Oxidative stress: fundamentals and advances in quantification techniques

Frontiers in Chemistry, Год журнала: 2024, Номер 12

Опубликована: Окт. 7, 2024

Oxidative species, generated endogenously via metabolism or from exogenous sources, play crucial roles in the body. At low levels, these species support immune functions by participating phagocytosis. They also aid cellular signaling and contribute to vasomodulation. However, when levels of oxidative exceed body’s antioxidant capacity neutralize them, stress occurs. This can damage macromolecules such as lipids, DNA, RNA, proteins, driving pathogenesis diseases aging through progressive deterioration physiological structures. Therefore, ability manage maintain it at optimal is essential for overall health. Understanding fundamentals stress, along with its reliable quantification, enable consistency comparability clinical practice across various diseases. While direct quantification oxidant body would be ideal assessing not feasible due their high reactivity, short half-life, challenges using conventional techniques. Alternatively, quantifying lipid peroxidation, products nucleic acids well endogenous antioxidants, serves appropriate markers indicating degree Along markers, this review discusses role novel focusing on biological samples detection Effective may enhance understanding phenomenon, aiding maintenance integrity, prevention age-associated diseases, promotion longevity.

Язык: Английский

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Kaempferol: Unveiling its anti-inflammatory properties for therapeutic innovation

Cytokine, Год журнала: 2025, Номер 186, С. 156846 - 156846

Опубликована: Янв. 5, 2025

Язык: Английский

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Impact of endoplasmic reticulum aminopeptidases 1 (ERAP1) and 2 (ERAP2) on neutrophil cellular functions

Frontiers in Cell and Developmental Biology, Год журнала: 2025, Номер 12

Опубликована: Янв. 7, 2025

Endoplasmic reticulum aminopeptidases 1 (ERAP1) and 2 (ERAP2) modulate a plethora of physiological processes for the maintenance homeostasis in different cellular subsets at both intra extracellular level. In this frame, supplementation recombinant human (rh) ERAP1 ERAP2 (300 ng/ml) was used to mimic effect stressor-induced secretion ERAPs on neutrophils isolated from 5 healthy subjects. these cells following 3 h or 24 rhERAP stimulation by Western Blot, RT-qPCR, Elisa, Confocal microscopy, transwell migration assay, Oxygraphy Flow Cytometry we assessed: i) internalization; ii) activation; iii) migration; iv) oxygen consumption rate; v) reactive species (ROS) accumulation; granule release; vi) phagocytosis; vii) autophagy. We observed that rhERAPs: were internalized neutrophils; triggered their activation as witnessed increased percentage MAC-1+CD66b+ expressing neutrophils, cytokine expression/release (IL-1β, IL-8, CCL2, TNFα, IFNγ, MIP-1β) enzyme (myeloperoxidase, Elastase); neutrophil capacity; autophagy phagocytosis activity; reduced ROS accumulation did not influence rate. Our study provides novel insights into biological role ERAPs, indicates contribute shaping promoting survival tolerance response stress-related inflammation. This information could better understanding bases governing immune responses, designing ERAP-based therapeutic protocols control neutrophil-associated diseases.

Язык: Английский

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Harnessing phytoconstituents in ethosomes: A new frontier in skin disorder management

International Journal of Pharmaceutics, Год журнала: 2025, Номер unknown, С. 125273 - 125273

Опубликована: Янв. 1, 2025

Язык: Английский

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Rainwater quality in Special Economic Zone (SEZ) in Poland

Environmental Pollution, Год журнала: 2025, Номер 370, С. 125866 - 125866

Опубликована: Фев. 19, 2025

Язык: Английский

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