Immunomodulatory effects of Lactiplantibacillus plantarum CCFM8661 + stachyose on cyclophosphamide-induced immunosuppression mice
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Янв. 27, 2025
Introduction
The
increasing
stress
of
modern
life
has
led
to
a
decline
in
immunity,
sparking
widespread
interest
new
strategies
boost
immune
function.
Lactiplantibacillus
plantarum
and
stachyose
have
gained
attention
for
their
immune-regulating
effects,
but
the
mechanisms
combined
application
remain
unclear.
This
study
aims
investigate
immunoregulatory
effects
CCFM8661
with
cyclophosphamide-induced
immunocompromised
mice.
Methods
Mice
were
divided
into
normal
control
group,
model
group
(normal
saline),
positive
drug
(levamisole
hydrochloride,
10
mg/kg),
low,
medium,
high-dose
groups
(1.5×10
5
,
1.5×10
6
7
CFU
+
1.5
mg
stachyose).
Each
treatment
received
continuous
oral
gavage
administration
28
days.
On
days
23
24,
except
all
other
intraperitoneally
injected
cyclophosphamide
(40
mg/kg)
establish
an
immunosuppressed
model.
day
28,
30
minutes
after
final
administration,
mice
euthanized
collect
thymus,
spleen,
serum,
ileum,
feces
subsequent
analysis
indicators,
intestinal
barrier
function,
serum
cytokines,
microbiota.
Results
combination
significantly
improved
organ
atrophy,
restored
morphology,
normalized
cytokine
levels
mice,
indicating
enhanced
Additionally,
it
microbiota
diversity
by
abundance
Muribaculaceae
while
reducing
Lachnospiraceae
potentially
promoting
homeostasis.
Discussion
immune-enhancing
achieved
regulating
inflammation
maintaining
Язык: Английский
Authors' reply to letter: He who controls Clostridia and Bacteroidia controls the gut microbiome: The concept of targeted probiotics to restore the balance of keystone taxa in irritable bowel syndrome
Neurogastroenterology & Motility,
Год журнала:
2024,
Номер
36(8)
Опубликована: Май 19, 2024
Язык: Английский
The ability of the Lab4 probiotic consortium to impact upon the functionality of serum deprived human keratinocytes in vitro
Frontiers in Microbiomes,
Год журнала:
2024,
Номер
3
Опубликована: Ноя. 19, 2024
Introduction
Dysfunction
of
keratinocytes
contributes
to
a
weakened
skin
barrier
and
impaired
wound
healing
capability.
Evidence
suggests
that
probiotic
supplementation
can
lead
improved
function
in
vitro
vivo.
The
Lab4
consortium
comprises
two
strains
Lactobacillus
species
Bifidobacterium
species.
Methods
Using
serum
deprived
conditions
impair
the
functionality
immortalized
human
HaCaT
keratinocytes,
this
study
aimed
assess
impact
metabolites
derived
from
on
keratinocyte
function.
Results
A
significant
improvement
metabolic
activity
lower
apoptotic
was
observed
tandem
with
reduction
Caspase-3
gene
expression
Bax/Bcl2
ratio
following
addition
Lab4.
also
supported
integrity
which
better
maintained
increase
Filaggrin
expression.
In
damaged
enhanced
rates
re-epithelialization,
were
associated
significantly
increased
MMP-1
secretion
IL-6
IL-8.
Discussion
These
results
suggest
may
have
ability
benefit
skin.
Язык: Английский