International Journal of Science Letters,
Год журнала:
2022,
Номер
unknown
Опубликована: Июль 24, 2022
By
determining
the
species-specific
structure
by
morphological
examination
of
sperm,
it
is
possible
to
improve
some
disadvantages
related
short
and
long-term
storage
use
in
artificial
insemination
applications.
When
morphology
canary
spermatozoon
evaluated,
seen
that
has
a
long
indented
acrosome
as
well
rather
flagellum.
To
determine
semen,
methods
used
different
poultry
can
be
applied
visually
evaluated
with
shapes.
This
study
aims
provide
information
about
comparison
usability
various
staining
semen
from
songbirds.
In
this
study,
12
male
Gloster
canaries
was
collected
parameters
semen.
Collected
evaluation
methods;
Fixation
5%
formaldehyde,
Formalin
fixation
Giemsa
staining,
fixation,
SpermBlue
were
evaluated.
results
while
nucleus
more
prominent
compared
other
for
evaluation,
observed
staining.
On
hand,
when
sperm
fixed
formaldehyde
solution
indistinguishable,
changes
flagellum
determined
much
clearly.
As
result
reported
could
all
used.
Veterinary Medicine and Science,
Год журнала:
2024,
Номер
10(6)
Опубликована: Окт. 30, 2024
ABSTRACT
Sperm
cryopreservation
is
a
rather
complex
process
that
needs
to
be
adapted
wild
and
domestic
bird
species
ensure
adequate
efficiency.
This
study
aimed
determine
the
usability
of
different
cryoprotectants
in
Gloster
canary
sperm.
For
this
purpose,
sperm
samples
were
collected
from
12
2‐year‐old
male
canaries
three
times
week
using
cloacal
massage
for
4
weeks.
After
individual
evaluation,
combined.
Mixed
divided
into
two
groups
study.
Overall,
8%
dimethyl
sulfoxide
(DMSO)
ethylene
glycol
(EG)
used
as
cryoprotectants.
drawn
straws
after
adding
Dulbecco's
Modified
Eagle
Medium
(DMEM)
extender
with
high
glucose
ratio
1:1
frozen
−80°C
liquid
nitrogen
vapour
then
stored
at
−196°C.
Frozen‐thawed
semen
evaluated
regarding
motility,
vitality,
plasma
membrane
integrity
(hypoosmotic
swelling
test
[HOST]),
density
abnormal
spermatozoa
rate.
The
highest
motility
value
freezing
thawing
was
determined
EG
group
31.667%
±
4.773%.
In
addition,
normal
morphology
statistically
significantly
higher
EG‐frozen
group,
whereas
head
tail
abnormality
low
(
p
<
0.05).
DMEM
containing
more
advantageous
than
DMSO
spermatological
parameters
could
long‐term
storage
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2022,
Номер
unknown
Опубликована: Авг. 5, 2022
Abstract
Sperm
cells
are
exceptionally
morphologically
diverse
across
taxa.
However,
morphology
can
be
quite
uniform
within
species,
particularly
for
species
where
females
copulate
with
many
males
per
reproductive
bout.
Strong
sexual
selection
in
these
promiscuous
is
widely
hypothesized
to
reduce
intraspecific
sperm
variation.
Conversely,
we
hypothesize
that
size
variation
may
maintained
by
high
among-female
the
of
storage
organs,
assuming
paternity
success
improves
when
compatible
organ.
We
use
individual-based
simulations
and
an
analytical
model
evaluate
how
on
depends
promiscuity
level
organ
(hereafter,
female
preference
variation).
Simulated
showed
stabilizing
was
low,
disruptive
high,
consistent
results.
With
low
(2-3
mates
female),
all
levels
simulations,
contrasting
model.
Promiscuity
level,
or
mate
sampling,
thus
has
a
strong
impact
resulting
from
preferences.
Further,
promiscuity,
male
traits
will
occur
under
more
limited
circumstances
than
previous
models
suggest.
Variation
organs
likely
implications
highly
but
does
not
explain
differences
less
International Journal of Science Letters,
Год журнала:
2022,
Номер
unknown
Опубликована: Июль 24, 2022
By
determining
the
species-specific
structure
by
morphological
examination
of
sperm,
it
is
possible
to
improve
some
disadvantages
related
short
and
long-term
storage
use
in
artificial
insemination
applications.
When
morphology
canary
spermatozoon
evaluated,
seen
that
has
a
long
indented
acrosome
as
well
rather
flagellum.
To
determine
semen,
methods
used
different
poultry
can
be
applied
visually
evaluated
with
shapes.
This
study
aims
provide
information
about
comparison
usability
various
staining
semen
from
songbirds.
In
this
study,
12
male
Gloster
canaries
was
collected
parameters
semen.
Collected
evaluation
methods;
Fixation
5%
formaldehyde,
Formalin
fixation
Giemsa
staining,
fixation,
SpermBlue
were
evaluated.
results
while
nucleus
more
prominent
compared
other
for
evaluation,
observed
staining.
On
hand,
when
sperm
fixed
formaldehyde
solution
indistinguishable,
changes
flagellum
determined
much
clearly.
As
result
reported
could
all
used.
