Metabolomic Analysis and Biochemical Profiling of Cadmium-Induced Metabolic Impairment and Its Amelioration by Resveratrol DOI Creative Commons

Kainat Ilyas,

Kanwal Rehman,

Hajra Iqbal

и другие.

Bioengineering, Год журнала: 2024, Номер 11(11), С. 1141 - 1141

Опубликована: Ноя. 13, 2024

Exposure to heavy metals, particularly cadmium (Cd), poses significant health risks because of their toxic effects and potential for bioaccumulation in living organisms. This study examined the biochemical metabolomic changes induced by Cd exposure an animal model via advanced liquid chromatography with tandem mass spectrometry (LC-MS/MS) assays reveal disruptions lipid amino acid metabolism as well alterations key metabolic pathways. led weight loss, hyperglycemia, insulin resistance, indicating its role disorders such diabetes. The accumulation liver kidneys, identified ICP-OES, corresponded elevated levels (ALT, AST) kidney (BUN, creatinine) biomarkers, suggesting organ-specific toxicity. At level, caused metabolites ceramides sphingolipids, which are associated resistance broader impairments. Amino was also significantly disrupted, increased concentrations acids phenylalanine, tryptophan, arginine affecting pathways urea cycle Krebs cycle. These disturbances linked oxidative stress, systemic inflammation, impaired glucose regulation, evidenced CRP IL-6 levels. protective resveratrol (RSV) were clearly demonstrated this study. RSV treatment ameliorated Cd-induced alterations, shown improved glycemic control, restored profiles, normalized concentrations. Additionally, reduced inflammatory markers function, highlighting antioxidant properties a therapeutic agent against However, did not reduce organs, that related mitigating damage disruption rather than promoting excretion. enhances our understanding molecular mechanisms underlying impairments highlights combating findings underscore need further research into metal mitigation protect human health, areas environmental contamination.

Язык: Английский

Astragaloside IV attenuates cadmium induced nephrotoxicity in rats by activating Nrf2 DOI Creative Commons
Yuchen Li, Jiayi Zhou, Tianxin Zhang

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 15, 2025

Acute kidney injury (AKI) has become a disease of global concern due to its high morbidity and mortality. This highlighted the need for renoprotective agents. Astragaloside IV (AS-IV) is saponin isolated from Astragalus membranaceus with good antioxidant, anti-inflammatory anti-tumor properties. In this study, HK2 cells rat model were utilized explore protective effect AS-IV against cadmium chloride-induced oxidative stress-induced apoptosis. CdCl2-induced apoptosis, ROS production, mitochondrial membrane potential alterations significantly inhibited in -treated cells. Expression mitochondria-associated apoptotic proteins Cleaved-Caspase3, Cleaved-Caspase9, Cleaved-PARP was reduced after intervention. addition, Rat weight loss also alleviated symptoms nephrotoxicity injury. Further experiments showed that suppresses heavy metal Cd-induced mitochondria-mediated apoptosis by regulating Nrf2/HO-1 pathway. conclusion, could protect metal-induced toxicity be used as nephroprotective agent.

Язык: Английский

Процитировано

0

The Preventive Impact of Chokeberry (Aronia melanocarpa L.) Extract Regarding the Disruption of Calcium and Phosphorus Homeostasis and Chosen Pathways of Its Regulation in an Animal Model of General Population Exposure to Cadmium DOI Open Access
Małgorzata M. Brzóska, Małgorzata Gałażyn-Sidorczuk, Joanna Rogalska

и другие.

Nutrients, Год журнала: 2025, Номер 17(4), С. 702 - 702

Опубликована: Фев. 16, 2025

Background: Our previous research in an experimental model of current environmental human exposure to cadmium (Cd) (female rats fed a diet containing Cd at 1 and 5 mg/kg for up 2 years) revealed that chronic treatment with this toxic element destroyed the metabolism bone tissue, decreased mineralisation, weakened biomechanical properties, whereas co-administration 0.1% chokeberry (Aronia melanocarpa L. (Michx.) Elliott berry) extract (AME) ameliorated osteotoxic action Cd. Methods: In study, it was explored whether unfavourable effect protective AME might be mediated by impact on essential elements such as calcium (Ca) inorganic phosphorus (Pi), including pathways its regulation calciotropic hormones (parathormone—PTH, calcitonin—CT, 1,25-dihydroxyvitamin D3—1,25(OH)2D3) Klotho. Results: Low-level (1 mg/kg) caused only temporary elevation serum PTH concentration decline CT. Moderate (5 body homeostasis both mineral (lowered their concentrations enhanced urinary loss), influenced Klotho hormones, well reduced 25-hydroxyvitamin D 1alpha-hydroxylase (1alpha-OHase) 1,25(OH)2D3 kidney. The application during intoxication improved involved maintaining Ca Pi allowed subjects maintain proper levels these urine. Conclusions: conclusion, low-to-moderate may exert influencing regulating destroying status minerals. It seems possible mechanism osteoprotective involves normalization improvement Pi. This study provided further evidence products effective strategy counteracting effects

Язык: Английский

Процитировано

0

Role of Anonychium africanum (Plantae, Fabaceae) in Metal Oxido-Inflammatory Response: Protection Evidence in Gonad of Male Albino Rat DOI Creative Commons

Harrison A. Ozoani,

Orish Ebere Orisakwe, Costantino Parisi

и другие.

Antioxidants, Год журнала: 2024, Номер 13(9), С. 1028 - 1028

Опубликована: Авг. 24, 2024

Male fertility is strongly affected by the overexpression of free radicals induced heavy metals. The aim this study was to evaluate potential antioxidant, anti-inflammatory, and gonado-protective effects natural compounds. Biochemical morphological assays were performed on male albino rats divided into five groups: a control group (water only), orally exposed metal mixture Pb-Cd-Hg-As alone three groups co-administered an aqueous extract Nigerian medicinal plant, Anonychium africanum (Prosopis africana, PA), at different concentrations (500, 1000, 1500 mg/kg) for 60 days. significant rise in testicular weight, bioaccumulation, oxidative stress, pro-inflammatory apoptotic markers, while semen analysis indicated lower viability decrease normal sperm count, plasma reproductive hormones showed variation. Parallel phytochemical investigations that PA has bioactive compounds like phlobatannins, flavonoids, polyphenols, tannins, saponins, steroids, alkaloids, which are protective against injury neural tissues. Indeed, presence with mitigated toxic metals’ impact, determined observing oxido-inflammatory response via nuclear factor erythroid 2-related 2, thus boosting health.

Язык: Английский

Процитировано

1

Metabolomic Analysis and Biochemical Profiling of Cadmium-Induced Metabolic Impairment and Its Amelioration by Resveratrol DOI Creative Commons

Kainat Ilyas,

Kanwal Rehman,

Hajra Iqbal

и другие.

Bioengineering, Год журнала: 2024, Номер 11(11), С. 1141 - 1141

Опубликована: Ноя. 13, 2024

Exposure to heavy metals, particularly cadmium (Cd), poses significant health risks because of their toxic effects and potential for bioaccumulation in living organisms. This study examined the biochemical metabolomic changes induced by Cd exposure an animal model via advanced liquid chromatography with tandem mass spectrometry (LC-MS/MS) assays reveal disruptions lipid amino acid metabolism as well alterations key metabolic pathways. led weight loss, hyperglycemia, insulin resistance, indicating its role disorders such diabetes. The accumulation liver kidneys, identified ICP-OES, corresponded elevated levels (ALT, AST) kidney (BUN, creatinine) biomarkers, suggesting organ-specific toxicity. At level, caused metabolites ceramides sphingolipids, which are associated resistance broader impairments. Amino was also significantly disrupted, increased concentrations acids phenylalanine, tryptophan, arginine affecting pathways urea cycle Krebs cycle. These disturbances linked oxidative stress, systemic inflammation, impaired glucose regulation, evidenced CRP IL-6 levels. protective resveratrol (RSV) were clearly demonstrated this study. RSV treatment ameliorated Cd-induced alterations, shown improved glycemic control, restored profiles, normalized concentrations. Additionally, reduced inflammatory markers function, highlighting antioxidant properties a therapeutic agent against However, did not reduce organs, that related mitigating damage disruption rather than promoting excretion. enhances our understanding molecular mechanisms underlying impairments highlights combating findings underscore need further research into metal mitigation protect human health, areas environmental contamination.

Язык: Английский

Процитировано

0