Understanding the effects of mammalian sex hormones in the in vitro regeneration of Chickpea (Cicer arietinum L.) using machine learning techniques DOI Creative Commons
Kamil Haliloğlu, Aras Türkoğlu, Alireza Pour‐Aboughadareh

и другие.

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Ноя. 14, 2023

Abstract The purpose of this study was to determine the effect certain mammalian sex hormones (MSH) as an alternative synthetic in chickpea (Cicer arietinum L.) tissue culture using mathematical models. In research four different types explants, including embryo, hypocotyl, plumule and radicle, were treated with concentrations (0, 0.1, 0.001 0.00001 µM) (17β-estradiol, estrogen, progesterone testosterone). It found that MSH their had a significant on formation morphogenesis. type explant significantly affected shoot root formation, concentration formation. Based results study, it concluded would be expedient use plumula source explants culture. As result, µM showed positive effects vitro medium. XGBoost model outperformed predicting all variables based high R-squared values, low mean square error, absolute deviation. Sensitivity analysis within revealed most influential variable for number undergoing morphogenesis (40.29%) shoots–forming (33.83%), while played role determining roots–forming (47.49%). Consequently, shown have potential cultivation chickpeas. Additionally, utilization effective forecasting outcomes facilitating optimization context.

Язык: Английский

Nannochloropsis Lipids and Polyunsaturated Fatty Acids: Potential Applications and Strain Improvement DOI Creative Commons
Sofia Navalho, Narcís Ferrer-Ledo, María J. Barbosa

и другие.

Marine Drugs, Год журнала: 2025, Номер 23(3), С. 128 - 128

Опубликована: Март 15, 2025

The genus Nannochloropsis comprises a group of oleaginous microalgae that accumulate polyunsaturated fatty acids (PUFAs), especially eicosapentaenoic acid (EPA). These molecules are essential for the correct development and health humans animals. Thanks to their attractive lipid profile, is mainly marketed as feed ingredient in aquaculture. In this genus, contents cellular location PUFAs affected by growth conditions gene expression. Strain improvement through non-recombinant approaches can generate more productive strains efficient bioprocesses PUFA production. Nevertheless, lack specific markers, detection methods, selective pressure isolating such mutants remains bottleneck classical mutagenesis or quality assessment during cultivation. This review encompasses importance classes from species potential applications. Additionally, revision different ways increase content sp. using adaptive laboratory evolution also presented, well various methods label quantify lipids microalgae.

Язык: Английский

Процитировано

1
Ethyl Methanesulfonate Mutagenesis Conditions of Walnut Seeds and Preliminary Construction of Mutant Library DOI Open Access

Zhongrui Ji,

Jian Li

International Journal of Agricultural and Environmental Information Systems, Год журнала: 2025, Номер 16(1), С. 1 - 13

Опубликована: Апрель 10, 2025

This study investigates the use of ethyl methanesulfonate (EMS) mutagenesis to enhance genetic traits walnut seeds. By varying EMS concentrations, treatment times, and temperatures, optimal conditions are identified construct a diverse mutant library. Phenotypic genotypic analyses reveal mutants with superior characteristics, such as enhanced disease resistance, improved growth vigor, higher yield potential. These contribute valuable resources for improvement programs. The findings offer an effective method seed quality yield, potentially increasing farmers' income supporting sustainable agriculture. Additionally, highlights potential practical tool accelerating breeding programs generating diversity in populations.

Язык: Английский

Процитировано

0
Machine Learning Analysis of the Impact of Silver Nitrate and Silver Nanoparticles on Wheat (Triticum aestivum L.): Callus Induction, Plant Regeneration, and DNA Methylation DOI Creative Commons
Aras Türkoğlu, Kamil Haliloğlu, Fatih Demirel

и другие.

Plants, Год журнала: 2023, Номер 12(24), С. 4151 - 4151

Опубликована: Дек. 13, 2023

The objective of this study was to comprehend the efficiency wheat regeneration, callus induction, and DNA methylation through application mathematical frameworks artificial intelligence (AI)-based models. This research aimed explore impact treatments with AgNO3 Ag-NPs on various parameters. specifically concentrated analyzing RAPD profiles modeling regeneration molecular findings served as input variables in process. It included use at different concentrations (0, 2, 4, 6, 8 mg L-1). vitro epigenetic characteristics were analyzed using several machine learning (ML) methods, including support vector (SVM), random forest (RF), extreme gradient boosting (XGBoost), k-nearest neighbor classifier (KNN), Gaussian processes (GP) methods. study's results revealed that highest values for induction (CI%) embryogenic (EC%) occurred a concentration 2 L-1 Ag-NPs. Additionally, (RE) parameter reached its peak AgNO3. Taking an approach, demonstrated levels genomic template stability (GTS), 79.3%. There positive correlation seen between increased hypermethylation. Conversely, elevated associated hypomethylation. models used estimate relationships elements, treatments, concentration, GTS rates, Msp I Hpa II polymorphism, output suggested XGBoost model exhibited superior performance scores (CI), evidenced by R2 score 51.5%, which explained variances. RF 71.9% total variance showed efficacy terms EC%. Furthermore, GP model, provided most robust statistics RE, yielded value 52.5%, signifying ability account substantial portion present data. exemplifies cultivation mature embryos under influence involving

Язык: Английский

Процитировано

12
Exploring the genetic diversity and population structure of fenugreek (Trigonella foenum-graecum L.) genotypes through inter-primer binding site (iPBS)-retrotransposon marker system DOI
Kamil Haliloğlu, Hakan Özer,

Seda Melik

и другие.

Genetic Resources and Crop Evolution, Год журнала: 2024, Номер 71(7), С. 3253 - 3266

Опубликована: Янв. 9, 2024

Язык: Английский

Процитировано

4
Phenotypic and Biochemical Trait Improvement in Husk Tomatoes (Physalis sp.) through EMS-Induced Mutagenesis DOI Creative Commons
Md Ashraful Islam,

Fabeeha Tarannum,

Afsana Hossain Dina

и другие.

Horticulturae, Год журнала: 2024, Номер 10(9), С. 913 - 913

Опубликована: Авг. 27, 2024

Husk tomatoes are used in salsa and traditional medicine to alleviate illnesses. Market demand requires husk tomato varieties with improved agronomic physicochemical health benefits. Mutagen application alters plant genomes, creating new traits genetic diversity. The efficacy of EMS (ethyl methanesulfonate) was examined for morphology, bioactive compounds, phytochemical improvement. Three genotypes representing Physalis philadelphica ixocarpa underwent two hours 1.4% EMS. In addition yield yield-related properties, total phenol, antioxidant activity, chlorophyll a b levels leaves fruits, concentrations Na, Mg, Ca, Mn, Fe, Cu, K fruits were measured. Genotype-dependent morphological changes found. mutants C1T6 (85 cm) C1T7 (87.60 shorter than Control C1 (102 cm). highest 5.80 g C1T5; produced 3.08. mutant C2T6 the most (5.99 g) compared its control (2.85 g). Mutants had higher leaf/fruit chlorophyll. C1T2 activity (1.19 ng/µL). C2T1 outperformed C2 at 1.54 ng/µL phenolic content. C3T2 Ca content (1822 µg/g), while C3 861.20 µg/g. Mutations altered composition, which can be generate nutritionally superior varieties. Additionally, scientists will able study advantageous biochemical traits, enabling extensive research. Furthermore, serve as repository progression breeding procedures.

Язык: Английский

Процитировано

0
Response of Seedling Development of Clary Sage (Salvia sclarea L.) to Ethyl Methane Sulfonate (EMS) Applications DOI
Belgin ÇOŞGE ŞENKAL, Tansu USKUTOĞLU, Hülya Doğan

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Сен. 3, 2024

Abstract This study was carried out to evaluate the effects of ethyl methane sulfonate (EMS) on emergence rate and seedling characteristics clary sage (Salvia sclarea) lay foundation for future breeding studies. S. sclarea seeds were kept at 4 different EMS doses (0.25%, 0.50%, 0.75% 1%) durations (6, 12, 24 48 hours). Seedlings grown in greenhouse. 0.25% concentration determined as LD50 dose used research. Among doses, highest value (72.00%) taken from control application lowest (46.94%) 1.00 application. durations, (83.30%) gave 12hr, while (32.15%) 24hr. Considering Dose x Application Duration interaction, (88.75%) (1.25%) values ​​were recorded EMS-12hr 1.00%EMS-48hr applications, respectively. examined, ​​for root length, fresh dry weight, weight leaf length obtained The findings our showed that time increased, negatively affected, more effective than dose.

Язык: Английский

Процитировано

0
Partial unidirectional translocation from 5AL to 7BS leads to dense spike in an EMS-induced wheat mutant DOI Creative Commons

Xiaoyu Zhang,

Yongfa Wang, Yongming Chen

и другие.

BMC Genomics, Год журнала: 2024, Номер 25(1)

Опубликована: Ноя. 12, 2024

As the inflorescence of wheat, spike architecture largely determines grain productivity. Dissecting genetic basis for morphology wheat can contribute to designation ideal improve production. The present study characterizes a dense spike1 (ds1) mutant, derived from Nongda3753, induced by EMS treatment, which exhibits and reduced plant height. Through bulked segregant analysis sequencing (BSA-Seq) two segregating populations, ds1 was mapped short arm chromosome 7B. Further genotypic phenotypic analyses residual heterozygous lines F3 F6 Yong3002×ds1 revealed that there 0-135 Mb deletion in 7B associated with phenotype. reads count bulks BSA-Seq, along cytological ds1, ND3753, NIL-ds1 NIL-Y3002, confirmed partial unidirectional translocation 5AL (543–713 Mb) 7BS (0-135 exists ds1. This led an increase both copy number expression Q gene, is one reasons phenotype observed Partial identified EMS-induced mutant research illustrates effect structure variation on morphology, provides new materials several variations future breeding.

Язык: Английский

Процитировано

0
Molecular Characterisation of Some Corn (Zea mays L.) Genotypes Growing in Iğdır Province DOI Open Access
Gizem Çevik, Barış Eren

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Год журнала: 2024, Номер 14(4), С. 1708 - 1718

Опубликована: Окт. 2, 2024

Inter-primer binding site markers based on retrotransposons or iPBS retrotransposon have been useful in determining genetic diversity a large number of organisms. The CTAB technique was employed to isolate DNA, and molecular were conduct studies. Based the analyses' findings, genotypes exhibited significant degree diversity, with 100% polymorphic locus percentage. A total 154 bands generated as consequence identification experiments conducted 12 IPBS markers. typical polymorphism rate determined be 100%. Additionally, average (PIC) value, which quantifies gene all examined study, 0.228, H value 0.274. 1 14 lowest similarity ratio, coefficient 0.1600, when DICE coefficients compared. These results derived by comparing samples. analysis that greatest between 4 3 0.6747. Maize are classified into four distinct subpopulations. Genotypes can developed for reproductive research order demonstrate population. It appropriate instruments phylogenetic analyses maize varieties. This conclusion attained aforementioned. data is gathered will serve scientific foundation valuable contribution field genetics future.

Язык: Английский

Процитировано

0
Tuz Stresi Altındaki Kişniş Bitkisinin (Coriandrum sativum) DNA Metilasyon Modlarının RAPD Markırları ile Belirlenmesi DOI
Emine Uygur Göçer

Journal of Agriculture, Год журнала: 2024, Номер 7(1), С. 1 - 9

Опубликована: Янв. 10, 2024

Epigenetik, gen ifadesi ve fonksiyonundaki kalıtsal değişiklikleri tanımlayan bir alanı ifade eder, ancak DNA baz değişimi olmaksızın gerçekleşir. Bitkilerde en çok incelenen epigenetik mekanizmalar, metilasyonu histon proteinlerindeki kimyasal modifikasyonlardır. Epigenetik araştırmalarda, düşük yüksek işlem hacimli metilasyon tespiti için çeşitli teknikler kullanılmaktadır. Düşük tekniklerden biri, enzim tabanlı sitozin metilasyonunu belirleme yaklaşımıdır. Bu yöntem, biyolojik aktiviteleri terapötik potansiyeli olan tuz uygulaması yapılan kişniş (Coriandrum sativum) fidelerinde rastgele genlerdeki incelemek amacıyla çalışma, bitki genotiplerindeki anlamamıza bu değişikliklerin etkilerini değerlendirmemize olanak tanır. çalışmada stresine maruz kalan fidelerinden 12. ve24. Saatlerde alınan doku örnekleri arasındaki polimorfizmini ortaya koymak touch-down polimeraz zincir reaksiyonları duyarlı-rastgele arttırılmış polimorfik (TD-MS RAPD) tekniği uygulanmıştır. Kişniş (Cemre) genomik izole edilmiş izolatı touch- metilasyona duyarsız MspI restriksiyon enzimi duyarlı HpaII ile kesilmiştir. Kullanılan 9 oligonükleotid primerinden üç primer (OPA 05-OPD 01-OPH 10) uygulamalar saatlik örnekler arasında polimorfizmleri belirlenmiştir. TD-MS-RAPD-PZR metodu, basit temel cihazlar gerektiren ekonomik yaklaşımdır. standart termal döngü cihazı jel elektroforezi sistemi kullanılarak kolayca uygulanabilir. metotla belirlenen polimorfizmi arsında oldukça düşüktür. İki farklı (100mM-200mM) düzeyde polimorfizm olduğu sonucuna varılmıştır. Saatlik elde edilen örneklerindeki bakıldığında ise 12’nci 24’üncü saatlerde farkı tespit edilmemiştir.

Процитировано

0
Exploring the genetic diversity and population structure of origanum (Origanum rotundifolium L.) genotypes through SCoT marker system DOI Creative Commons
Halit Karagöz

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 26, 2024

Abstract Origanum rotundifolium L. is a type of origanum that has been used in folk medicine since ancient times due to its immune system strengthening effect thanks the antioxidant enzymes it contains. This study was carried out determine molecular and morphological variation rotundifolium. According results this study, findings reveal considerable among genotypes terms FHY, DHY, DLY, LSR, NB, PH, CD, CC, EOR parameters. It determined G1, G2, G3 G19 had superior properties compared other genotypes. also explores genetic diversity 50 using start codon targeted (SCoT) polymorphism markers. Of 25 SCoT primers tested, 10 successfully produced total 382 distinct scorable bands. The number bands generated per primer ranged from 21 50, with an average 38.2 primer. individual found be 7.64. mean information content (PIC) value across 0.448. Additionally, effective alleles (ne), gene (h), Shannon’s index (I) were 1.5128, 0.3333, 0.5135, respectively. Cluster analysis conducted Dice's distance unweighted pair group method arithmetic (UPGMA), which grouped into two main clusters. Genetic structure further divided five subpopulations, expected heterozygosity 0.3369. population differentiation (Fst) 0.0367 0.1281, 0.0839. first investigate germplasm SCoT-retrotransposon marker system. highlight effectiveness markers accurately assessing genotypes, offering essential resource for future breeding conservation strategies.

Язык: Английский

Процитировано

0