The
developed
multifunctional
fluorescent
probe
enables
the
simultaneous
detection
of
chymotrypsin
as
a
model
protease
and
hydrogen
peroxide
representative
reactive
oxygen
species
(ROS)
in
biologically
relevant
concentration
ranges.
sensing
is
based
on
cleavage
its
selectively
recognizable
peptide
sequence
consequent
disruption
FRET
between
coumarin
(DEAC)
fluorescein
(FL).
Analogously,
presence
causes
gradual
degradation
H2O2-labile
benzopyrylium-coumarin
(BC)
dye.
Considering
fluorescence
emission
responses
individual
chymotrypsin-peroxide
probe-attached
fluorophores
after
their
excitation
at
425
nm,
sole
either
(50–1000
ng/mL)
or
(10–200
μM)
sample
could
be
unambiguously
confirmed
refuted.
In
addition,
reliable
approximate
quantification
both
studied
ranges
100–1000
ng/mL
20–200
μM
for
H2O2,
respectively,
performed
well.
obtained
results
are
summarized
visualized
graphical
models.
Cell Communication and Signaling,
Год журнала:
2024,
Номер
22(1)
Опубликована: Янв. 2, 2024
Abstract
Cancer
remains
a
significant
global
public
health
concern,
with
increasing
incidence
and
mortality
rates
worldwide.
Oxidative
stress,
characterized
by
the
production
of
reactive
oxygen
species
(ROS)
within
cells,
plays
critical
role
in
development
cancer
affecting
genomic
stability
signaling
pathways
cellular
microenvironment.
Elevated
levels
ROS
disrupt
homeostasis
contribute
to
loss
normal
functions,
which
are
associated
initiation
progression
various
types
cancer.
In
this
review,
we
have
focused
on
elucidating
downstream
that
influenced
oxidative
stress
carcinogenesis.
These
include
p53,
Keap1-NRF2,
RB1,
p21,
APC,
tumor
suppressor
genes,
cell
type
transitions.
Dysregulation
these
can
lead
uncontrolled
growth,
impaired
DNA
repair
mechanisms,
evasion
death,
all
hallmark
features
development.
Therapeutic
strategies
aimed
at
targeting
emerged
as
area
investigation
for
molecular
biologists.
The
objective
is
limit
response
time
cancer,
including
liver,
breast,
prostate,
ovarian,
lung
cancers.
By
modulating
redox
balance
restoring
homeostasis,
it
may
be
possible
mitigate
damaging
effects
enhance
efficacy
treatments.
targeted
therapies
interventions
specifically
address
impact
holds
great
promise
improving
patient
outcomes.
approaches
antioxidant-based
treatments,
redox-modulating
agents,
restore
function
affected
stress.
summary,
understanding
carcinogenesis
process
through
therapeutic
utmost
importance
combating
Further
research
needed
unravel
complex
mechanisms
underlying
stress-related
develop
effective
translated
into
clinical
applications
management
treatment
Antioxidants,
Год журнала:
2025,
Номер
14(1), С. 94 - 94
Опубликована: Янв. 15, 2025
Normal
tissues
typically
maintain
partial
oxygen
pressure
within
a
range
of
3–10%
oxygen,
ensuring
homeostasis
through
well-regulated
supply
and
responsive
vascular
network.
However,
in
solid
tumors,
rapid
growth
often
outpaces
angiogenesis,
creating
hypoxic
microenvironment
that
fosters
tumor
progression,
altered
metabolism
resistance
to
therapy.
Hypoxic
regions
experience
uneven
distribution
with
severe
hypoxia
the
core
due
poor
vascularization
high
metabolic
consumption.
Cancer
cells
adapt
these
conditions
shifts,
predominantly
relying
on
glycolysis,
by
upregulating
antioxidant
defenses
mitigate
reactive
species
(ROS)-induced
oxidative
damage.
Hypoxia-induced
ROS,
resulting
from
mitochondrial
dysfunction
enzyme
activation,
exacerbates
genomic
instability,
aggressiveness,
therapy
resistance.
Overcoming
hypoxia-induced
ROS
cancer
requires
multifaceted
approach
targets
various
aspects
biology.
Emerging
therapeutic
strategies
target
resistance,
focusing
hypoxia-inducible
factors,
levels,
subpopulations.
Combining
innovative
therapies
existing
treatments
holds
promise
for
improving
outcomes
overcoming
mechanisms.
Nutrients,
Год журнала:
2023,
Номер
15(20), С. 4486 - 4486
Опубликована: Окт. 23, 2023
Resveratrol
is
a
stilbenoid
from
red
grapes
that
possesses
strong
antioxidant
activity.
has
been
shown
to
have
anticancer
activity,
making
it
promising
drug
for
the
treatment
and
prevention
of
numerous
cancers.
Several
in
vitro
vivo
investigations
validated
resveratrol’s
capabilities,
demonstrating
its
ability
block
all
steps
carcinogenesis
(such
as
initiation,
promotion,
progression).
Additionally,
resveratrol
found
auxiliary
pharmacological
effects
such
anti-inflammatory,
cardioprotective,
neuroprotective
Despite
properties,
several
obstacles,
poor
solubility
bioavailability,
well
adverse
effects,
continue
be
key
obstacles
development.
This
review
critically
evaluates
clinical
trials
date
aims
develop
framework
into
clinically
viable
drug.
The
intricate
relationship
between
cancer,
circadian
rhythms,
and
aging
is
increasingly
recognized
as
a
critical
factor
in
understanding
the
mechanisms
underlying
tumorigenesis
cancer
progression.
Aging
well-established
primary
risk
for
while
disruptions
rhythms
are
intricately
associated
with
progression
of
various
tumors.
Moreover,
itself
disrupts
leading
to
physiological
changes
that
may
accelerate
development.
Despite
these
connections,
specific
interplay
processes
their
collective
impact
on
remains
inadequately
explored
literature.
In
this
review,
we
systematically
explore
influence
We
discuss
how
core
genes
tumor
prognosis,
highlighting
shared
hallmarks
such
genomic
instability,
cellular
senescence,
chronic
inflammation.
Furthermore,
examine
aging,
focusing
crosstalk
contributes
tumorigenesis,
proliferation,
apoptosis,
well
metabolism
stability.
By
elucidating
common
pathways
linking
review
provides
new
insights
into
pathophysiology
identifies
potential
therapeutic
strategies.
propose
targeting
regulation
could
pave
way
novel
treatments,
including
chronotherapy
antiaging
interventions,
which
offer
important
benefits
clinical
management
cancer.
Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics,
Год журнала:
2024,
Номер
32(5), С. 831 - 847
Опубликована: Янв. 1, 2024
Ovarian
cancer
is
among
the
most
lethal
gynecological
cancers,
primarily
due
to
lack
of
specific
symptoms
leading
an
advanced-stage
diagnosis
and
resistance
chemotherapy.Drug
(DR)
poses
significant
challenge
in
treating
patients
with
existing
drugs.The
Food
Drug
Administration
(FDA)
has
recently
approved
three
new
therapeutic
drugs,
including
two
poly
(ADP-ribose)
polymerase
(PARP)
inhibitors
(olaparib
niraparib)
one
vascular
endothelial
growth
factor
(VEGF)
inhibitor
(bevacizumab)
for
maintenance
therapy.However,
these
drugs
emerged.Therefore,
understanding
mechanisms
DR
exploring
approaches
overcome
them
crucial
effective
management.In
this
review,
we
summarize
major
molecular
discuss
novel
strategies
combat
DR.
Frontiers in Pharmacology,
Год журнала:
2025,
Номер
16
Опубликована: Март 19, 2025
Introduction
Colorectal
cancer
is
a
leading
cause
of
related-death
worldwide,
and
resistance
to
5-fluorouracil
(5FU,
key
component
chemotherapy
regimens,
major
clinical
concern.
We
have
previously
elucidated
the
effects
Rhus
coriaria
ethanolic
extract
(RCE)
in
triple-negative
breast
cancer,
CRC,
pancreatic
cells.
Here,
we
explored
anticancer
RCE
parental
(HCT-116-WT)
5FU-resistant
HCT-116
(HCT-116-5FU-R)
CRC
Methods
MTT
assay
was
used
assess
cell
viability.
Muse
analyzer
viability,
cycle
distribution,
apoptosis.
Additionally,
colony
formation
growth
assays
western
blots
were
performed.
In
vivo
assessed
by
an
ovo
chick
embryo
tumor
assay.
Results
found
that
inhibited
viability
capacities
HCT-116-WT
HCT-116-5FU-R
The
antiproliferative
attributed
DNA
damage-mediated
impairment
at
S
phase,
induction
Beclin-1-independent
autophagy
both
lines.
Mechanistically,
inhibition
mTOR,
STAT3
p38
MAPK
pathways
implicated
latter.
induced
caspase-7-independent
apoptosis
However,
cells
resistant
through
upregulation
survivin,
downregulation
Bax.
Using
proteasome
inhibitors,
clarified
pathway
contributed
RCE-mediated
death
Lastly,
confirmed
xenografts
model.
Discussion
Collectively,
our
findings
highlight
source
phytochemicals
can
be
as
agents
for
CRC.
Antioxidants,
Год журнала:
2025,
Номер
14(1), С. 115 - 115
Опубликована: Янв. 20, 2025
The
role
of
mitochondria
as
the
electric
engine
cells
is
well
established.
Over
past
two
decades,
accumulating
evidence
has
pointed
out
that,
despite
presence
a
highly
active
glycolytic
pathway
(Warburg
effect),
functional
and
even
upregulated
mitochondrial
respiration
occurs
in
cancer
to
meet
need
high
energy
biosynthetic
demand
sustain
their
anabolic
growth.
Mitochondria
are
also
primary
source
intracellular
ROS.
Cancer
maintain
moderate
levels
ROS
promote
tumorigenesis,
metastasis,
drug
resistance;
indeed,
once
cytotoxicity
threshold
exceeded,
trigger
oxidative
damage,
ultimately
leading
cell
death.
Based
on
this,
metabolic
functions
generation
considered
attractive
targets
synthetic
natural
anticancer
compounds.
Tocotrienols
(TTs),
specifically
δ-
γ-TT
isoforms,
vitamin
E-derived
biomolecules
widely
shown
possess
striking
properties
since
they
regulate
several
molecular
pathways.
Herein,
we
provide
for
first
time
an
overview
reprogramming
redox
homeostasis
perturbation
occurring
cells,
highlighting
involvement
TTs.
This
sheds
light
use
these
compounds
promising
preventive
or
therapeutic
approach
novel
strategies.
