17β-Estradiol Stimulates Oxidative Stress Components and Thyroid Specific Genes in Porcine Thyroid Follicular Cells: Potential Differences Between Sexes DOI Creative Commons
Jan Stępniak, Małgorzata Karbownik‐Lewińska

Cells, Год журнала: 2024, Номер 13(21), С. 1769 - 1769

Опубликована: Окт. 25, 2024

17β-estradiol plays a crucial role in regulating cellular processes both reproductive and non-reproductive tissues, including the thyroid gland. It modulates oxidative stress contributes to sexual dimorphism diseases, with ROS production, particularly H2O2, generated by NOX/DUOX enzymes. This study aimed investigate effects of (10 nM or 100 nM) on expression NOX/DUOX, thyroid-specific genes, endoplasmic reticulum (ER) stress-related genes male female porcine follicular cells. Expression studied was evaluated RT-PCR before after treatment alone addition NOX4 inhibitor (GKT-136901). Additionally, level measured flow cytometry analysis. Our results show that significantly upregulates TPO, stimulates expression, affecting redox state also ER such as CHOP. In conclusion, estrogen excess may contribute disease development via possible mechanisms upregulation key involved response stress, especially CHOP, well stimulation system consequent overproduction. These play certain higher prevalence diseases women.

Язык: Английский

Corneal endothelial dysfunction treatments: Recent advances in non-invasive treatment strategies DOI Creative Commons
Nataliia Gnyliukh, Rabah Boukherroub, Sabine Szunerits

и другие.

Nano Today, Год журнала: 2025, Номер 63, С. 102740 - 102740

Опубликована: Апрель 4, 2025

Язык: Английский

Процитировано

0
Emerging alternatives to keratoplasty for corneal endothelial cell dysfunction DOI
Ron Kaufman, Albert S. Jun

Current Opinion in Ophthalmology, Год журнала: 2024, Номер 35(5), С. 415 - 422

Опубликована: Июнь 26, 2024

Purpose of review While effective for treating endothelial dysfunction, keratoplasty has shortcomings including limited access to donor tissue much the world. Thus, alternative strategies are under development. This explores main advancements achieved in this field during 2022–2023. Recent findings publications further support validity intracameral cultivated allogeneic cell injection and Descemet stripping only, while emphasizing benefits adjunctive Rho-associated kinase inhibitor (ROCKi) therapy. New donor-independent artificial implants, such as EndoArt, show favorable results. Multiple pharmacologic agents, especially ROCKi, promise monotherapies, yet none currently approved human treatment. regenerative genetic therapies being investigated but all still preclinical stages. Summary A plethora innovative alternatives disease is Among these, surgical methods mainstay treatment closest clinical application, though studies establish their over needed. Albeit promising, pharmacologic, regenerative, approaches require validation farther from application.

Язык: Английский

Процитировано

3
17β-Estradiol Stimulates Oxidative Stress Components and Thyroid Specific Genes in Porcine Thyroid Follicular Cells: Potential Differences Between Sexes DOI Creative Commons
Jan Stępniak, Małgorzata Karbownik‐Lewińska

Cells, Год журнала: 2024, Номер 13(21), С. 1769 - 1769

Опубликована: Окт. 25, 2024

17β-estradiol plays a crucial role in regulating cellular processes both reproductive and non-reproductive tissues, including the thyroid gland. It modulates oxidative stress contributes to sexual dimorphism diseases, with ROS production, particularly H2O2, generated by NOX/DUOX enzymes. This study aimed investigate effects of (10 nM or 100 nM) on expression NOX/DUOX, thyroid-specific genes, endoplasmic reticulum (ER) stress-related genes male female porcine follicular cells. Expression studied was evaluated RT-PCR before after treatment alone addition NOX4 inhibitor (GKT-136901). Additionally, level measured flow cytometry analysis. Our results show that significantly upregulates TPO, stimulates expression, affecting redox state also ER such as CHOP. In conclusion, estrogen excess may contribute disease development via possible mechanisms upregulation key involved response stress, especially CHOP, well stimulation system consequent overproduction. These play certain higher prevalence diseases women.

Язык: Английский

Процитировано

1