер
152, С. 102616 - 102616
Опубликована: Фев. 20, 2025
Язык: Английский
Impact of drought stress on biochemical and molecular responses in lavender (Lavandula angustifolia Mill.): effects on essential oil composition and antibacterial activity
Frontiers in Plant Science,
Год журнала:
2025,
Номер
16
Опубликована: Апрель 9, 2025
Drought
stress
significantly
influences
the
physiological,
biochemical,
and
molecular
processes
in
plants,
directly
impacting
their
growth
defense
mechanisms.
This
study
evaluates
response
of
Lavandula
angustifolia
(lavender)
to
different
levels
water
deficit,
with
field
capacity
(FC)
treatments
set
at
20%,
40%,
60%,
80%.
We
assessed
various
biochemical
parameters,
including
protein
content,
chlorophyll
a
b
levels,
flavonoid
phenolic
antioxidant
activity,
determine
how
drought
affects
lavender's
primary
secondary
metabolism.
As
availability
decreased,
we
observed
reduction
total
while
highest
flavonoids,
phenolics,
activity
were
recorded
control
plants
80%
FC.
Gene
expression
analysis
key
terpene
synthase
genes
revealed
differential
patterns,
linalool
α-pinene
peaking
40%
FC,
1,8-cineole
β-phellandrene
reaching
under
severe
(20%
FC).
Despite
this,
clear
correlation
between
gene
metabolite
accumulation
essential
oils
was
not
observed.
Drought-induced
changes
oil
composition
associated
enhanced
antibacterial
particularly
against
foodborne
Gram-positive
Gram-negative
bacteria,
suggesting
that
can
modulate
therapeutic
potential
lavender
oil.
Язык: Английский
Functional and Mechanistic Insights into the Fatty-Acid CoA Ligase FadK in Escherichia coli
Frontiers in Bioscience-Landmark,
Год журнала:
2025,
Номер
30(4)
Опубликована: Апрель 24, 2025
Background:
Escherichia
coli
(E.
coli)
is
a
common
opportunistic
bacterial
pathogen
in
both
human
and
animal
populations.
Fatty
acids
serve
as
the
central
carbon
energy
source,
process
mediated
by
fatty
acid-coenzyme
A
(CoA)
ligases
encoded
fad
genes
such
FadK.
However,
function
mechanism
of
FadK
remain
unclear.
Methods:
The
three-dimensional
structure
was
modeled
using
AlphaFold2.
After
expression
purification,
monomeric
successfully
isolated.
enzymatic
activity
assayed,
real-time
quantitative
polymerase
chain
reaction
(RT-qPCR)
performed
to
quantify
levels.
Results:
In
assays
acid
CoA
ligase
activity,
caprylic
found
be
optimal
substrate
for
We
determined
catalytic
conditions
FadK,
which
include
pH
7.4,
ATP
concentration
0.6
mM,
0.8
Mg2+
mM
at
37
°C.
Notably,
showed
decrease
with
increasing
concentrations
dodecyl-AMP,
further
confirmed
RT-qPCR
results.
Conclusions:
Our
findings
will
fundamental
framework
development
innovative
therapeutics
that
target
E.
infections.
Язык: Английский