Proteomic analysis reveals the sperm protein difference among fresh and frozen semen of Niangya yak in Xizang DOI Creative Commons

Kui-yuan Su,

Hanyu Fu,

Yefen Xu

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Дек. 3, 2024

Abstract Background To enhance Niangya yak's reproductive efficiency and breeding process, this study employed Label-free quantitative proteomics technology to analyze fresh (F) chilled (C) semen samples.Result The analysis revealed an extensive repertoire of 18,338 peptide segments 2,579 proteins within yak semen. Among them, 2,300 were successfully annotated in the database. Notably, a total 300 DEPs identified between C F groups; comprising 129 up-regulated 171 down-regulated DEPs. GO enrichment highlighted that these may be involved lipid metabolism as well protein binding with proteases phosphorylation processes associated sperm cryodamage. KEGG showed 35 mapped pathways related motility, plasma membrane integrity apoptosis. Specifically, ribosome pathway, glycerophospholipid ferroptosis phospholipase D signaling pathway might closely linked decreased vitality, damaged integrity, apoptosis induced by freezing Based on PPI groups F, has preliminarily potential HSPBP1, HSP90AB1, CCDC83, PMSD4, ISYNA1, KLHL10, UAB1 are vitality cell caused cryodamage.Conclusion This represents first their involvement biological frozen using technology. It provides theoretical foundation for comprehending mechanism cryodamage enhancing yaks.

Язык: Английский

Nicotinamide Mononucleotide Enhances Porcine Sperm Quality by Activating the SIRT3-SOD2/ROS Pathway and Promoting Oxidative Phosphorylation DOI

H. Zhang,

Xue Qin,

Nataraj Bojan

и другие.

Animal Reproduction Science, Год журнала: 2025, Номер 275, С. 107797 - 107797

Опубликована: Фев. 15, 2025

Язык: Английский

Процитировано

0
5-Aminolevulinate acid improves boar semen quality by enhancing the sperm mitochondrial function DOI
Shanpeng Wang, Qi Wang, Xuejun Zeng

и другие.

Theriogenology, Год журнала: 2025, Номер 239, С. 117389 - 117389

Опубликована: Март 12, 2025

Язык: Английский

Процитировано

0
Crocin-I mitigates diquat-induced pulmonary fibrosis via activation of the SIRT3/FOXO3a pathway DOI

Shuangyun Xi,

Xiuli Li, Weijun Chen

и другие.

Biomedicine & Pharmacotherapy, Год журнала: 2025, Номер 186, С. 118043 - 118043

Опубликована: Апрель 8, 2025

Язык: Английский

Процитировано

0
Nicotinamide mononucleotide promotes female germline stem cell proliferation by activating the H4K16ac-Hmgb1-Fyn-PLD signaling pathway through epigenetic remodeling DOI Creative Commons
Hong Zhou, Yujie Liu,

Geng G. Tian

и другие.

Cell & Bioscience, Год журнала: 2025, Номер 15(1)

Опубликована: Апрель 17, 2025

Abstract Background Nicotinamide mononucleotide (NMN), an endogenous nucleotide essential for various physiological processes, has unclear role and regulatory mechanisms in female germline stem cell (FGSC) development. Results We demonstrate that NMN significantly enhances FGSC viability proliferation. Quantitative acetylation proteomics revealed markedly increases the of histone H4 at lysine 16 (H4K16ac). Subsequent chromatin immunoprecipitation sequencing (ChIP-seq) RNA (RNA-seq) identified high mobility group box 1 ( Hmgb1 ) as a downstream target H4K16ac, finding further validated by ChIP-qPCR. Knockdown reduced proliferation disrupting cycle progression, inducing apoptosis, decreasing accessibility. High-throughput chromosome conformation capture (Hi-C) analysis showed knockdown induced A/B compartment switching, increased number topologically associating domains (TADs), decreased loop formation FGSCs. Notably, promoter region Fyn proto-oncogene disappeared following knockdown. ChIP-qPCR dual-luciferase reporter assays confirmed interaction between promoter. Importantly, overexpression reversed inhibitory effects on Proteomic suggested this rescue was mediated through phospholipase D (PLD) signaling pathway, selectively enhanced phosphorylation PLD1 threonine 147 without affecting serine 561. Furthermore, treatment with 5-fluoro-2-indolyldechlorohaloamide, PLD inhibitor, nullified pro-proliferative overexpression. Conclusions Our findings reveal promotes activating H4K16ac- - -PLD pathway epigenetic remodeling. These results deepen our understanding highlight potential therapeutic avenues advancing applications reproductive medicine.

Язык: Английский

Процитировано

0
Proteomic analysis reveals the sperm protein difference among low, high quality and frozen semen of Sewa sheep in Tibet DOI

Kui-yuan Su,

Han-yu Fu,

Yefen Xu

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Сен. 6, 2024

Abstract The Tibetan Sewa sheep is a unique breed that inhabits the plateau area at an altitude of approximately 4,800 m. In this study, we conducted DIA quantitative proteomic analysis on high-quality semen (F), frozen (C), and low-quality (L) from sheep. A total 33,073 peptide segments 3,175 proteins were detected in three groups semen. Among them, 3,700 functionally annotated database. Comparison between C F revealed 163 differentially expressed (DEPs) with 134 upregulated 29 downregulated proteins. These DEPs found to be associated biological regulation metabolic processes Gene Ontology (GO) enrichment analysis; they also mapped 97 pathways Kyoto Encyclopedia Genes Genomes (KEGG) analysis. L identified 459 319 140 cellular component biogenesis GO 107 KEGG Protein-protein interaction (PPI) based comparisons vs group potential may affect sperm cryotolerance vitality; however, their specific functional mechanisms require further investigation. We have elucidated for first time various types as well involvement pathways, providing theoretical basis understanding quality freezing damage

Язык: Английский

Процитировано

0
β-Nicotinamide Mononucleotide Alleviates Sepsis-associated Acute Kidney Injury by Activating NAD+/SIRT3 Signaling DOI

Chengcheng Sun,

Hui Xiong, Guoqin Chen

и другие.

Cell Biochemistry and Biophysics, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 23, 2024

Язык: Английский

Процитировано

0
Proteomic analysis reveals the sperm protein difference among fresh and frozen semen of Niangya yak in Xizang DOI Creative Commons

Kui-yuan Su,

Hanyu Fu,

Yefen Xu

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Дек. 3, 2024

Abstract Background To enhance Niangya yak's reproductive efficiency and breeding process, this study employed Label-free quantitative proteomics technology to analyze fresh (F) chilled (C) semen samples.Result The analysis revealed an extensive repertoire of 18,338 peptide segments 2,579 proteins within yak semen. Among them, 2,300 were successfully annotated in the database. Notably, a total 300 DEPs identified between C F groups; comprising 129 up-regulated 171 down-regulated DEPs. GO enrichment highlighted that these may be involved lipid metabolism as well protein binding with proteases phosphorylation processes associated sperm cryodamage. KEGG showed 35 mapped pathways related motility, plasma membrane integrity apoptosis. Specifically, ribosome pathway, glycerophospholipid ferroptosis phospholipase D signaling pathway might closely linked decreased vitality, damaged integrity, apoptosis induced by freezing Based on PPI groups F, has preliminarily potential HSPBP1, HSP90AB1, CCDC83, PMSD4, ISYNA1, KLHL10, UAB1 are vitality cell caused cryodamage.Conclusion This represents first their involvement biological frozen using technology. It provides theoretical foundation for comprehending mechanism cryodamage enhancing yaks.

Язык: Английский

Процитировано

0