Large-Scale Rice Mutant Establishment and High-Throughput Mutant Manipulation Help Advance Rice Functional Genomics DOI Creative Commons

Eyob Kassaye Wolella,

Cheng Zhen, Mengyuan Li

и другие.

Plants, Год журнала: 2025, Номер 14(10), С. 1492 - 1492

Опубликована: Май 16, 2025

Rice (Oryza sativa L.) is a stable food for over half of the world population, contributing 50-80% daily calorie intake. The completion rice genome sequencing marks significant milestone in understanding functional genomics, yet systematic identification gene functions remains bottleneck improvement. Large-scale mutant libraries which genes are lost or gained (e.g., through chemical/physical treatments, T-DNA, transposons, RNAi, CRISPR/Cas9) have proven to be powerful tools linking genotypes phenotypes. So far, using different mutagenesis approaches, million lines been established and about 5-10% predicted identified due high demands labor low-throughput utilization. DNA-barcoding-based large-scale offers unprecedented precision scalability genomics. This review summarizes loss-of-function gain-of-function library development approaches emphasizes integration DNA barcoding pooled analysis. Unique barcodes can tagged transposons/retrotransposons, constructs, miRNA/siRNA, gRNA, cDNA, allowing pooling analysis assignment that cause phenotype alterations. In addition, high-throughput phenotyping OMICS technologies accelerate functions.

Язык: Английский

Large-Scale Rice Mutant Establishment and High-Throughput Mutant Manipulation Help Advance Rice Functional Genomics DOI Creative Commons

Eyob Kassaye Wolella,

Cheng Zhen, Mengyuan Li

и другие.

Plants, Год журнала: 2025, Номер 14(10), С. 1492 - 1492

Опубликована: Май 16, 2025

Rice (Oryza sativa L.) is a stable food for over half of the world population, contributing 50-80% daily calorie intake. The completion rice genome sequencing marks significant milestone in understanding functional genomics, yet systematic identification gene functions remains bottleneck improvement. Large-scale mutant libraries which genes are lost or gained (e.g., through chemical/physical treatments, T-DNA, transposons, RNAi, CRISPR/Cas9) have proven to be powerful tools linking genotypes phenotypes. So far, using different mutagenesis approaches, million lines been established and about 5-10% predicted identified due high demands labor low-throughput utilization. DNA-barcoding-based large-scale offers unprecedented precision scalability genomics. This review summarizes loss-of-function gain-of-function library development approaches emphasizes integration DNA barcoding pooled analysis. Unique barcodes can tagged transposons/retrotransposons, constructs, miRNA/siRNA, gRNA, cDNA, allowing pooling analysis assignment that cause phenotype alterations. In addition, high-throughput phenotyping OMICS technologies accelerate functions.

Язык: Английский

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