Advances in Blood Biomarkers for Alzheimer’s Disease: Ultra-Sensitive Detection Technologies and Impact on Clinical Diagnosis
Degenerative Neurological and Neuromuscular Disease,
Год журнала:
2024,
Номер
Volume 14, С. 85 - 102
Опубликована: Июль 1, 2024
Abstract:
Alzheimer's
disease
has
escalated
into
a
critical
public
health
concern,
marked
by
its
neurodegenerative
nature
that
progressively
diminishes
cognitive
abilities.
Recognized
as
continuously
advancing
and
presently
incurable
condition,
AD
underscores
the
necessity
for
early-stage
diagnosis
interventions
aimed
at
delaying
decline
in
mental
function.
Despite
proven
efficacy
of
cerebrospinal
fluid
positron
emission
tomography
diagnosing
AD,
their
broader
utility
is
constrained
significant
costs
invasive
these
procedures.
Consequently,
innovation
blood
biomarkers
such
Amyloid-beta,
phosphorylated-tau,
total-tau
et
al,
distinguished
high
sensitivity,
minimal
invasiveness,
accessibility,
cost-efficiency,
emerges
promising
avenue
diagnosis.
The
advent
ultra-sensitive
detection
methodologies,
including
single-molecule
enzyme-linked
immunosorbent
assay
immunoprecipitation-mass
spectrometry,
revolutionized
plasma
biomarkers,
supplanting
previous
low-sensitivity
techniques.
This
rapid
advancement
technology
facilitates
more
accurate
quantification
pathological
brain
proteins
AD-associated
bloodstream.
manuscript
meticulously
reviews
landscape
current
research
on
immunological
markers
anchored
National
Institute
Aging—Alzheimer's
Association
AT(N)
framework.
It
highlights
selection
forefront
technologies
now
integral
to
assessing
markers.
Additionally,
this
review
examines
crucial
pre-analytical
processing
steps
samples
significantly
impact
outcomes
addresses
practical
challenges
faced
during
clinical
testing.
These
discussions
are
enhancing
our
comprehension
refining
diagnostic
precision
using
blood-based
biomarkers.
aims
shed
light
potential
avenues
improvement
techniques
employed
detecting
investigating
thereby
contributing
field
research.
Keywords:
disease,
blood,
technologies,
Язык: Английский
Electrochemical Biosensors for the Detection of Exosomal microRNA Biomarkers for Early Diagnosis of Neurodegenerative Diseases
Jiacheng Yu,
Runzhi Zhou,
Shan Liu
и другие.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 9, 2025
Early
and
precise
diagnosis
of
neurodegenerative
disorders
like
Alzheimer's
(AD)
Parkinson's
(PD)
is
crucial
for
slowing
their
progression
enhancing
patient
outcomes.
Exosomal
microRNAs
(miRNAs)
are
emerging
as
promising
biomarkers
due
to
ability
reflect
the
diseases'
pathology,
yet
low
abundance
poses
significant
detection
hurdles.
This
review
article
delves
into
burgeoning
field
electrochemical
biosensors,
designed
exosomal
miRNA
biomarkers.
Electrochemical
biosensors
offer
a
compelling
solution,
combining
sensitivity
required
detect
low-abundance
with
specificity
needed
discern
profiles
distinctive
neural
pathological
states.
We
explore
operational
principles
these
including
transduction
mechanisms
that
facilitate
detection.
The
also
summarizes
advancements
in
nanotechnology,
signal
enhancement,
bioreceptor
anchoring,
microfluidic
integration
improve
sensor
accuracy.
evidence
use
disease
analyzed,
focusing
on
clinical
impact,
diagnostic
precision,
obstacles
faced
practical
applications.
Their
potential
point-of-care
testing
regulatory
considerations
market
entry
discussed.
Looking
toward
future,
highlights
forthcoming
innovations
might
revolutionize
early
processes.
impressive
sensitivity,
specificity,
compatibility,
track
become
instrumental
diseases,
possibly
transforming
care
prognosis.
Язык: Английский
Advancing Alzheimer’s disease therapy through engineered exosomal Macromolecules
Brain Research,
Год журнала:
2025,
Номер
unknown, С. 149590 - 149590
Опубликована: Март 1, 2025
Язык: Английский
A Quantum Chemical Study on the Antioxidant Properties of Myricetin, Quercetin, and Kaempferol Using Density Functional Theory (DFT) and Molecular Docking
Cumhuriyet Science Journal,
Год журнала:
2024,
Номер
45(4), С. 740 - 749
Опубликована: Дек. 30, 2024
This
study
explores
the
antioxidant
properties
of
myricetin,
quercetin,
and
kaempferol
using
density
functional
theory
(DFT)
molecular
docking
analyses.
The
findings
this
not
only
highlight
significant
potential
flavonoids
provide
a
foundation
for
clinical
applications
future
research
aimed
at
optimizing
their
pharmacokinetic
therapeutic
use.
optimized
geometries
these
were
determined
through
DFT
calculations
B3LYP/6-31G(d,p)
level.
Key
electronic
properties,
such
as
total
energy
(Eₜ),
zero-point
(ZPE),
enthalpy
(H),
entropy
(S),
dipole
moment
(μ),
HOMO-LUMO
gaps
(ΔE_H-L),
computed
to
evaluate
stability
reactivity.
Molecular
with
proteins
1HD2,
1RAU,
3FZS,
3GRS
revealed
strong
binding
affinities,
particularly
which
achieved
scores
-5.330
1HD2
-8.652
3GRS,
indicating
high
activity.
ADME
analyses
demonstrated
that
exhibited
most
favorable
profile,
human
oral
absorption
rate
63.637%
compliance
Lipinski’s
Rule
Five.
Conversely,
myricetin
showed
reduced
bioavailability
despite
its
affinity.
work
emphasizes
flavonoid
pharmacokinetics
maximize
while
reinforcing
relevance
in
therapy.
Язык: Английский