Compounds with potentialities as novel chemotherapeutic agents in leishmaniasis at preclinical level

Experimental Parasitology, Год журнала: 2024, Номер 260, С. 108747 - 108747

Опубликована: Март 20, 2024

Язык: Английский

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Hybridization of Honeybee and Frog Peptides: Antimicrobial Peptides Composed of Apidaecins and Temporins With Enhanced Activity and Production of Their Electrospun Nanofibers

Peptide Science, Год журнала: 2024, Номер 116(5)

Опубликована: Апрель 22, 2024

ABSTRACT The increased usage and misuse of antibiotics cause antimicrobial resistance, which makes it necessary to develop new antibiotic agents. Antimicrobial peptides (AMPs) could be a significant solution addressing this issue. temporin 1Ta 1Tb peptides, derived from frog skin secretions, exhibit notable activity against Gram (+) bacteria; on the other hand, apidaecin 1b peptide, obtained honeybees, is effective (−) bacteria. In study, hybrid AMPs were synthesized by merging with produce that are active broad spectrum microorganisms. Furthermore, mixed polyvinyl alcohol (PVA) polymer bioactive nanofibers electrospinning. tested various bacteria ( E. coli ATCC 25922, P. aeruginosa 27853, S. aureus 29213, faecalis 29212). As result, found have reasonable antibacterial effect both bacterial groups. addition, peptide‐containing preserved as well. hemolytic activities also examined, was not lethal red blood cells at concentration 16 μM.

Язык: Английский

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Unraveling the role of antimicrobial peptides in plant resistance against phytopathogens

Discover Sustainability, Год журнала: 2024, Номер 5(1)

Опубликована: Сен. 5, 2024

Язык: Английский

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Design of multimodal antibiotics against intracellular infections using deep learning

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 21, 2024

Abstract The rise of antimicrobial resistance has rendered many treatments ineffective, posing serious public health challenges. Intracellular infections are particularly difficult to treat since conventional antibiotics fail neutralize pathogens hidden within human cells. However, designing molecules that penetrate cells while retaining activity historically been a major challenge. Here, we introduce APEX DUO , multimodal artificial intelligence (AI) model for generating peptides with both cell-penetrating and properties. From library 50 million AI-generated compounds, selected characterized several candidates. Our lead, Turingcin, penetrated mammalian eradicated intracellular Staphylococcus aureus . In mouse models skin abscess peritonitis, Turingcin reduced bacterial loads by up two orders magnitude. sum, generated antibiotics, opening new avenues molecular design.

Язык: Английский

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Comparative Properties of Helical and Linear Amphipathicity of Peptides Composed of Arginine, Tryptophan, and Valine

Antibiotics, Год журнала: 2024, Номер 13(10), С. 954 - 954

Опубликована: Окт. 11, 2024

The persistence of antibiotic resistance has incited a strong interest in the discovery agents with novel antimicrobial mechanisms. direct killing multidrug-resistant bacteria by cationic peptides (AMPs) underscores their importance fight against infections associated resistance. Despite vast body AMP literature demonstrating plurality structural classes, engineering been largely skewed toward idealized amphipathic helices (H-amphipathic). In contrast to helical amphipathicity, we designed series that display motifs primary structure. We previously developed rational framework for designing libraries H-amphipathic consisting Arg, Trp, and Val (H-RWV, confirmed helicity up 88% presence membrane lipids) tested most common MDR organisms.

Язык: Английский

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In Silico Hybridization and Molecular Dynamics Simulations for the Identification of Candidate Human MicroRNAs for Inhibition of Virulent Proteins' Expression in Staphylococcus aureus

Journal of Cellular Biochemistry, Год журнала: 2024, Номер unknown

Опубликована: Дек. 10, 2024

ABSTRACT Staphylococcus aureus is a major threat to human health, causing infections that range in severity from moderate fatal. The rising rates of antibiotic resistance highlight the critical need for new therapeutic techniques combat this infection. It has been recently discovered microRNAs (miRNAs) are essential cross‐kingdom communication, especially when it comes host‐pathogen interactions. demonstrated these short noncoding RNAs control gene expression gut microbiota, maintaining homeostasis; dysbiosis system linked several diseases, including cancer. Our research attempts use understanding target specific bacterial species and prevent severe diseases. In particular, we look putative miRNAs can attach virulent proteins' mRNA them being expressed. In‐silico hybridization experiments were performed between 100 miRNA sequences with varied levels gram‐positive five virulence factor genes. addition, miRNAs' binding properties investigated using molecular dynamics (MD) simulations. findings demonstrate inhibit genes, thereby opening up paths developing innovative miRNA‐based therapeutics. implementation MD simulations our study not only improves validity but also proposes method constructing therapies against life‐threatening infections.

Язык: Английский

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Classes and Applications of Cell-Penetrating Peptides

Springer eBooks, Год журнала: 2023, Номер unknown, С. 43 - 82

Опубликована: Янв. 1, 2023

Язык: Английский

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