Metabolites,
Год журнала:
2024,
Номер
14(6), С. 343 - 343
Опубликована: Июнь 19, 2024
The
complex
process
of
aging
leads
to
a
gradual
deterioration
in
the
function
cells,
tissues,
and
entire
organism,
thereby
increasing
risk
disease
death.
Nicotinamide
N-methyltransferase
(NNMT)
has
attracted
attention
as
potential
target
for
combating
its
related
pathologies.
Studies
have
shown
that
NNMT
activity
increases
over
time,
which
is
closely
associated
with
onset
progression
age-related
diseases.
uses
S-adenosylmethionine
(SAM)
methyl
donor
facilitate
methylation
nicotinamide
(NAM),
converting
NAM
into
S-adenosyl-L-homocysteine
(SAH)
methylnicotinamide
(MNA).
This
enzymatic
action
depletes
NAM,
precursor
adenine
dinucleotide
(NAD+),
generates
SAH,
homocysteine
(Hcy).
reduction
NAD+
levels
increase
Hcy
are
considered
important
factors
efficacy
RNA
interference
(RNAi)
therapies
small-molecule
inhibitors
targeting
demonstrates
therapeutic
target.
Despite
these
advances,
exact
mechanisms
by
influences
diseases
remain
unclear,
there
lack
clinical
trials
involving
RNAi
drugs.
Therefore,
more
in-depth
research
needed
elucidate
precise
functions
promote
development
targeted
pharmaceutical
interventions.
paper
aims
explore
specific
role
aging,
evaluate
Animals,
Год журнала:
2025,
Номер
15(3), С. 291 - 291
Опубликована: Янв. 21, 2025
Oocyte
quality
is
crucial
for
successful
fertilization
and
subsequent
embryonic
development.
Post-ovulatory
aging
leads
to
reduced
oocyte
impaired
embryogenesis,
representing
an
unavoidable
challenge
in
terms
of
certain
assisted
reproductive
techniques.
Diosmetin
(DIOS),
a
natural
flavonoid
found
lemons,
spearmint,
spider
moss,
exhibits
antioxidant,
anti-inflammatory,
anti-apoptotic
properties.
However,
its
effects
on
the
mature
porcine
oocytes
vitro
remain
unexplored.
This
study
investigated
impact
DIOS
aging.
In
IVM
medium,
fresh
were
cultured
44
h,
while
68
h.
Following
addition
varying
concentrations
(0.01,
0.1,
1
μM)
DIOS-treated
group
was
The
results
demonstrated
that
0.1
μM
significantly
improved
blastocyst
rates
cell
counts,
reactive
oxygen
species
(ROS),
elevated
glutathione
(GSH)
levels,
enhanced
mitochondrial
function,
decreased
markers
autophagy
(LC3B),
apoptosis
(annexin
V),
endoplasmic
reticulum
stress
(CHOP),
senescence
(SA-β-Gal).
Furthermore,
treatment
upregulated
expression
relevant
genes
compared
aged
group.
These
findings
suggest
effectively
delays
ACS Applied Bio Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 24, 2025
Plant
exosomes,
small
vesicles
released
by
plant
cells
that
contain
various
bioactive
molecules,
have
garnered
great
attention
for
their
potential
applications
in
medicine,
yet
delivery
skincare
remain
underexploited.
Resveratrol
(RES),
renowned
its
remarkable
antiaging
properties,
faces
challenges
transdermal
absorption
due
to
the
stratum
corneum
barrier,
hindering
efficacy.
To
address
this,
we
developed
a
system
incorporating
RES-loaded
(Leontopodium
alpinum)
exosome
(LEOEXO@RES).
Evaluation
through
both
vitro
and
vivo
experiments
demonstrated
LEOEXO@RES's
enhanced
of
compounds
multifaceted
impact.
Specifically,
this
approach
not
only
promoted
environmentally
responsible
waste
reuse
but
also
achieved
harmonious
synergy
between
LEOEXO
carriers
RES,
enhancing
overall
The
LEOEXO@RES
effectively
ameliorated
inflammation
levels
inhibited
cellular
senescence,
highlighting
promising
applications.
Journal of Cosmetic Dermatology,
Год журнала:
2025,
Номер
24(4)
Опубликована: Апрель 1, 2025
ABSTRACT
Background
Primarily
driven
by
oxidative
stress,
aging
results
from
the
attrition
of
cells,
aggravated
environmental
stressors.
Therefore,
protection
stress
is
main
target
antiaging
cosmetics.
Aims
To
evaluate
efficacy
a
unique
cosmetic
serum
combining
five
antioxidants
and
hyaluronic
acid.
Methods
The
inactivation
reactive
oxygen
species
was
evaluated
in‐tubo
.
IL‐1α
release
using
EpiDerm
TM
skin
models,
while
gene
expression
analysis
elastin
fiber
length
were
on
human
explants.
Finally,
effect
twice
daily
application
for
28
days
compared
to
those
induced
control
serum,
focusing
instrumental
assessor
evaluations.
Results
In‐tubo
,
reduces
45.2%.
A
single
topical
models
limits
UV‐induced
ROS‐mediated
release.
Compared
untreated
explants,
HB‐EGF
(heparin‐binding
epidermal
growth
factor)
homeostasis
marker
increases
22‐fold
with
treatment.
Additionally,
40.2%.
Clinically,
over
period
7
revealed
significant
improvements
in
clinical
scoring
skin's
wrinkle
(−12.8%),
smoothness
(+12.5%),
radiance
(+22.2%).
also
leads
rapid
long‐lasting
increase
hydration
(30
min:
+50.5%,
days:
+19.9%)
reduced
transepidermal
water
loss
−7.7%,
−8.7%).
highly
efficacious
well
tolerated
subjects.
Conclusion
has
antioxidant,
soothing,
photoprotective,
moisturizing
properties
that
can
be
explained
individual
its
blend
actives.
Journal of Cosmetic Dermatology,
Год журнала:
2025,
Номер
24(4)
Опубликована: Апрель 1, 2025
Bakuchiol
(BAK)
and
acetyl
hexapeptide-8
have
favorable
biological
activities
promising
applications
in
the
cosmetic
industry.
However,
evaluation
of
activity
both
alone
or
combination
has
not
been
reported.
The
was
evaluated
based
on
zebrafish
model.
Zebrafish
embryos
were
induced
to
form
models
using
different
methods.
Add
BAK
Argireline
YOUth
peptide
oil
solution
MB
(ARG,
containing
0.125%
hexapeptide-8)
treat
embryos,
they
for
their
restorative
effects
preexisting
damage
embryos.
ARG
able
reduce
water
loss
from
caudal
fin
by
0.9%
NaCl
solution.
They
alleviate
UVB-induced
decrease
expression
level
skin
tightness-related
gene
(ELN/COL1a1b)
effectively
reversed
increase
β-galactosidase
exposure
H2O2
restored
telomerase
In
addition,
counteract
oxidative
stress
mitochondrial
as
a
result
LPS
treatment.
Finally,
also
effective
suppressing
neutrophil
counts
inflammatory
cytokine
levels
due
exposure.
Notably,
more
when
used
combination.
Acetyl
promotes
bioactivity
(Danio
rerio).
moisturizing,
anti-inflammatory,
antioxidant,
antioxidant
bioactivities
OBM Geriatrics,
Год журнала:
2025,
Номер
09(02), С. 1 - 32
Опубликована: Апрель 14, 2025
Cellular
senescence
(aging)
is
a
physiological
process
that
plays
role
in
tissue
remodeling,
wound
healing,
and
embryogenesis.
Prolonged
of
cells
can
have
detrimental
effects
trigger
secretory
phenotype
(SASP,
senescence-associated
phenotype),
degenerative
disorders,
cancer,
age-related
diseases.
Suitable
biomarkers
range
different
laboratory
methods
are
used
to
investigate
these
complex
relationships
vitro
vivo.
Since
universal
biomarker
for
cell
has
not
yet
been
identified,
numerous
identify
senescent
cell.
The
detection
quantification
their
SASP
provide
the
basis
targeted
treatment
patient.
In
parallel,
single-cell
analysis
also
required
quantitative
assessment
therapy
result.
Depending
on
facilities
performing
analysis,
wide
available.
this
review,
we
general
overview
accessible
techniques
such
as
immunohistochemistry
using
microscopy
automated
flow
cytometry
introduce
new
possibilities
by
modern
like
mass
spectrometry
or
genetic
method
single
cells.
focus
here
use
routine
laboratories.
classical
with
enzyme
immunoassays,
measurement
products
(IL-6,
IL-8),
part
work.
This
review
discusses
ideas
visualisation
clinical
patient
data
gerontology.
An
outlook
potential
future
optimization
improve
rejuvenate
status
patients
cellular
organ-specific
level
discussed.
Biology,
Год журнала:
2024,
Номер
13(9), С. 647 - 647
Опубликована: Авг. 23, 2024
The
skin
is
the
layer
of
tissue
that
covers
largest
part
body
in
vertebrates,
and
its
main
function
to
act
as
a
protective
barrier
against
external
environmental
factors,
such
microorganisms,
ultraviolet
light
mechanical
damage.
Due
important
function,
investigating
factors
lead
aging
age-related
diseases,
well
understanding
biology
this
process,
high
importance.
Indeed,
it
has
been
reported
several
internal
stressors
contribute
aging,
similar
other
tissues.
Moreover,
during
senescent
cells
accumulate
express
senescence-associated
which
paracrine
manner
on
neighboring
healthy
In
review,
we
will
present
cellular
senescence,
ways
study
senescence
vitro
vivo.
We
further
discuss
adverse
effects
accumulation
chronic
therapeutic
agents
tools
selectively
target
eliminate
them.
Diagnostics,
Год журнала:
2024,
Номер
14(11), С. 1126 - 1126
Опубликована: Май 29, 2024
The
soft
superficial
tissues
of
the
face
are
against
gravity
through
an
intricate
network
ligaments
and
ligamentous
attachments.
aim
this
investigation
is
to
delineate
relationship
between
muscular,
fibrous,
vascular
components
musculoaponeurotic
system
(SMAS)
at
level
its
periosteal
fixation
areas
from
advanced
radiological
novel
biomarkers’
perspectives.
These
represent
key
points
underlying
skin
aging
longevity
restorative
surgery
results.
Methods:
This
study
was
carried
out
on
37
surgical
specimens,
excised
patients
admitted
for
surgery.
On
we
used
special
immunohistochemical
techniques,
such
as
markers
collagen
type
III,
angiogenesis,
endothelium
(I-CAM2)
muscle
fibers
(MYH2).
We
performed
a
micro-CT
evaluation
these
specimens.
Results:
results
showed
different
radiologic
IHC
characteristics
means
SMAS.
Evidence
morphohistological
peculiarities
retaining
highlights
new
data
future
functional
studies
structures.
Our
research
must
be
continued
with
larger
groups
subjects
detailed
methodological
microperfusion
could
important
step
in
biotissue
engineering
customization
techniques
involving
sub-SMAS
layers.
