The Role of Environmental Endocrine Disruptors on Leydig Cell Death and Senescen

The World Journal of Men s Health, Год журнала: 2025, Номер 43

Опубликована: Янв. 1, 2025

Environmental endocrine disruptors, as exogenous chemicals that interfere with hormonal behavior, are known to cause testicular Leydig cell death and senescence. The incidence of diseases the male reproductive system has been increasing over past half-century. Genetic defects alone cannot explain rapid increase in incidence, there is growing evidence environmental factors or lifestyle changes responsible for high recent years. Testicular cells occupy an important role system. In this study, we review mechanisms by which disruptors promote both senescence cells, refine former into two programmed modes, apoptosis, autophagy, further explore interactions among them, thus summarizing advances toxic effects on expecting provide a new therapeutic idea.

Язык: Английский

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Inhibitory Effect of Acetaminophen on Ocular Pigmentation and its Relationship with Thyroxine in Zebrafish Embryos

Bulletin of Environmental Contamination and Toxicology, Год журнала: 2024, Номер 112(2)

Опубликована: Фев. 1, 2024

Язык: Английский

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Capsaicin (But Not Other Vanillins) Enhances Estrogen Binding to Its Receptor: Implications for Power Sports and Cancers

Life, Год журнала: 2025, Номер 15(2), С. 208 - 208

Опубликована: Янв. 30, 2025

Capsaicin (CAP), the pain-inducing compound in chili peppers, exerts its effects mainly through transient receptor potential vanilloid channel 1 (TRPV1), which mediates pain perception and some metabolic functions. CAP has also been demonstrated to improve performance power sports (but not endurance sports) does so for females. may have anti-cancer effects. Many mechanisms explored explain these phenomena, particularly of TRPV1 activation calcium influx, glucose transporter (GLUT) upregulation inhibition insulin (INS) production, but two important ones seem missed. We demonstrate here that binds both INS estrogen (ESR1), enhancing estradiol binding. Other agonists, such as vanillin, vanillic acid acetaminophen, either no effect or inhibit Notably, TRPV1, ESR1 share significant regions homology aid identifying CAP-binding site on ESR1. Because upregulates GLUT expression thereby transport, we propose observed enhancement sports, among women, result, part, from function prevent degradation. Chronic exposure CAP, however, result downregulation internalization ESR1, well stimulation glucagon-like peptide (GLP-1) expression, downregulate starving cancer cells glucose. The binding capsaicin enhance antagonists tamoxifen, benefiting patients.

Язык: Английский

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Human sperm as an in vitro toxicity model: a versatile tool for assessing the risk of environmental contaminants

Archives of Toxicology, Год журнала: 2025, Номер unknown

Опубликована: Май 3, 2025

Abstract Contaminants of emerging concern (CECs) pose a significant threat to human and ecosystem health due their persistence, bioaccumulation in higher trophic levels, potential toxicity. While vivo models are commonly used for toxicity screening, developing alternative vitro techniques rapid environmental risk assessment is essential. Spermatozoa, with compartmentalized structure, measurable characteristics sensitivity changes, offer as an model screening. We evaluated the impact selected CECs, including pharmaceuticals pesticides, on sperm function highly motile subpopulations from donor semen. Standardised protocols were applied assess various functional parameters after 1–4 h exposure either individual or mixture chemicals. Our findings revealed that total motility insufficient detect subtle toxic effect. More responsive measures, such kinematics, induced hyperactivation, viability, mitochondrial membrane (MMP) presence reactive oxygen species (ROS) should be assessed elucidate effect environment function. Most chemicals exerted dose–response parameters, concentrations resulting most negative effects. The inherent spermatozoa oxidative stress, damage energy metabolism, makes them robust assessing These features highlight utility cellular evaluating CECs advancing methodologies.

Язык: Английский

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Long‐term opioid treatment and endocrine measures in chronic non‐cancer pain patients

European Journal of Pain, Год журнала: 2023, Номер 27(8), С. 940 - 951

Опубликована: Май 26, 2023

The prevalence of chronic non-cancer pain (CNCP) has increased dramatically the past decades, which combined with indiscriminate use prescribed opioids become a public health problem. Endocrine dysfunction may be complication long-term opioid treatment (L-TOT), but evidence is limited. This study aimed at investigating associations between L-TOT and endocrine measures in CNCP patients.Cortisol (spot after stimulation), thyrotropin (TSH), thyroxin (T4), insulin-like growth factor 1 (IGF-1), prolactin (PRL), 17-hydroxyprogesterone, androstenedione, dehydroepiandrosterone (DHEAS), sex hormone-binding globulin (SHBG), total testosterone (TT) free (fT) were measured. Group comparisons done patients controls as well on high- or low-dose morphine equivalents.Eighty-two (38 44 not receiving opioids) included. Low TT (p = 0.004) fT concentrations < 0.001), high SHBG 0.042), low DEAS 0.017) IGF-1 0.003) men found when comparing those to PRL 0.018), standard deviation score (SDS) 0.006) along lesser, normal cortisol response stimulation 0.016; p 0.012) controls. Finally, correlation levels dose was observed 0.001).Our only supports previous findings even more interestingly disclosed new associations. We recommend future studies investigate effects larger, longitudinal studies. In meanwhile, we monitoring function prescribing L-TOT.This clinical L-TOT, androgens, hormone compared results support add knowledge field, including an association levels. Compared existing research this strict inclusion/exclusion criteria, fixed time period for blood sample collection, adjustments potential confounders, been before.

Язык: Английский

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Adenomyotic Lesions Are Induced in the Mouse Uterus after Exposure to NSAID and EE2 Mixtures at Environmental Doses

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(4), С. 2003 - 2003

Опубликована: Фев. 7, 2024

The aim of this study was to assess the long-term effect exposure environmentally relevant doses non-steroidal anti-inflammatory drugs (NSAIDs; ibuprofen, and diclofenac) 17β-ethinylestradiol (EE2) on mouse uterus. NSAID-EE2 mixtures were administered in drinking water from gestational day 8 until weeks post-birth (i.e., during embryo development, lactation, puberty, sexual maturity). incidence adenomyosis lesions (presence endometrial glands inner myometrium) increased up 60% uterus 8-week-old exposed females (F1) 85% F2 (exposed father). Histological analysis revealed aberrant proliferation apoptosis, vacuolization epithelial cells, abnormal luminal glandular epithelium F1 uteri. Moreover, myofibroblast proportion (alpha-smooth muscle actin (α-SMA) expression analysis) collagen (Picrosirius red stain; a fibrosis hallmark) endometrium. Connexin-43 aberrantly distributed stroma Conversely, uterine 17β-estradiol progesterone levels not affected females. These findings demonstrated that mice, chronic NSAID EE2 at environmental intergenerationally affects physiology, particularly It may serve as model pathophysiology human adenomyosis.

Язык: Английский

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Performance evaluation of a new sponge-based moving bed biofilm reactor for the removal of pharmaceutical pollutants from real wastewater

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Июнь 20, 2024

Abstract Pharmaceutical pollutants, a group of emerging contaminants, have attracted outstanding attention in recent years, and their removal from aquatic environments has been addressed. In the current study, new sponge-based moving bed biofilm reactor (MBBR) was developed to remove chemical oxygen demand (COD) pharmaceutical compound Ibuprofen (IBU). A 30-L pilot scale MBBR constructed, which continuously fed effluent first clarifier Southern Tehran wastewater treatment plant. The controlled operational parameters were pH natural range, Dissolved Oxygen 1.5–2 mg/L, average suspended mixed liquor solids (MLSS), volatile (MLVSS) 1.68 ± 0.1 g/L 1.48 g/L, respectively. effect hydraulic retention time (HRT) (5 h, 10 15 h), filling ratio (10%, 20%, 30%), initial IBU concentration (2 5 mg/L) on efficiencies assessed. findings this study revealed COD efficiency ranging 48.9 96.7%, with best observed at an HRT 2 mg/L. Simultaneously, rate ranged 25 92.7%, highest under same ratio, albeit An extension h significantly improved both removal. However, further slightly enhanced IBU, even some cases, decreased. Based obtained results, 20% chosen as optimum state. Increasing mg/L generally removal, whereas increase caused decline This also optimized reactor’s for by using response surface methodology (RSM) independent variables HRT, concentration. regard, quadratic model found be significant. Utilizing central composite design (CCD), optimal operating 21%, 3 pinpointed, achieving efficiencies. present demonstrated that stands out promising technology

Язык: Английский

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A Single Oral Dose of Diclofenac Causes Transition of Experimental Subclinical Acute Kidney Injury to Chronic Kidney Disease

Biomedicines, Год журнала: 2022, Номер 10(5), С. 1198 - 1198

Опубликована: Май 22, 2022

Nephrotoxic drugs can cause acute kidney injury (AKI) and analgesic nephropathy. Diclofenac is potentially nephrotoxic frequently prescribed for pain control. In this study, we investigated the effects of single repetitive oral doses diclofenac in setting pre-existing subclinical AKI on further course long-term renal consequences. Unilateral ischemia–reperfusion (IRI) 15 min was performed male CD1 mice to induce AKI. Immediately after surgery, (100 mg or 200 mg) were administered. a separate experimental series, treatment with 100 over three days IRI sham surgery. Renal morphology pro-fibrotic markers 24 h two weeks dose using histology, immunofluorescence, qPCR. function studied bilateral model. A mg, but not aggravated tubular caused interstitial fibrosis atrophy later. Repetitive upregulation marker fibronectin AKI, control kidneys. conclusion, time-dependent manner already progression chronic disease (CKD)

Язык: Английский

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Cocktails of NSAIDs and 17α Ethinylestradiol at Environmentally Relevant Doses in Drinking Water Alter Puberty Onset in Mice Intergenerationally

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(6), С. 5890 - 5890

Опубликована: Март 20, 2023

Non-steroidal anti-inflammatory drugs (NSAIDs) and 17α-ethinyl-estradiol (EE2) are among the most relevant endocrine-disrupting pharmaceuticals found in environment, particularly surface drinking water due to their incomplete removal via wastewater treatment plants. Exposure of pregnant mice NSAID therapeutic doses during sex determination period has a negative impact on gonadal development fertility adults; however, effects chronic exposure at lower unknown. In this study, we investigated mixture containing ibuprofen, 2hydroxy-ibuprofen, diclofenac, EE2 two environmentally (added from fetal life until puberty) reproductive tract F1 exposed F2 offspring. animals, delayed male puberty accelerated female puberty. post-pubertal testes ovaries, differentiation/maturation different gonad cell types was altered, some these modifications were observed also non-exposed generation. Transcriptomic analysis ovaries (exposed) animals revealed significant changes gene expression profiles enriched pathways, inflammasome, metabolism extracellular matrix compared with controls (non-exposed). This suggested that drug cocktails an intergenerational impact. The identified Adverse Outcome Pathway (AOP) networks for NSAIDs EE2, everyday human exposure, will improve AOP network system concerning endocrine disruptor chemicals. It may serve identify other putative disruptors mammalian species based biomarkers.

Язык: Английский

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Maternal intake of paracetamol during pregnancy and biomarkers of male fecundity in young adult sons

Reproductive Toxicology, Год журнала: 2024, Номер 127, С. 108626 - 108626

Опубликована: Май 28, 2024

Paracetamol is suggested to have endocrine disrupting properties possibly affecting fetal programming of reproductive health that might lead impaired semen quality and changes in hormones. In this longitudinal study, we included 1058 young adult men born 1998-2000 into the Danish National Birth Cohort with follow-up at 18-21 years age. The exposure, maternal intake paracetamol, was modelled three ways: dichotomized, trimester-specific, as duration exposure categorized into: short (1-2 weeks), medium (3-9 weeks) or long (>9 vs. no intake. Outcomes characteristics, self-measured testis volume, hormone levels. We used negative binominal regression estimate percentage difference 95% confidence interval (CI) for each outcome. total, 547 (48%) sons were prenatally exposed paracetamol due least once. Maternal during pregnancy not associated any biomarkers dichotomized trimester-specific models. For mothers showed tendencies towards lower concentration (-14% [95% CI: -31%; 8%]), a higher proportion nonprogressive immotile spermatozoa (8% -4%; 21%]) DNA Fragmentation Index (16% -1%; 36%]) compared son clearly male fecundity sons. However, it cannot be ruled out characteristics.

Язык: Английский

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