Enhanced Salivary and General Oxidative Stress in Hashimoto’s Thyroiditis Women in Euthyreosis

Journal of Clinical Medicine, Год журнала: 2020, Номер 9(7), С. 2102 - 2102

Опубликована: Июль 3, 2020

Hashimoto's thyroiditis (HT) is one of the most common autoimmune diseases. Although HT inextricably linked to oxidative stress, there have been no studies assessing salivary redox homeostasis or gland function in patients with HT. This study first compare antioxidant defense and stress biomarkers non-stimulated (NWS) stimulated (SWS) whole saliva plasma/erythrocytes compared controls. The included 45 women euthyreosis period as well an age- gender-matched control group. We showed that NWS secretion was significantly lower healthy controls, similar amylase activity SWS. Catalase peroxidase activities were considerably higher SWS patients, while concentrations reduced glutathione uric acid comparison subjects. Total potential lower, total oxidant status level oxidation products proteins (advanced glycation end products, advanced protein products) lipids (malondialdehyde, lipid hydroperoxides) NWS, plasma patients. In conclusion, both glands euthyreosis, ability maintain hindered. we observed damage lipids; thus, some may present a diagnostic value.

Язык: Английский

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Activity of enzymatic antioxidants in periodontitis: A systematic overview of the literature

Dental and Medical Problems, Год журнала: 2019, Номер 56(4), С. 419 - 426

Опубликована: Дек. 30, 2019

Periodontitis is initiated by a bacterial infection and an abnormal immune response of the host resulting in formation dysbiotic subgingival biofilm progressive destruction attachment apparatus teeth.It believed that disturbances local and/or general indicators oxidative stress are one mechanisms etiopathogenesis periodontitis.Organisms using oxygen their metabolic processes equipped with protect against activity oxygen-free radicals.They commonly referred to as 'the antioxidative barrier system' .The main enzymatic antioxidants which have been widely studied gingival fluid, saliva blood serum patients periodontitis superoxide dismutase (SOD), glutathione peroxidase (GPx) catalase (CAT).The aim this systematic overview literature was present current research on these antioxidant enzymes periodontitis.Findings tissue, saliva, course types classified so far (chronic or aggressive) quite disparate.Their tissue usually elevated, whereas it reduced.These differences may resulted from different methods biochemically assessing activity, not reflected stage risk progression periodontitis.

Язык: Английский

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Bariatric Surgery Normalizes Protein Glycoxidation and Nitrosative Stress in Morbidly Obese Patients

Antioxidants, Год журнала: 2020, Номер 9(11), С. 1087 - 1087

Опубликована: Ноя. 4, 2020

The results of recent studies indicate the key role nitrosative stress and protein oxidative damage in development morbid obesity. Nevertheless, effect bariatric surgery on oxidation/glycation nitrosative/nitrative is not yet known. This first study evaluating glycoxidation morbidly obese patients before after (one, three, six twelve months) laparoscopic sleeve gastrectomy. included 50 women with obesity as well age- gender-matched healthy controls. We demonstrated significant increases serum myeloperoxidase, plasma glycooxidative products (dityrosine, kynurenine, N-formyl-kynurenine, amyloid, Amadori products, glycophore), (ischemia modified albumin) (nitric oxide, peroxy-nitrite, S-nitrosothiols nitro-tyrosine) subjects compared to lean controls, whereas tryptophan total thiols were statistically decreased. Bariatric generally reduces abnormalities proteins stress. Noteworthily, metabolic syndrome (MS+), we showed no differences most redox biomarkers, without MS (MS-). However, two markers: able differentiate MS+ MS- high specificity sensitivity: peroxy-nitrite (>70%) (>60%). Further are required confirm diagnostic usefulness such biomarkers.

Язык: Английский

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Effects of age and gender on the redox homeostasis of morbidly obese people

Free Radical Biology and Medicine, Год журнала: 2021, Номер 175, С. 108 - 120

Опубликована: Авг. 11, 2021

Obesity is a chronic disease of complex etiology. Recent evidence suggests that obesity caused by inflammation adipose tissue leading to metabolic disorders, cardiovascular and cancer. This the first study evaluated effects age gender on redox homeostasis, glutathione metabolism, oxidative damage plasma/serum lipids proteins in morbidly obese patients. The included 120 (60 men 60 women) patients with class 3 (BMI > 40 kg/m2), classified into three groups depending age: 20–39 years (n = 20), 40–59 20) or older 20). number was calculated priori based our previous experiment. We observed reduction serum activity antioxidant enzymes (↓SOD) plasma concentration non-enzymatic antioxidants (↓GSH) compared lean controls, which further decreased age. Redox status (↑TAC, ↑TOS ↓OSI) women shifted towards oxidation. Moreover, lipid (↑MDA ↑LOOH) protein (↑AOPP, ↑AGE ↑Amadori products) products oxidation nitrosylation/nitration (↑total NO, ↑S-nitrosothiols, ↑peroxynitrite ↑nitrotyrosine) were elevated both genders higher elderly. Interestingly, concentrations nitrosative stress markers generally at same Summarizing, we showed total antioxidant/oxidant potential significantly increased Obese have oxidation, glycation nitration as controls. Disturbances homeostasis increase Oxidative are more intense than

Язык: Английский

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Characteristics of HIF-1Α and HSP70 MRNA Expression, Level, and Interleukins in Experimental Chronic Generalized Periodontitis

MicroRNA, Год журнала: 2024, Номер 13(2), С. 132 - 139

Опубликована: Апрель 15, 2024

Objectives: Periodontal diseases are a rather complex problem of modern dentistry and do not have only medical but also social significance. : The objective this study is to weigh the effect mixture Thiotriazoline L-arginine (1:4) on parameters system endogenous cytoprotection blood periodontal illness in rats with experimental chronic generalized periodontitis substantiate further blend. Materials Methods: aimed evaluate impact combination (in ratio 1:4) periodontium periodontitis. A group outbred weighing 190-220 g sourced from vivarium Institute Pharmacology Toxicology Academy Medical Sciences Ukraine were divided into four groups, each consisting 10 animals. (1) Intact group, animals that injected intragastrically solution sodium chloride 0.9% for 30 days. (2) control, CGP who (3) intramuscularly + dosage 200 mg/kg (30 days). (4) CGP, which daily intragastric reference drug Mexidol, 250 In study, we utilized two substances: hydrochloride. was prepared at Department Pharmaceutical Chemistry ZSMU. At conclusion experiment, carefully removed while under thiopental-sodium anesthesia, administered 40 mg/kg. Results: We found administration combined preparation leads significant decrease concentration pro-inflammatory cytokines IL-1b TNF-a by 56.1% 71%, respectively. Conclusion: Mexidol mg/kg, as well Larginine 1:4 resulted reduction gingival pocket depth CGP. Specifically, reduced 6 mm (p < 0.05) 4 L-arginine. Additionally, exhibited minimal bleeding, swelling, tooth mobility when treated noteworthy TNF-a. there 71% levels. course (200 mg/kg) led an increased expression HSP70 mRNA 8.2 times HIF-1a times. 2.8 against background increase 95% 0.05). Also, c-Fos 36.7% compared control group.

Язык: Английский

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Gingival Crevicular Fluid Peptidome Profiling in Healthy and in Periodontal Diseases

International Journal of Molecular Sciences, Год журнала: 2020, Номер 21(15), С. 5270 - 5270

Опубликована: Июль 24, 2020

Given its intrinsic nature, gingival crevicular fluid (GCF) is an attractive source for the discovery of novel biomarkers periodontal diseases. GCF contains antimicrobial peptides and small proteins which could play a role in specific immune-inflammatory responses to guarantee healthy status prevent Presently, several proteomics studies have been performed leading increased coverage proteome, however fewer efforts done explore natural peptides. To fill such gap, this review provides overview mass spectrometric platforms experimental designs aimed at peptidome profiling, including our own data experiences gathered from over years matrix-assisted laser desorption ionization/time flight spectrometry (MALDI-TOF MS) based approach field. These tools might be useful capturing snapshots containing diagnostic clinical information on individual population scale, may used as code not only diagnosis nature or stage inflammatory process disease, but more importantly, prognosis, still unmet medical need. As matter fact, current peptidomics investigations suffer lack standardized procedures, posing serious problem interpretation. Descriptions address concerns will highlighted.

Язык: Английский

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Phloroglucinol Strengthens the Antioxidant Barrier and Reduces Oxidative/Nitrosative Stress in Nonalcoholic Fatty Liver Disease (NAFLD)

Oxidative Medicine and Cellular Longevity, Год журнала: 2021, Номер 2021(1)

Опубликована: Янв. 1, 2021

Nonalcoholic fatty liver disease (NAFLD) is one of the most commonly occurring diseases within western dietary patterns. Usually untreated, it may lead to type 2 diabetes mellitus (T2DM), steatohepatitis (NASH), and hepatocellular carcinoma (HCC). Besides its severe aftermath, up now, there no known therapeutic approach this in everyday clinical practice. Most NAFLD patients are encouraged do physical activities or diet change remain without pharmacological treatment. In study, we present phloroglucinol (PHG) as a novel promising compound PHG significantly increased level enzymatic nonenzymatic antioxidants both palmitate hydrogen peroxide‐induced oxidative stress models. Strengthened antioxidative defense reduced oxidative/nitrosative damage cell proteins, lipids, carbohydrates. Furthermore, treatment hepatic steatosis; lowered inflammatory markers, such NF‐ κ B HIF‐1 α ; inhibited apoptosis. Moreover, had more comprehensive effect than other used antioxidants: N‐acetylcysteine (NAC) ‐lipoic acid (ALA), suggesting usability. Therefore, our paper supports benefits natural compounds therapeutical NAFLD.

Язык: Английский

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Impact of Circulating Cell-Free DNA (cfDNA) as a Biomarker of the Development and Evolution of Periodontitis

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(12), С. 9981 - 9981

Опубликована: Июнь 10, 2023

In the last few decades, circulating cell-free DNA (cfDNA) has been shown to have an important role in cell apoptosis or necrosis, including development and evolution of several tumors inflammatory diseases humans. this regard, periodontitis, a chronic disease that can induce destruction supporting components teeth, could represent stimulus linked various range systemic diseases. Recently, possible correlation between periodontal cfDNA shown, representing new diagnostic-therapeutic perspectives. During is released biological fluids such as blood, saliva, urine other body represents index inflammation. Due possibility withdrawing some these liquids non-invasive way, be used biomarker for disease. addition, discovering proportional relationship levels severity expressed through extent, open prospect using therapeutic target. The aim article report what researchers discovered recent years about development, therapy periodontitis. analyzed literature review shows considerable potential diagnostic, target disease; however, further studies are needed clinical practice.

Язык: Английский

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Current clinical framework on nitric oxide role in periodontal disease and blood pressure

Clinical Oral Investigations, Год журнала: 2024, Номер 28(10)

Опубликована: Сен. 12, 2024

Язык: Английский

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Salivary Gland Dysfunction in Patients with Chronic Heart Failure Is Aggravated by Nitrosative Stress, as Well as Oxidation and Glycation of Proteins

Biomolecules, Год журнала: 2021, Номер 11(1), С. 119 - 119

Опубликована: Янв. 18, 2021

Chronic heart failure (HF) is an important clinical, social, and economic problem. A key role in HF progression played by oxidative stress. Free oxygen radicals, formed under the conditions of hypoxia reperfusion, participate myocardial stunning other forms post-reperfusion damage. patients also suffer from disorders connected with saliva secretion. However, still little known about mechanisms that impair secretory function salivary glands these patients. In presented study, we were first to compare antioxidant barrier, protein glycoxidation, nitrosative/nitrative stress non-stimulated (non-stimulated whole (NWS)) stimulated (SWS) The study included 50 normal (NS) secretion (n = 27) hyposalivation (HS) 23), as well age- gender-matched control group 50). We demonstrated that, NWS HS, concentration low-molecular-weight non-enzymatic antioxidants decreased (↓total polyphenols, ↓ascorbic acid, ↓reduced glutathione, ↓albumin) compared secretion, (except albumin). observed increased content glycoxidation products (↑dityrosine, ↑kynurenine, ↑glycophore) SWS HS healthy controls. Interestingly, dityrosine, N-formylkynurenine, glycophore was significantly higher those NS NO considerably lower, while levels peroxynitrite nitrotyrosine subjects Salivary gland dysfunction occurs chronic submandibular being least efficient. Oxidative/nitrosative may be one responsible for impairment

Язык: Английский

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