Marine and Freshwater Behaviour and Physiology, Год журнала: 2024, Номер unknown, С. 1 - 20
Опубликована: Окт. 3, 2024
Язык: Английский
Animals, Год журнала: 2024, Номер 14(3), С. 411 - 411
Опубликована: Янв. 26, 2024
Through a 30-day experiment, this study investigated the effects of five photoperiods (0L:24D, 6L:18D, 12L:12D, 18L:6D, and 24L:0D) on survival, enzyme activity, body color, growth-related gene expression redclaw crayfish (Cherax quadricarinatus) juveniles. The results showed that C. quadricarinatus juveniles under 18L:6D 24L:0D exhibited highest survival rate, which was significantly higher than rates other three (p < 0.05). However, 0L:24D group had final weight gain surpassing those groups Regarding activity hormone levels, photoperiod relatively superoxide dismutase (SOD), acid phosphatase (ACP), lysozyme (LZM) enzymes photoperiods, but their levels melatonin cortisol were low. In addition, malondialdehyde (MDA) content. Analysis revealed retinoid X receptor (RXR) α-amylase (α-AMY) genes in four With increasing daylight exposure, color changed from pale blue to yellow–brown. summary, achieved high rates, good growth performance, strong antioxidant stress response, immune defense capabilities an 18 h photoperiod. Therefore, industrial seedling cultivation crayfish, it is recommended provide daily light. Further, demonstrated ability manipulate through controlled artificial photoperiods. These findings essential technical parameters needed for
Язык: Английский
Процитировано
5
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(12), С. 6809 - 6809
Опубликована: Июнь 20, 2024
Macrobrachium rosenbergii is an essential species for freshwater economic aquaculture in China, but the larval process, their salinity requirement high, which leads to stress water. In order elucidate mechanisms regulating response of M. acute low-salinity exposure, we conducted a comprehensive study exposed different salinities’ (0‰, 6‰, and 12‰) data 120 h. The activities catalase, superoxide dismutase, glutathione peroxidase were found be significantly inhibited hepatopancreas muscle following resulting oxidative damage immune deficits rosenbergii. Differential gene enrichment transcriptomics indicated that induced metabolic differences inflammatory dysfunction differential expressions MIH, JHEH, EcR genes inhibition growth, development, molting ability At proteomic level, low affected biological cellular regulation, as well response. Tyramine, trans-1,2-Cyclohexanediol, sorbitol, acetylcholine chloride, chloroquine screened by metabolomics markers. addition, combined multi-omics analysis revealed metabolite was highly correlated with low-salt stress.
Язык: Английский
Процитировано
3
Aquaculture Reports, Год журнала: 2025, Номер 40, С. 102608 - 102608
Опубликована: Янв. 5, 2025
Язык: Английский
Процитировано
0
Aquaculture Reports, Год журнала: 2025, Номер 42, С. 102788 - 102788
Опубликована: Апрель 5, 2025
Язык: Английский
Процитировано
0
Frontiers in Marine Science, Год журнала: 2025, Номер 12
Опубликована: Май 20, 2025
Myostatin ( Mstn ), a negative regulatory factor in myocytes, has garnered significant attention. This study focused on the gene and its 24-base-pair mutant promoter region of giant freshwater prawn Macrobrachium rosenbergii aiming to investigate roles M. . The research provides theoretical insights into functional mechanisms crustaceans. Spatiotemporal expression patterns revealed presence throughout embryonic development various body tissues, with peak during gastrula stage hepatopancreas. During molting cycle, levels decreased order post-molt (A phase), pre-molt (D3 (E phase). Knockdown experiments targeting two genotypes within significantly reduced growth rates extended cycles compared control group. Further trials F2 generation confirmed these findings, highlighting that knockdown influenced three molting-related genes slowed rate clarifies role crustacean molting, providing foundation for understanding offering potential applications aquaculture.
Язык: Английский
Процитировано
0
Journal of Marine Science and Engineering, Год журнала: 2024, Номер 12(8), С. 1387 - 1387
Опубликована: Авг. 13, 2024
Salinity is a crucial environmental factor influencing the survival, growth, development, and reproduction of aquatic animals. However, underlying molecular mechanisms shrimp’s response to salinity stress are not yet fully understood. Therefore, we used Illumina NovaSeq 6000 platform perform transcriptome sequencing hepatopancreas Litopenaeus vannamei under high-salinity (30 PSU), medium-salinity (10 low-salinity (0.5 PSU) conditions. We obtained 63.23 Gb high-quality data identified 3589 differentially expressed genes (DEGs), including 1638 upregulated 1951 downregulated genes. Notably, comparison between control group revealed that BBOX1 CHE1 were significantly upregulated, while ACOX1, MPV, CYP2L1, GCH, MVK, TREt1, XDH downregulated. These primarily involved in key metabolic pathways, such as fatty acid oxidation, cholesterol metabolism, hormone synthesis metabolism. The β-oxidation, ACAD, HADH, HSD17B4, PECR, CROT, PIPOX, CG5009, all showed downward trend, suggesting L. may respond salt by regulating oxidative optimizing energy utilization, maintaining cell homeostasis Functional annotation gene ontology (GO) KEGG pathway enrichment analysis highlighted roles these significant DEGs adaptation environments varying salinity, underscoring importance pathways their adaptive physiological responses. This study provides biological basis for understanding protection strategies stress.
Язык: Английский
Процитировано
1
Journal of Experimental Zoology Part A Ecological and Integrative Physiology, Год журнала: 2024, Номер unknown
Опубликована: Ноя. 6, 2024
Bivalve mollusks frequently experience salinity fluctuations that may drive oxidative stress (OS) in the organism. Here we investigated OS markers and histopathological changes gills hemolymph of Mediterranean mussels Mytilus galloprovincialis Lamarck, 1819 exposed to a wide range salinities (6, 10, 14, 24, 30 ppt). Mussels were captured at shellfish farm with 18 ppt then hypo- hypersaline conditions laboratory. Indicators redox balance hemocytes (intracellular reactive oxygen species (ROS) levels, DNA damage) (thiobarbituric acid substances (TBARS), protein carbonyls (PC), activity catalase (CAT), superoxide dismutase (SOD) glutathione peroxidase (GPx) measured. The effect on microstructure has been evaluated as well. results revealed induction tissues cells for both experimental increase decrease modelings. Hemocytes showed higher sensitivity osmotic compared gills. In TBARS stable all groups PC increased only 6 ppt. SOD, CAT GPx decreased acclimated 24 further salinisation up was associated recovery enzymes. Major upon included inflammatory reactions, circulatory alterations, regressive progressive changes. Our findings clearly indicate promote cellular tissue level also affect mussels. provide new insights into mechanisms bivalves.
Язык: Английский
Процитировано
1
Animals, Год журнала: 2024, Номер 14(13), С. 1880 - 1880
Опубликована: Июнь 26, 2024
Metabolomics has been used extensively to identify crucial molecules and biochemical effects induced by environmental factors. To understand the of acute low-salinity stress on
Язык: Английский
Процитировано
0
Marine and Freshwater Behaviour and Physiology, Год журнала: 2024, Номер unknown, С. 1 - 20
Опубликована: Окт. 3, 2024
Язык: Английский
Процитировано
0