3D Bioprinting of Cultivated Meat Followed by the Development of a Fine-Tuned YOLO Model for the Detection and Counting of Lipoblasts, Fibroblasts, and Myogenic Cells

Frontiers in Bioscience-Landmark, Год журнала: 2025, Номер 30(3)

Опубликована: Март 18, 2025

Background: Cultured meat holds significant potential as a pivotal solution for producing safe, sustainable, and high-quality protein to meet the growing demands of global population. However, scaling this technology requires innovative bioengineering approaches integrated with software methods assess growth cell cultures. This study aims develop 3D printing hybrid product subsequently design finely tuned You Only Look Once (YOLO) model detecting counting lipoblasts, fibroblasts, myogenic cells. Methods: Cultures multipotent mesenchymal stem cells (MMSCs) fibroblasts were obtained from domestic rabbit Oryctolagus cuniculus domesticus. Standard protocols employed induce adipogenic differentiation MMSCs. Fibroblasts isolated skin biopsy samples. The process utilized bioinks. engineering approach involved development unique print head into printer. Confocal transmission electron microscopy within construct was performed. A dataset digital images cells, created. Four models based on YOLOv8-seg architecture trained annotated images, implemented in Telegram bot. Results: Stable cultures obtained. 3D-printed tissue constructs composed sodium alginate, sunflower successfully fabricated. printer assembled. confirmed viability construct. Ultrastructural analysis revealed dense intercellular contacts high metabolic activity. resulting replicated organoleptic structural properties natural meat. In IT segment, single-class lipoblasts achieved metrics recall 85%, precision 77%, mean Average Precision at IoU threshold 0.50 (mAP50) 79%, which improved multiclass 92%, mAP50 81%. bot capable different types. Conclusions: achieved. Detailed microscopic demonstrated activity polymerized alginate hydrogel. engineered presents alternative Additionally, neural network models, bot, proved effective monitoring culture identifying types across three As result, we developed four YOLOv8 that outperforms model. all exhibited reduced accuracy high-density cultures, where overlapping led undercounting.

Язык: Английский

Physical stimuli-responsive 3D printable hydrogels for scalable manufacturing of bioengineered meat analogs

Trends in Food Science & Technology, Год журнала: 2025, Номер unknown, С. 104867 - 104867

Опубликована: Янв. 1, 2025

Язык: Английский

Biotechnology for sustainable agriculture: innovations in disease management

Plant Protection, Год журнала: 2024, Номер 8(4), С. 803 - 816

Опубликована: Дек. 29, 2024

The growing global population, limited natural resources, and climate change necessitate a shift toward environmentally sustainable agriculture. Traditional practices, reliant on chemical fertilizers, pesticides, poor land management, compromise food safety environmental integrity, exacerbating plant diseases weakening crop defenses. Biotechnology offers solutions by enhancing agricultural productivity while reducing pests, diseases, impacts. This review highlights the role biotechnology in agriculture, focusing biosurfactants, genetic engineering, precision biocontrol agents. Gene-editing technologies like CRISPR-Cas9 have enabled development of disease-resistant crops, improving health mitigating infections. In future, agents such as microbial inoculants plant-derived antimicrobials may replace traditional effectively managing caused bacterial, fungal, viral pathogens. present also explores potential machine learning (ML) artificial intelligence (AI) optimizing management use biosurfactants industrial applications. Biosurfactants are crucial suppressing phytopathogens, soil health, fostering beneficial plant-microbe interactions for effective disease management. Although these advancements hold great promise, further research is required to assess their long-term sustainability ecological Scaling technologies, particularly developing countries, remains significant challenge. To establish systems, an integrated approach combining genetic, environmental, technological strategies essential. study reviews emerging biotechnological solutions, emphasizing applications pathology improve resilience ensure security.

Язык: Английский