Опубликована: Янв. 1, 2025
Язык: Английский
International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(1), С. 355 - 355
Опубликована: Янв. 3, 2025
The widespread use of disinfectants and antiseptics has led to the emergence nosocomial pathogens that are less sensitive these agents, which in combination with multidrug resistance (MDR) can pose a significant epidemiologic risk. We investigated susceptibility Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus 0.05% chlorhexidine (CHX) solution biocidal S7 composite based on CHX (0.07%) benzalkonium chloride (BAC, 0.055%). prevalence efflux pump genes associated biocide their relationship antibiotic was also determined. Both biocides were more effective against Gram-positive S. than Gram-negative bacteria. most resistant strains P. aeruginosa strains, mainly killed by 0.0016% 0.0000084% (CHX)/0.0000066% (BAC) S7. bactericidal effect observed after 10 min, while only 30 min. qacEΔ1 qacE prevalent among E. coli K. mexB often detected aeruginosa. norA, norB, mepA, mdeA, sepA aureus. highlights potential problem whereby sensitivity bacteria could decline rapidly future.
Язык: Английский
Процитировано
0
Microbiology Research, Год журнала: 2025, Номер 16(1), С. 16 - 16
Опубликована: Янв. 12, 2025
Chlorhexidine and cetrimide are often used as antiseptics disinfectants. While their individual activities well-documented, synergism has rarely been evaluated. Here, we attempted to evaluate the antimicrobial antibiofilm effects of combination these two agents against environment isolates, viz., P. aeruginosa S. aureus. The was assayed by determining fractional inhibitory concentrations, while were determined using crystal violet staining resazurin assay. Further, on biofilms visualized brightfield confocal laser scanning microscopy. Our results show that antimicrobials resulted in synergistic inhibition growth. When tested at concentrations below MICs (one-quarter MICs), able significantly reduce adherence aureus a polystyrene surface, no effect observed for aeruginosa. also viability pre-formed both bacteria, thereby showing its potential. Next, evaluated performance this wide array micro-organisms. This fixed-dose formulation exhibited significant reduction an clinically relevant micro-organisms, including ESKAPE pathogens, Mycobacterium sp., MRSA, Leptospira, Candida norovirus adenovirus. Overall, it can be inferred chlorhexidine is potential biocide continues use antisepsis disinfection infection-causing pathogens.
Язык: Английский
Процитировано
0
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Microorganisms, Год журнала: 2025, Номер 13(2), С. 324 - 324
Опубликована: Фев. 2, 2025
Marine bacteria that have adapted to thrive in extreme environments, such as Pseudoalteromonas haloplanktis TAC125 (PhTAC125), offer a unique biotechnological potential. The discovery of an endogenous megaplasmid (pMEGA) raises questions about its metabolic impact and functional role strain. This study aimed at streamlining the host genetic background by curing PhTAC125 pMEGA plasmid using sequential approach. We combined homologous recombination exploiting suicide vector, with PTasRNA gene-silencing technology interfering replication machinery. approach led construction novel KrPL2 strain, cured plasmid, which exhibited no significant differences growth behavior, though showcasing enhanced resistance oxidative stress reduced capacity for biofilm formation. These findings represent achievement developing our understanding applications recombinant protein production. opens up possibility valuable elements further advancing tools PhTAC125.
Язык: Английский
Процитировано
0
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0