Environmental Technology, Год журнала: 2025, Номер unknown, С. 1 - 10
Опубликована: Май 26, 2025
Agricultural residues are frequently utilized as biomass fertilizers via compost, but their degradation is hindered by low temperatures in cold regions. Isolating strains capable of degrading cellulose at crucial for the effective recycling agricultural In this study, with ability were selected from humus soil region. Preliminary screening was performed sodium carboxymethyl culture and Congo red staining. Cellulase activity rescreened identified morphological observation, physiological biochemical tests, phylogenetic analysis Internal Transcribed Spacer sequence. We isolated a cellulose-degrading strain (LC-6) operating temperature (10°C-15°C). It Tausonia pullulans (GenBank entry number PQ227816 PQ584837). The exhibited peak activities Carboxymethyl cellulase, Cellobiohydrolase, β-Glucosidase, Filter paper enzyme 4.93, 7.62, 14.17 7.15 U/mL, respectively. After 15-days fermentation 15°C, rates hemicellulose, lignin 25.01%, 16.13% 24.35%, findings indicated that LC-6 can effectively degrade straw temperatures, demonstrating significant potential applications utilization lignocellulosic biomass.
Язык: Английский