ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
17(1), С. 1167 - 1178
Опубликована: Дек. 23, 2024
A
biofuel
cell
is
an
electrochemical
device
using
exoelectrogen
or
biocatalysts
to
transfer
electrons
from
redox
reactions
the
electrodes.
While
wild-type
microbes
and
natural
enzymes
are
often
employed
as
biocatalysts,
genetically
engineered
modified
organisms
have
been
developed
enhance
activity.
Here,
we
demonstrated
a
redox-enzyme
integrated
microbial
fuel
(REI-MFC)
design
based
on
exoelectrogen-enhancing
strategy
that
reinforces
electrogenic
activity
of
Shewanella
oneidensis
MR1
by
displaying
extra
enzyme
surface.
We
constructed
cell-surface
display
system
for
MR-1
porting
autotransporter
Escherichia
coli
into
strain.
The
functionality
was
validated
examining
various
displayed
surface
S.
MR-1.
implementation
REI-MFC
accomplished
strain
originating
swine
NADH-cytochrome
b5
reductase
3
(B5R3).
At
polarization
test
enhanced
in
operating
MFC
environment,
current
generation
(ΔIa,
peak:
10.4
±
1.9
μA)
B5R3
4.7-fold
higher
than
(2.2
0.3
μA).
maximum
charge
resistance
(Rct)
under
optimized
conditions
70%
lower
exploited
this
study
facilitated
design.
ACS ES&T Engineering,
Год журнала:
2024,
Номер
4(7), С. 1520 - 1539
Опубликована: Июнь 20, 2024
Extracellular
electron
transport
(EET)
is
a
biological
process
where
microorganisms
can
donate
electrons
from
the
interior
of
their
cells
to
external
acceptors
or
act
as
receive
sources
and
electrodes.
This
often
occurs
in
surrounding
environment
within
biofilms,
enabling
redox
reactions
essential
for
energy
metabolism.
review
evaluates
latest
developments
transfer
research
environmental
biotechnology,
showcasing
its
varied
applications
across
bioelectrochemical
systems
(BES),
including
microbial
fuel
electrosynthesis
CO2
upcycling,
well
utilization
non-BES
such
anaerobic
digestion
bioleaching
useful
resource
recovery.
The
emphasizes
interdisciplinary
approach
EET
research,
merging
microbiology,
chemistry,
engineering,
material
science,
system
control
engineering.
paper
provides
insights
into
performance
optimization
outlook
future
industrial
commercial
applications.
also
explores
potential
mitigate
global
challenges,
offering
innovative
biotechnological
solutions
that
pave
way
sustainable
circular
bioeconomy.
Annals of Microbiology,
Год журнала:
2024,
Номер
74(1)
Опубликована: Июнь 3, 2024
Abstract
Microbial
fuel
cells
(MFCs),
which
use
bacterial
electron
transport
mechanisms
to
generate
energy,
have
become
a
viable
technology
for
renewable
energy
production.
This
review
investigates
the
evolutionary
and
functional
connections
between
transduction
mitochondrial
chains,
building
on
endosymbiont
theory
of
eukaryotic
cell
evolution.
The
conserved
features
similarities
prokaryotic
pathways
were
elucidated,
highlighting
their
common
origins
roles
in
cellular
bioenergetics.
discussion
explores
essential
governing
movement
electrons
ions
across
biological
membranes,
crucial
generating
maintaining
electrochemical
gradients
bacteria
mitochondria.
Capitalizing
these
insights,
we
explore
applications
electrogenic
MFCs
electricity
generation.
Optimal
conditions
enhancing
transfer
electrode
surfaces
are
identified,
paving
way
improved
MFC
performance.
Potential
large-scale
implementations
wastewater
treatment,
biosensing,
bioremediation
contaminated
environments
discussed,
underscoring
versatility
environmental
benefits.
importance
investigating
bioenergetic
at
both
molecular
scales
fully
harnessing
capabilities
microbial
conversion
systems
is
highlighted
this
review.
By
bridging
gap
fundamental
processes
sustainable
technologies,
aim
advance
solutions
that
harness
remarkable
microorganisms.
