The complete genome sequences of Bacillus velezensis B26: a promising biocontrol agent and biofertilizer

F1000Research, Год журнала: 2025, Номер 14, С. 170 - 170

Опубликована: Фев. 6, 2025

Bacillus velezensis is a bacterium widely recognized for its biocontrol properties and ability to promote plant growth. This study presents the whole-genome sequence of B. B26, newly identified strain isolated from chicken carcass soil in Udupi, India. The showed strong activity against fungal pathogens exhibited diverse enzymatic activities. sequencing was executed using Illumina technologies. Assembly revealed that B26 possesses genome 3,946,698-bp with G+C content 46.3%. Genome annotation 3776 protein-coding genes, 1 rRNA gene, 50 tRNA 5 ncRNA 59 pseudogenes. Functional analysis 216 genes involved carbohydrate metabolism, 3 potassium 148 linked cofactors, vitamins, prosthetic groups pigments, 10 phosphorus 24 associated iron acquisition 20 nitrogen 6 sulfur secondary 12 metabolism aromatic compounds, 43 stress response 36 virulence, disease defence. raw data generated this work have been deposited NCBI database available under accession number JAYKOV000000000. These genomic provide insight into plant-growth promoting capabilities B26.

Язык: Английский

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Genomic and Antifungal Analysis of Bacillus Velezensis Cx-H3: A Promising Biocontrol Agent for Maize Fusarium Root Rot

Опубликована: Янв. 1, 2025

Язык: Английский

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Efficiency in Aflatoxin B1 Reduction in a Solid-Based Medium by Endophytes from Stipa tenacissima L. and Their In Vitro Biocontrol and Plant Growth-Promoting Properties

Microbiology, Год журнала: 2025, Номер 94(1), С. 116 - 127

Опубликована: Фев. 1, 2025

Язык: Английский

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Optimization of the Fermentation and Preparation of the Wettable Powder Formulation of Bacillus velezensis F0b

Microorganisms, Год журнала: 2025, Номер 13(3), С. 560 - 560

Опубликована: Март 1, 2025

Bacillus velezensis is an effective biocontrol bacterium, with its microbial pesticides showing promise in biological control. In this study, we optimized the medium and conditions for fermenting strain F0b, developed a wettable powder formulation, assessed efficacy against Botrytis cinerea. We screened carriers, wetting agents, dispersants, UV protectants compatible determining optimal ratio dosage. The best F0b fermentation included rice flour (3.472%), ammonium chloride (4.898%), disodium phosphate (1.871%). ideal were 20% inoculum volume, 40 °C temperature, 80% sterile water, 72 h time, yielding viable count of 1.33 × 1010 CFU/mL. final formulation contained 54.7% dried powder, 27.3% kaolinite carrier, 16% agent (3:7 sodium dodecyl sulfate to lignin sulfonate), 2% ascorbic acid as protectant. All quality indicators met national standards, bacteria concentration 7 billion CFU/g. Field trials showed that effectively controlled cinerea, disease index significantly lower than control group. Control ranged from 50.58% 73.14% over 14 days, demonstrating commercial potential formulation.

Язык: Английский

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Integrated Management of Bacterial Wilt and Root-Knot Nematode Diseases in Pepper: Discovery of Phenazine-1-Carboxamide from Pseudomonas aeruginosa W-126

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 3335 - 3335

Опубликована: Апрель 3, 2025

Ralstonia solanacearum is an important pathogen causing bacterial wilt in pepper (Capsicum annuum L.). The concurrent infection of R. and root-knot nematodes (Meloidogyne spp.) exacerbates the severity pepper. Utilizing plant endophytic bacteria to control these mixed diseases a viable strategy. Waltheria indica L. (Sterculiaceae) traditional medicine plant. A total 209 were isolated from W. indica, Pseudomonas aeruginosa W-126 showed efficient antagonistic effect against solanacearum. Based on active compound tracking principles, was through silica gel column chromatography preparative HPLC combined with TLC analysis. It identified as phenazine-1-carboxamide (PCN) by spectral techniques (ESI-MS, 1H-NMR, 13C-NMR). PCN displayed excellent inhibitory activity solanacearum, EC50 64.16 μg/mL vitro. In addition, it certain nematocide activity, LC50 value 118.63 at 72 h. also five other phytopathogenic bacteria. structure-activity relationship indicated that phenazine skeleton acylamide groups key pharmacophores for phenazine-related compounds controlled complex M. incognita pot experiment, respective 51.41 39.80% rates. exploration secondary metabolites biocontrol can provide reference development novel pesticides.

Язык: Английский

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