Plant Protection,
Год журнала:
2024,
Номер
8(4), С. 607 - 619
Опубликована: Дек. 29, 2024
The
present
study
examined
the
cultural
significance
of
wheat
and
threat
stripe
rust
(Puccinia
striiformis
f.
sp.
tritici)
in
Rawalpindi,
Pakistan,
aiming
to
protect
farming
heritage,
promote
sustainable
practices,
ensure
future
food
security.
A
mixed-methods
approach
was
employed
capture
multifaceted
role
within
Rawalpindi’s
social
structure,
traditions,
security
dynamics.
Surveys
interviews
revealed
that
most
fields
were
at
tillering
heading
stages
during
survey
period.
Sporadic
disease
outbreaks,
particularly
milking
stage,
reported
areas
such
as
Maira
Shareef
Chakri
villages,
highlighting
localized
infections.
To
achieve
objectives,
qualitative
component
involved
in-depth
with
10
agricultural
specialists
cultivation
12
experienced
farmers,
selected
through
purposive
sampling.
For
quantitative
component,
stratified
random
sampling
used
engage
300
providing
broader
insights
into
practices
awareness.
significant
contrast
emerged
regarding
awareness
disease.
Although
farmers
expressed
concern
about
its
potential
reduce
yields,
much
general
population
remained
unaware
This
underscores
importance
targeted
initiatives,
grassroots
campaigns
delivered
local
languages
address
these
knowledge
gaps
effectively.
emphasized
need
for
collaborative
efforts
among
stakeholders,
including
researchers,
policymakers,
develop
disease-resistant
varieties.
It
also
advocated
ecosystem-based
pest
management
strategies
mitigate
impact
sustainability
traditions.
Molecules,
Год журнала:
2025,
Номер
30(3), С. 586 - 586
Опубликована: Янв. 27, 2025
Endophytic
bacteria
are
an
important
source
for
developing
antimicrobial
substances.
With
the
aim
to
find
eco-friendly
agents
from
natural
sources,
Streptomyces
sp.
R6
was
isolated
Azadirachta
indica.
After
that,
a
new
spirotetronate
product,
lobophorin
S
(compound
2),
together
with
H8
1)
and
known
macrolide
compound
divergolide
C
3)
were
cultural
solution
of
strain
R6.
These
compounds
mark
first
isolation
marine-derived
microbial
products
as
lobophorins
(LOBs)
endophytic
bacteria.
The
structures
these
three
identified
by
extensive
NMR
HRMS
analyses.
activities
against
eight
fungal
four
bacterial
phytopathogens
separately
evaluated.
Compound
1
demonstrated
better
antibacterial
activity
Erwinia
carotovora,
Pseudomonas
syringae
pv.
tomato,
P.
lachrymans
MIC
values
3.91,
7.81,
15.63
μg/mL,
respectively.
Additionally,
1–3
all
showed
antifungal
Botrytis
cinerea,
1.95,
Notably,
in
vivo
effect
B.
cinerea
up
78.51
±
3.80%
at
1.95
µg/mL,
significantly
surpassing
polyoxin
B
(70.70
3.81%).
results
highlight
potential
promising
lead
development
new,
sustainable
control
plant
diseases.
Polymers,
Год журнала:
2025,
Номер
17(5), С. 692 - 692
Опубликована: Март 5, 2025
Sustainable
agriculture
increasingly
relies
on
biocontrol
agents
as
eco-friendly
solutions
to
combat
plant
diseases
while
improving
soil
health.
In
this
context,
species
of
the
genus
Bacillus,
particularly
Bacillus
subtilis,
have
shown
promise
effective
for
diseases.
This
study
demonstrates
successful
fabrication
biohybrid
materials
by
decorating
electrospun
poly(3-hydroxybutyrate)
(PHB)
fibers
with
electrosprayed
chitooligosaccharide
(COS)
and
subtilis
using
simultaneous
electrospinning
electrospraying.
During
electrospraying,
COS
formed
a
uniform
film
over
PHB
fibers,
serving
both
an
adhesive
protective
coating
that
maintained
viability
functionality
embedded
bacteria.
SEM
confirmed
bacterial
spores
were
uniformly
spread
across
COS-coated
biopolymer
fibers.
ATR-FTIR
spectroscopy
verified
deposition
mechanical
assay
demonstrated
enhancement
in
characteristics
after
formation
compared
uncoated
PHB.
addition,
improved
wetting
properties
fibrous
material,
creating
favorable
environment
growth
development.
Microbiological
tests
showed
B.
remained
viable
proliferated
normally
48
h
under
suitable
conditions
at
28
°C
agar
medium.
Furthermore,
COS/B.
subtilis-on-PHB
also
effectively
inhibited
pathogenic
fungi,
including
Alternaria
Fusarium.
These
findings
highlight
potential
dual
electrospinning/electrospraying
techniques
formulations.
The
integration
coatings
provides
promising
approach
sustainable
combining
enhanced
material
antifungal
activity.
Engineering Technology & Applied Science Research,
Год журнала:
2025,
Номер
15(2), С. 21129 - 21135
Опубликована: Апрель 3, 2025
Few-Shot
Learning
(FSL)
is
one
of
the
emerging
and
promising
approaches
used
in
machine
learning
for
image
classification
prediction.
This
work
proposes
a
Two-Way
Five-Shot
with
Meta-learning
Metric-learning
(TWFSL-MM)
model
that
can
detect
plant
diseases
limited
data,
reducing
cost
implementation
improving
quality
Azadirachta
Indica.
The
proposed
method
addresses
drawbacks
FSL
by
employing
meta-learning
metric-learning
approaches.
Experimental
results
showed
achieved
an
accuracy
92.09%,
average
loss
0.18,
precision
0.94,
recall
0.93,
F1
score
0.93.
strategy
disease
detection,
achieving
higher
dataset.
TWFSL-MM
outperforms
other
state-of-the-art
models,
demonstrating
its
potential
to
improve
crop
yields
quality.
Biophysica,
Год журнала:
2025,
Номер
5(2), С. 15 - 15
Опубликована: Апрель 22, 2025
Fungal
diseases
represent
a
significant
threat
to
global
agriculture,
leading
substantial
crop
losses
and
endangering
food
security
worldwide.
Conventional
chemical
fungicides,
while
effective,
are
increasingly
criticized
for
their
detrimental
environmental
impacts,
including
soil
degradation,
water
contamination,
the
disruption
of
non-target
organisms.
Additionally,
overuse
these
fungicides
has
accelerated
emergence
resistant
fungal
strains,
further
challenging
disease
management
strategies.
In
response
issues,
bio-nanofungicides
nano-biofungicides
have
emerged
as
cutting-edge
solution,
combining
biocompatibility,
safety,
enhanced
efficacy.
These
advanced
formulations
integrate
bio-based
agents,
such
microbial
metabolites
or
plant
extracts,
with
nanotechnology
improve
stability,
controlled
release,
targeted
delivery.
Chitosan,
silica,
silver
nanoparticles
were
extensively
studied
ability
encapsulate
bioactive
compounds
because
outstanding
antifungal
activity,
minimizing
residues.
Recent
studies
demonstrated
potential
nano-based
address
critical
gaps
in
sustainable
promising
applications
integrated
pest
systems.
Here,
we
summarize
last
advances
development
analyze
main
differences
between
them.
addition,
challenges
large-scale
production,
regulatory
approval,
comprehensive
risk
assessments
discussed.
Journal of Agricultural and Food Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 23, 2025
The
exploration
of
natural
products
as
lead
scaffolds
continues
to
serve
a
valuable
strategy
for
the
development
novel
antifungal
agents.
In
this
investigation,
we
focused
on
furanoid
compound
2-heptyl-5-hexylfuran-3-carboxylic
acid
(HHCA)
and
designed
synthesized
four
distinct
series
multisubstituted
furan
carboxylic
derivatives.
Comprehensive
structural
characterization
biological
evaluation
were
performed
all
48
compounds.
vitro
screening
against
eight
prevalent
plant
pathogens
revealed
that
some
these
derivatives
exhibited
significant
inhibitory
effects
Botrytis
cinerea
Sclerotinia
sclerotiorum.
Structure-activity
relationship
analysis
demonstrated
peptide-containing
showed
superior
potency
compared
with
their
hydrazide,
amide,
ester
counterparts.
most
promising
candidate
5g
was
further
evaluated
its
in
vivo
efficacy
two
pathogens,
S.
sclerotiorum
B.
cinerea,
indicating
potent
activity
EC50
values
17.14
μg/mL
19.63
μg/mL,
respectively.
Notably,
significantly
lower
than
those
product
HHCA
an
enhanced
potency.
These
findings
highlight
practical
potential
agent
agricultural
applications.
Mechanistic
investigations
employing
microscopic
analysis,
molecular
docking
simulations,
density
functional
theory
(DFT)
calculations
provided
insights
into
putative
mode
action
5g,
particularly
regarding
interaction
succinate
dehydrogenase
(SDH).
establish
foundation
rational
HHCA-derived
SDH-targeting
fungicidal