From Sequencing to Genome Editing in Cucurbitaceae: Application of Modern Genomic Techniques to Enhance Plant Traits
Agriculture,
Год журнала:
2024,
Номер
14(1), С. 90 - 90
Опубликована: Янв. 1, 2024
The
availability
of
genome-sequencing
and
genome-editing
techniques
has
increased
the
applicability
innovative
solutions,
opening
up
revolutionary
prospects
for
developments
in
horticultural
plant
breeding.
Cucurbitaceae
family
is
a
group
plants
great
importance
horticulture
due
to
their
high
nutritional
economic
value.
These
serve
as
important
models
elucidating
principles
development
refining
yield
improvement
strategies.
While
traditional
breeding
approaches
have
made
significant
contributions
production
cucurbits,
they
also
been
limited
by
reduced
genetic
diversity
lower
rates
variation
inherent
these
species.
This
comprehensive
review
summarises
latest
genome
editing
cucurbits.
It
covers
various
aspects
enhancing
traits
resist
biotic
stresses
such
pathogenic
fungi
viruses,
well
abiotic
adverse
climate
change,
especially
caused
drought
salinity.
study
focused
on
improvements
quality
optimisation
architecture,
sex
determination
flowers
fruit
features.
provides
insights
that
may
hold
promise
future
crop
serves
an
reference
advancement
gene-editing
technologies
Язык: Английский
From Plants to Plants: Plant-Derived Biological Polymers as Sustainable and Safe Nanocarriers for Direct Delivery of DNA to Plant Cells
Nano Letters,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 26, 2025
Sustainable
plant-derived
biopolymers
cellulose
nanocrystals
(CNC)
and
Zein
protein
were
used
to
deliver
plasmid
DNA
with
a
reporter
GFP
gene
(pDNA)
plant
cells.
CNC
modified
the
cationic
agent
2,3-epoxypropyltrimethylammonium
chloride
(EPTMAC)
electrostatically
bind
negatively
charged
pDNA.
Established
pDNA-CNC
pDNA-Zeins
conjugates
delivered
tobacco
cells
by
leaf
injection
vacuum
infiltration
of
leaves
seedlings.
Both
methods
effectively
provided
transient
expression
in
exposed
that
was
visualized
confocal
microscopy
confirmed
qRT-PCR
(GFP
expression)
Western
blot
expression).
Our
findings
support
idea
nanopolymers
derived
from
agricultural
waste
residues
can
successfully
be
advance
transformation
editing.
Delivering
genetic
material
using
biocompatible,
plant-based
large-scale
tissues
reduces
existing
limitations
increases
speed
process.
Язык: Английский
Molecular Breeding Technologies in Ornithogalum (Star of Bethlehem)
Опубликована: Янв. 1, 2025
Язык: Английский
Genetics and marker-assisted breeding for sex expression in cucumber
Frontiers in Genetics,
Год журнала:
2023,
Номер
14
Опубликована: Июнь 1, 2023
Cucumber
is
an
important
vegetable
crop
that
provides
accessible
draft
genome,
which
has
significantly
expedited
research
in
various
fields
of
molecular
genetics.
breeders
have
been
employing
methodologies
to
improve
the
yield
and
quality
crop.
These
comprise
enhancement
disease
resistance,
use
gynoecious
sex
types
their
association
with
parthenocarpy,
alterations
plant
architecture,
genetic
variability.
The
genetics
expression
are
a
complex
trait
cucumbers
but
very
significant
for
improvement
cucumber
This
review
comprises
explanation
current
status
gene(s)
involvement
its
studies,
inheritance
genes,
markers,
engineering
associated
determination,
as
well
discussion
role
ethylene
sex-determining
genes
ACS
family.
There
no
doubt
gynoecy
among
all
forms
heterosis
breeding,
if
it
fruit
can
be
enhanced
greater
extent
under
favorable
conditions.
However,
little
information
available
regard
parthenocarpy
gynoecious-type
cucumber.
sheds
light
on
mapping
could
beneficial
especially
other
scientists
working
via
traditional
assistant
approaches.
Язык: Английский
Abiotic stress responses in melon (Cucumis melo): Emerging underlying molecular mechanisms and biotechnological advances to cope with the issue
Annals of Applied Biology,
Год журнала:
2024,
Номер
185(1), С. 4 - 10
Опубликована: Июнь 10, 2024
Abstract
Melon
(
Cucumis
melo
),
a
member
of
the
Cucurbitaceae
family,
is
consumed
worldwide.
In
melon
cultivation,
abiotic
stress
has
been
found
to
negatively
affect
crop
growth
and
productivity.
Various
biotechnological
approaches
can
be
used
ameliorate
tolerance.
Previous
studies
have
investigated
effects
major
conditions
(e.g.,
drought,
salt,
cold
heat)
on
development
at
phenotypic
physiological
levels,
are
pivotal
understand
molecular
mechanisms
underlying
responses
in
melons.
This
review
aims
systematically
reviewing
current
efforts
biology
plants.
addition,
we
discuss
several
fertilization,
breeding,
genetic
engineering)
that
examined
improve
production
under
stressful
conditions.
Язык: Английский
Rapid Construction and Application of a Vector for Tobacco Ringspot Virus-Induced McPDS Silencing in Bitter Gourd
Horticulturae,
Год журнала:
2024,
Номер
10(2), С. 110 - 110
Опубликована: Янв. 23, 2024
The
aim
of
this
study
is
to
facilitate
the
construction
virus-induced
gene
silencing
vectors
and
provide
a
reference
or
positive
control
for
in
bitter
gourd.
A
recombinant
TRSV
(tobacco
ringspot
virus)
containing
two
components,
pTRSV1
pTRSV2,
was
used
study.
fragment
McPDS
target
cloned
into
pTRSV2
via
combined
enzymic
ligation
during
digestion.
components
were
agro-infiltrated
tobacco
leaves
grow
virus
particles,
which
then
extracted
mechanically
inoculated
gourd
plants.
effect
TRSV-McPDS-mediated
evaluated
by
observing
photo-bleaching
phenotype,
detecting
virus,
quantifying
downregulation
MCPDS
expression
chlorophyll
contents.
results
showed
that
all
plants
infected
with
empty
TRSV-McPDS
grew
developed
normally,
no
visible
signs
viral
disease.
However,
after
seven
days
inoculation,
only
obvious
photobleaching
leaves,
stems,
buds.
TRSV-specific
fragments
tested
out
systemically
transcription
level
dropped
84.7%.
content
also
significantly.
These
data
suggest
rapidly
constructed
VIGS
vector
successfully
induced
Taken
together,
practical
method
various
applications,
as
well
TRSV-induced
Язык: Английский
Genomic Advances in Cucurbitaceae: Implications for Crop Improvement and Breeding
International Journal of Horticulture,
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 1, 2024
The
Cucurbitaceae
family,
encompassing
a
wide
array
of
economically
and
nutritionally
significant
crops,
has
been
the
focus
extensive
genomic
research
aimed
at
enhancing
breeding
crop
improvement.Recent
advancements
in
sequencing
technologies
bioinformatics
have
led
to
genomes
from
various
species,
providing
valuable
insights
into
gene
identification,
genome
evolution,
genetic
variation.This
opened
new
avenues
for
molecular
breeding,
leveraging
transformation
editing
technologies,
including
CRISPR/Cas9,
overcome
limitations
conventional
methods.The
integration
next-generation
(NGS)
omics
approaches
furthered
our
understanding
complex
traits,
such
as
disease
resistance
fruit
quality,
facilitated
development
high-density
maps
identification
functional
genes.Additionally,
construction
cytogenetic
instrumental
revealing
structure
cucurbit
aiding
alignment
linkage
groups
with
chromosomes
marker-assisted
selection.The
exploration
diversity
through
analysis
wild
species
using
mapping
also
contributed
phylogenetic
resource
development.With
accumulation
resources
advent
high-throughput
genotyping
methods,
strategies
genome-wide
association
studies
(GWAS)
use
multi-parent
populations
emerged,
leading
discovery
quantitative
trait
loci
(QTL)
key
agronomic
traits.The
synergy
these
tools
their
implications
is
poised
revolutionize
improvement
ensuring
food
security
meeting
demands
growing
population.
Язык: Английский
Transcriptomics for Tea Plants
Chun-Fang Li,
Tao Yu,
Sa-Sa Song
и другие.
Concepts and strategies in plant sciences,
Год журнала:
2024,
Номер
unknown, С. 263 - 281
Опубликована: Янв. 1, 2024
Development of an Efficient and Reproducible in Vitro Regeneration and Transformation Protocol for Luffa cylindrica (L.) Roem
South African Journal of Botany,
Год журнала:
2024,
Номер
175, С. 115 - 121
Опубликована: Окт. 16, 2024
Язык: Английский
Effect of genetic transformation via Agrobacterium rhizogenes on the anatomical characteristics of Eruca Sativa Mill. plants
Mağallaẗ zirāʿaẗ al-rāfidayn,
Год журнала:
2023,
Номер
51(4), С. 130 - 143
Опубликована: Дек. 1, 2023
This
study
is
conducted
to
find
out
the
effect
of
genetic
transformation
by
Agrobacterium
rhizogenes
ATCC13332
on
anatomical
features
Eruca
sativa.
Stem
explants
and
leaves
were
inoculated
direct
injection
A.
rhizogenes.
The
results
indicated
high
response
E.
sativa,
as
percentage
hairy
roots
formation
was
95.3%
for
leaves.
Callus
successfully
initiated
from
both
Murashige-Skoog
(MS)
Woody
plants
(WP)
media
containing
(0.4
0.5
mg.
L-1
thidiazuron(TDZ).
Shoot
regeneration
occurred
spontaneously.
Also,
callus
originated
showed
ability
shooting.
Genetic
detected
polymerase
chain
reaction
(PCR).
Alterations
in
structure
stems
transformed
observed.
Transgenic
plants'
epidermal
cell
thickness
14.3
µm
rather
than
12
µm.
There
are
nine
vascular
bundles
regenerated
that
open
collateral
bundles.
palisade
tissue
96
µm,
while
66
non-transformed.
It
clear
led
changes
this
medicinal
plant.
Язык: Английский