This
study
evaluated
the
influence
of
methotrexate
(MTX)
and
non-surgical
periodontal
treatment
(NSPT)
on
composition
oral-gut
microbiota
in
patients
with
rheumatoid
arthritis
(RA).
Assessments
were
performed
at
baseline
(T0),
6
months
after
MTX
(T1),
45
days
NSPT
(T2).
The
oral
gut
was
assessed
by
amplifying
V4
region
16S
gene
from
subgingival
plaques
stools.
results
analysis
continuous
variables
presented
descriptively
non-parametric
tests
adopted.
Thirty-seven
(27
periodontitis)
T0;
32
(24
T1;
28
(17
T2.
Nine
individuals
lost
to
follow-up.
tended
reduce
alpha
diversity
microbiota,
while
appeared
increase
number
different
species
microbiota.
influenced
beta
relative
abundance
directly
status.
did
not
affect
condition
but
modified
interactions
between
clinical
parameters
affected
richness
However,
parameters.
Journal of Dental Sciences,
Год журнала:
2023,
Номер
18(4), С. 1453 - 1466
Опубликована: Июль 11, 2023
Oral
infection
is
a
common
clinical
symptom.
While
antibiotics
are
widely
employed
as
the
primary
treatment
for
oral
diseases,
emergence
of
drug-resistant
bacteria
has
necessitated
exploration
alternative
therapeutic
approaches.
One
such
modality
antimicrobial
photodynamic
therapy
(aPDT),
which
utilizes
light
and
photosensitizers.
Indeed,
aPDT
been
used
alone
or
in
combination
with
other
options
dealing
periodontal
disease
elimination
biofilms
from
bacterial
community
to
achieve
bone
formation
and/or
tissue
regeneration.
In
this
review
article,
addition
factors
affecting
efficacy
aPDT,
various
photosensitizers,
latest
technology
perspectives
on
discussed
detail.
More
importantly,
article
emphasizes
novel
design
applications
well
synergistic
effects
chemical
biomolecules
complete
eradication
even
enhance
biological
performance
tissues
surrounding
treated
area.
Photobiomodulation Photomedicine and Laser Surgery,
Год журнала:
2025,
Номер
43(3), С. 103 - 113
Опубликована: Фев. 12, 2025
Objectives:
This
split-mouth
randomized
controlled
clinical
trial
aimed
to
evaluate
the
efficacy
of
diode
laser
therapy
as
an
adjunct
scaling
and
root
planning
(SRP)
in
management
patients
with
stage
II
grade
A
periodontitis.
Methods:
Thirty
participants
were
randomly
assigned
receive
either
SRP
alone
(control
group)
or
application
(study
on
contralateral
side.
Clinical
parameters
(plaque
index,
gingival
probing
depth,
attachment
level)
microbiological
markers;
Aggregatibacter
actinomycetemcomitans
(A
actinomycetemcomitans),
Porphyromonas
gingivalis
(P
gingivalis),
Fusobacterium
nucleatum
(F
nucleatum)
evaluated
at
baseline
4,
8,
12
weeks
post-treatment.
Results:
Both
groups
reported
significant
improvements
all
over
follow-up
period.
The
study
group
a
significantly
higher
reduction
depth
than
SRP-alone
group.
Microbiological
evaluation
revealed
greater
reductions
P
F.
levels
later
time
points,
whereas
no
alterations
observed
for
actinomycetemcomitans.
Conclusions:
Our
results
suggest
that
adjunctive
use
may
improve
findings
nonsurgical
periodontal
Diagnostics,
Год журнала:
2025,
Номер
15(5), С. 609 - 609
Опубликована: Март 3, 2025
Background:
Diabetes
mellitus
(DM)
is
a
major
risk
factor
for
the
development
of
periodontal
disease
and
aggravates
severity
conditions.
Matrix
metalloproteinases
(MMPs)
are
known
to
degrade
ligament
attachment
bone
matrix
proteins.
Increased
expression
CD147
associated
with
increased
synthesis
several
MMPs,
being
modulator
MMP
expression,
including
that
MMP-14.
The
purpose
this
study
was
quantify
compare
expressions
MMP-14
in
gingival
tissues
patients
without
type
2
diabetes
mellitus.
Material
Methods:
In
histological
study,
we
included
33
subjects
disease:
16
DM
(test
group)
17
systemically
healthy
(control
group).
Tissue
fragments
were
processed
using
an
immunohistochemistry
technique
determine
immunoreactivity
(IR)
intensity
CD147.
Results:
group
periodontitis,
56.2%
showed
weak
positive
(+),
while
43.8%
had
intensely
(+++)
Statistically
significant
differences
between
test
control
groups
(p
=
0.004,
p
0.883,
0.002)
found
membranous
IR
moderate
(++)
CD
147,
(+++).
0.001,
0.002,
0.003)
Conclusions:
significantly
higher
147
demonstrates
role
these
biomarkers
pathology
patients.
It
can
be
assumed
could
further
investigated
as
potential
predictive
biomarkers.
Applied Sciences,
Год журнала:
2025,
Номер
15(8), С. 4504 - 4504
Опубликована: Апрель 19, 2025
The
purpose
of
this
study
was
to
evaluate
the
bactericidal
effect
270
nm
UV-C
light-emitting
diode
(LED)
light
delivered
through
a
newly
designed
prototype
device
with
thin
optical
fiber
against
Enterococcus
faecalis
(E.
faecalis).
device,
developed
integrate
into
optic
(diameter
124
µm)
connected
LED
(Luminous
Device;
Sunnyvale,
CA,
USA)
via
specialized
double-lens
system
that
focuses
divergent
achieve
65
mm
working
distance
and
numerical
aperture
0.22.
E.
faecalis,
cultured
at
37
°C
under
aerobic
conditions
for
24
h.
positioned
10
above
bacterial
culture
prepared
in
wells
96-well
plate.
cells
were
exposed
irradiation
0,
10,
30,
60,
90,
120
180
s.
Following
irradiation,
OD600
values
measured
after
incubation
an
additional
data
statistically
analyzed
using
one-way
ANOVA,
followed
by
Tukey’s
honestly
significant
difference
(HSD)
test
significance
level
0.05.
UV
significantly
reduced
growth
time-dependent
manner
(p
<
0.05).
No
changes
observed
0
s,
while
peak
reductions
occurred
effects
beginning
30
s
increasing
over
time.
wavelength
highly
effective
action
faecalis.
custom-designed
delivery
effectively
integrated
source
fiber,
allowing
efficient
transmission
demonstrating
its
potential
application
narrow
spaces
such
as
root
canals.
Frontiers in Oral Health,
Год журнала:
2024,
Номер
5
Опубликована: Май 30, 2024
Chronic
periodontitis
is
a
ubiquitous
inflammatory
disease
in
dental
healthcare
that
challenging
to
treat
due
its
impact
on
bone
and
tooth
loss.
Conventional
mechanical
debridement
has
been
eliminating
complex
subgingival
biofilms.
Hence,
adjunctive
approaches
like
low-level
laser
antimicrobial
photodynamic
therapy
(A-PDT)
utilising
methylene
blue
(MB)
have
emerging
recent
times.
This
review
evaluates
the
latest
research
use
of
MB-mediated
A-PDT
decrease
microbial
count
enhance
clinical
results
chronic
periodontitis.
Studies
shown
interaction
between
light
MB
generates
phototoxic
effect
thereby,
pathogenic
bacteria
within
periodontal
pockets.
Moreover,
numerous
trials
using
can
reduce
probing
depths,
improve
attachment
levels,
bleeding
during
comparison
traditional
treatment
approaches.
Notably,
shows
superior
antibiotic
resistance
compared
conventional
treatments.
In
conclusion,
promise
as
an
for
Additional
required
standardize
protocols
assess
long-term
outcomes
with
Diagnostics,
Год журнала:
2024,
Номер
14(10), С. 1054 - 1054
Опубликована: Май 19, 2024
the
aim
of
study
was
to
assess
microbiological
air
quality
in
operating
theatres
by
determining
level
contamination
and
critical
surfaces
using
passive
sampling
method
compliance
theatre
staff
with
infection
control
measures.
Microorganisms,
Год журнала:
2023,
Номер
12(1), С. 68 - 68
Опубликована: Дек. 29, 2023
This
study
evaluated
the
changes
in
composition
of
oral–gut
microbiota
patients
with
rheumatoid
arthritis
(RA)
caused
by
methotrexate
(MTX)
and
non-surgical
periodontal
treatment
(NSPT).
Assessments
were
performed
at
baseline
(T0),
6
months
after
MTX
(T1),
45
days
NSPT
(T2).
The
oral
gut
was
assessed
amplifying
V4
region
16S
gene
from
subgingival
plaques
stools.
results
analysis
continuous
variables
presented
descriptively
non-parametric
tests
Spearman’s
correlation
adopted.
A
total
37
(27
periodontitis)
T0;
32
(24
T1;
28
(17
T2.
tended
to
reduce
alpha
diversity
microbiota,
while
appeared
increase
number
different
species
microbiota.
influenced
beta
relative
abundance
directly
status.
did
not
affect
condition
but
modified
correlations
that
varied
weak
moderate
(p
<
0.05)
between
clinical
parameters
affected
richness
However,
influence
parameters.
Encyclopedia,
Год журнала:
2023,
Номер
3(2), С. 458 - 467
Опубликована: Апрель 5, 2023
This
entry
explores
the
innovative
use
of
lasers
in
non-surgical
treatment
periodontitis,
shedding
light
on
advantages,
effectiveness,
and
limitations
this
approach.
There
has
been
a
massive
eruption
innovations
technologies
to
assist
periodontal
diseases
over
past
30
years.
The
opened
new
horizons
possibilities
that
can
enhance
treatments.
However,
their
is
not
always
based
validated
concepts
evidence-based
protocols.
Hence,
aims
describe,
summarize,
assess
available
evidence
current
laser-assisted
protocols
periodontitis.
Four
distinct
approaches
are
addressed:
(1)
for
removal
subgingival
calculus,
(2)
photodynamic
therapy/photoactivated
disinfection,
(3)
photobiomodulation
therapy,
(4)
high-power
lasers.
Based
studies
guidelines
recommendations,
exhibits
several
such
as
increased
disinfection
pockets
junctional
epithelium
connective
tissue,
calculus
biofilm,
bactericidal
effect
pathogens.
Moreover,
therapy
seems
play
positive
role
management
inflammatory
process
Although
promising,
periodontitis
needs
be
further
investigated.
Medicina,
Год журнала:
2024,
Номер
60(3), С. 437 - 437
Опубликована: Март 6, 2024
Background
and
Objectives:
This
paper
aims
to
assess
the
role
of
laser
therapy
in
periodontitis
through
an
innovative
approach
involving
computational
prediction
advanced
modeling
performed
network
analysis
(Gaussian
graphical
models—GGMs)
structural
equations
(SEM).
Materials
Methods:
Forty
patients,
exhibiting
periodontal
pockets
with
a
minimum
depth
5
mm,
were
randomly
divided
into
two
groups:
control
group
group.
Four
specific
indicators
measured
for
each
tooth,
namely
pocket
(PPD),
clinical
attachment
level
(CAL),
bleeding
on
probing
(BOP),
plaque
index
(PI),
mean
six
values
was
recorded
at
five
time
markers
(baseline,
6
months,
1
year,
2
years,
4
years).
The
assessment
algorithm
included
enrollment,
measurements,
differential
non-surgical
treatment,
according
allocation.
Scaling,
root
planing,
chlorhexidine
1%
conducted
group,
scaling,
planing
erbium,
chromium:yttrium-scandium-gallium-garnet
(Er,CR:YSGG)
Results:
main
results
highlight
that
addition
treatment
scaling
led
notable
improvements,
decreasing
PPD
values,
reducing
BOP
scores,
increasing
CAL.
Conclusions:
Notable
relationships
between
considered
highlighted
by
both
GGMs
SEM,
thus
confirming
their
suitability
as
proxies
success
treatment.
