Advanced sperm preservation techniques in yellow spotted mountain newts Neurergus derjugini enhance genetic management and conservation efforts DOI Creative Commons

Zeynab Taheri-Khas,

Ahmad Gharzi, Somaye Vaissi

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Март 18, 2025

Advances in cold storage and cryopreservation of amphibian sperm are critical for the genetic management conservation threatened species. This study represents first investigation into yellow spotted mountain newt (Neurergus derjugini), focusing on both short-term long-term future reproductive efforts. We examined effects seven extenders motility over time at three temperatures (4 ± 1 °C, 9 20 °C). Additionally, we assessed impact 16 cryoprotectants morphology post-thawing. Following identification most effective freezing medium, evaluated DNA fragmentation to ensure viability. Our results indicate that 10% Holtfreter's solution is optimal extender all temperatures, maintaining up 15 days 4 °C. For storage, a combination DMSO was found best preserve motility, morphology, minimize after thawing. These findings underscore importance specific temperature treatments enhancing functionality, thereby supporting successful assisted technologies (ART) endangered

Язык: Английский

Advanced sperm preservation techniques in yellow spotted mountain newts Neurergus derjugini enhance genetic management and conservation efforts DOI Creative Commons

Zeynab Taheri-Khas,

Ahmad Gharzi, Somaye Vaissi

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Март 18, 2025

Advances in cold storage and cryopreservation of amphibian sperm are critical for the genetic management conservation threatened species. This study represents first investigation into yellow spotted mountain newt (Neurergus derjugini), focusing on both short-term long-term future reproductive efforts. We examined effects seven extenders motility over time at three temperatures (4 ± 1 °C, 9 20 °C). Additionally, we assessed impact 16 cryoprotectants morphology post-thawing. Following identification most effective freezing medium, evaluated DNA fragmentation to ensure viability. Our results indicate that 10% Holtfreter's solution is optimal extender all temperatures, maintaining up 15 days 4 °C. For storage, a combination DMSO was found best preserve motility, morphology, minimize after thawing. These findings underscore importance specific temperature treatments enhancing functionality, thereby supporting successful assisted technologies (ART) endangered

Язык: Английский

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