Enhancing Potato Virus X Diagnosis: Chelex ™ 100 Resin RNA Extraction Method Evaluation

Journal of Experimental and Molecular Biology, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 13, 2024

Potato Virus X (PVX) is a prevalent pathogen affecting potato crops, leading to substantial yield losses. This study investigates the efficacy of Chelex™ 100 resin RNA extraction method for PVX detection, through RT-qPCR analysis. We evaluate yield, sensitivity, and detection limits this compared with Phenol-chloroform protocol. Our results demonstrate that yields superior concentrations exhibits greater sensitivity ELISA immunoassay diagnostic test. successfully detects in dilutions up 1:100 using extraction. These findings highlight potential coupled as rapid reliable detection.

Язык: Английский

Geminivirus diseases of legumes in India: current status and approaches for management

Physiology and Molecular Biology of Plants, Год журнала: 2024, Номер 31(1), С. 41 - 65

Опубликована: Ноя. 22, 2024

Язык: Английский