Molecules,
Год журнала:
2023,
Номер
28(18), С. 6654 - 6654
Опубликована: Сен. 16, 2023
Sodium
chloride
(NaCl)
is
a
commonly
used
additive
in
minimally
processed
fish-based
products.
The
addition
of
NaCl
to
fish
products
and
packaging
modified
atmosphere
usually
efficient
with
regard
limiting
the
occurrence
aquatic
environmental
pathogen
Pseudomonas
aeruginosa.
Given
negative
effects
excess
diet,
there
growing
demand
reduce
food
safer
substituents,
but
knowledge
their
impact
on
antibiotic
resistant
P.
aeruginosa
limited.
This
study
aimed
evaluate
physiological
transcriptome
characteristics
NT06
isolated
from
determine
effect
selected
concentrations
alternative
compounds
(KCl/NaL/NaC)
virulence
phenotype
genotype.
In
study,
among
microorganisms,
showed
highest
resistance
(to
ampicillin,
ceftriaxone,
nalidixic
acid,
norfloxacin)
ability
grow
at
4
°C.
Comprehensive
Antibiotic
Resistance
Database
(CARD)
Virulence
Factor
(VFDB)
revealed
presence
24
134
gene
assigned
AMR
VF
transcriptome,
respectively.
KCl,
KCl/NaL
KCl/NaL/NaC
inhibited
pyocyanin
biosynthesis,
elastase
activity,
protease
activity
40
77%.
above
phenotypic
observations
were
confirmed
via
RT-qPCR
analyses,
which
that
all
tested
genes
most
downregulated
due
treatment.
conclusion,
this
provides
insight
into
potential
foodborne
possible
impairment
those
features
by
NaL,
NaC,
exert
synergistic
can
be
Biomedicines,
Год журнала:
2023,
Номер
11(5), С. 1442 - 1442
Опубликована: Май 14, 2023
The
repurposing
of
drugs
is
one
the
most
competent
strategies
for
discovering
new
antimicrobial
agents.
Vildagliptin
a
dipeptidyl
peptidase-4
inhibitor
(DPI-4)
that
used
effectively
in
combination
with
metformin
to
control
blood
glucose
levels
diabetic
patients.
This
study
was
designed
evaluate
anti-virulence
activities
this
against
clinically
important
pathogens,
Pseudomonas
aeruginosa.
current
findings
show
significant
ability
vildagliptin-metformin
diminish
biofilm
formation,
bacterial
motility,
and
production
virulent
extracellular
enzymes
pyocyanin
pigment.
Furthermore,
drug
significantly
increased
susceptibility
P.
aeruginosa
oxidative
stress,
indicating
immunity
enhancement
eradication
cells.
In
compliance
vitro
findings,
histopathological
photomicrographs
mice
showed
considerable
protective
effect
metformin-vildagliptin
aeruginosa,
revealing
relief
inflammation
due
aeruginosa-induced
pathogenesis.
mainly
employs
quorum
sensing
(QS)
systems
its
huge
arsenal
virulence
factors.
can
be
interrupted
by
anti-QS
both
vildagliptin,
as
exhibited
affinity
QS
receptors.
Additionally,
downregulated
expression
main
three
QS-encoding
genes
These
at
very
low
concentrations
(10,
1.25
mg/mL,
respectively)
compared
(850,
50
diabetes.
Saudi Pharmaceutical Journal,
Год журнала:
2024,
Номер
32(5), С. 102041 - 102041
Опубликована: Март 18, 2024
The
rise
of
antibiotic
resistance
in
bacteria
is
becoming
a
global
concern,
particularly
due
to
the
dwindling
supply
new
antibiotics.
This
situation
mandates
discovery
antimicrobial
candidates.
Plant-derived
natural
compounds
have
historically
played
crucial
role
development
antibiotics,
serving
as
rich
source
substances
possessing
properties.
Numerous
studies
supported
reputation
6-gingerol,
prominent
compound
found
ginger
family,
for
its
antibacterial
In
this
study,
activities
6-gingerol
were
evaluated
against
Gram-negative
bacteria,
Acinetobacter
baumannii
and
Klebsiella
pneumoniae,
with
particular
focus
on
clinically
significant
Pseudomonas
aeruginosa
Gram-positive
Staphylococcus
aureus.
Furthermore,
anti-virulence
assessed
vitro,
vivo,
silico.
current
findings
showed
that
6-gingerol's
activity
effect
disruption
bacterial
cell
membrane
efflux
pumps,
it
significantly
decreased
disrupted
S.
aureus
P.
aeruginosa.
biofilm
formation
production
virulence
factors
concentrations
below
MICs.
properties
could
be
attributed
capacity
disrupt
virulence-regulating
systems;
quorum
sensing
(QS).
6-Gingerol
was
interact
QS
receptors
downregulate
genes
responsible
QS.
addition,
molecular
docking,
dynamics
(MD)
simulation
results
indicated
comparable
binding
affinity
co-crystalized
ligands
different
targets
well
stable
interactions
during
100
ns
MD
simulations.
These
suggest
holds
promise
an
agent
can
combined
antibiotics
treatment
severe
infections.
Nanotechnology Reviews,
Год журнала:
2024,
Номер
13(1)
Опубликована: Янв. 1, 2024
Abstract
Bacterial
biofilms
contribute
to
increased
pathogenesis
and
bacterial
resistance.
Biofilms
can
enhance
pathogenicity
by
shielding
bacteria
from
the
immune
system
antibiotics,
they
are
associated
with
persistent
infections.
Additionally,
antibiotic
resistance
mechanisms
within
make
them
challenging
treat,
emphasizing
need
for
strategies
be
addressed.
Mitigating
virulence
is
a
promising
strategy
that
could
ease
their
eradication
host
immunity
without
stressing
induce
The
merits
of
this
augmented
when
using
safe
anti-virulence
candidates
in
proper
formulations.
current
study
aimed
evaluate
antibiofilm
efficacy
curcumin–zein
nanoparticles
against
Pseudomonas
aeruginosa
.
In
vitro
investigations
were
performed
assess
effect
on
biofilm
formation,
motility,
production
factors,
including
proteases,
hemolysins,
pyocyanin,
comparison
bulk
curcumin.
Furthermore,
expression
genes
encode
quorum
sensing
(QS)
systems
regulate
was
assessed.
An
silico
done
affinity
curcumin
QS
receptors.
an
vivo
protection
assay
inhibitory
our
preparation
diminishing
P.
aeruginosa’s
capacity
pathogenesis.
results
showed
significant
activities
compared
These
attributed
curcumin’s
interfering
its
virulence.
conclusion,
acquires
anti-QS,
anti-virulence,
vastly
enhanced
upon
loading
zein
nanoparticles.
Biomedicines,
Год журнала:
2023,
Номер
11(12), С. 3161 - 3161
Опубликована: Ноя. 28, 2023
The
development
of
bacterial
resistance
is
an
increasing
global
concern
that
requires
discovering
new
antibacterial
agents
and
strategies.
Bacterial
quorum
sensing
(QS)
systems
play
important
roles
in
controlling
virulence,
their
targeting
could
lead
to
diminishing
pathogenesis.
In
this
context,
QS
without
significant
influence
on
growth
assumed
as
a
promising
strategy
overcome
development.
This
study
aimed
at
evaluating
the
anti-QS
anti-virulence
activities
β-adrenoreceptor
antagonist
propranolol
sub-minimal
inhibitory
concentrations
(sub-MIC)
against
two
Gram-negative
models
Pseudomonas
aeruginosa
Serratia
marcescens.
effect
expression
QS-encoding
genes
was
evaluated.
Additionally,
affinity
receptors
virtually
attested.
sub-MIC
biofilm
formation,
motility,
production
virulent
factors
conducted.
outcomes
combination
with
different
antibiotics
were
assessed.
Finally,
vivo
protection
assay
mice
performed
assess
propranolol’s
lessening
current
findings
emphasized
ability
reduce
formation
biofilms,
virulence
factors.
addition,
decreased
capacity
tested
bacteria
induce
pathogenesis
mice.
Furthermore,
significantly
downregulated
showed
receptors.
synergistically
MICs
bacteria.
conclusion,
might
serve
plausible
adjuvant
therapy
for
treatment
serious
infections
after
further
pharmacological
pharmaceutical
studies.
Frontiers in Pharmacology,
Год журнала:
2024,
Номер
15
Опубликована: Март 12, 2024
The
development
of
resistance
to
carbapenems
in
Klebsiella
pneumoniae
due
the
production
metallo-β-lactamases
(MBLs)
is
a
critical
public
health
problem
because
are
last-resort
drugs
used
for
treating
severe
infections
extended-spectrum
β-lactamases
(ESBLs)
producing
K.
.
Restoring
activity
by
inhibition
valuable
approach
combat
carbapenem
resistance.
In
this
study,
two
well-characterized
clinical
multidrug
and
carbapenem-resistant
isolates
were
used.
sub-inhibitory
concentrations
pantoprazole
well-reported
metallo-β-lactamase
inhibitor
captopril
inhibited
hydrolytic
activities
metallo-β-lactamases,
with
having
more
inhibiting
activities.
Both
drugs,
when
combination
meropenem,
exhibited
synergistic
Pantoprazole
could
also
downregulate
expression
genes
bla
NDM
VIM
A
docking
study
revealed
that
bind
chelate
zinc
ions
New
Delhi
Verona
integron-encoded
MBL
(VIM)
enzymes
higher
affinity
than
control
drug
comparable
natural
ligand
indicating
significant
inhibitory
against
metallo-β-lactamases.
conclusion,
can
be
meropenem
as
new
strategy
serious
caused
Frontiers in Cellular and Infection Microbiology,
Год журнала:
2024,
Номер
14
Опубликована: Апрель 4, 2024
Pseudomonas
aeruginosa
belongs
to
the
critical
pathogens
that
represent
a
global
public
health
problem
due
their
high
rate
of
resistance
as
listed
by
WHO.
P.
can
result
in
many
nosocomial
infections
especially
individuals
with
compromised
immune
systems.
Attenuating
virulence
factors
interference
quorum
sensing
(QS)
systems
is
promising
approach
treat
-resistant
infections.
Thymoquinone
natural
compound
isolated
from
Nigella
sativa
(black
seed)
essential
oil.
In
this
study,
minimum
inhibitory
concentration
thymoquinone
was
detected
followed
investigating
antibiofilm
and
antivirulence
activities
subinhibitory
against
PAO1.
The
effect
on
expression
QS
genes
assessed
quantitative
real-time
PCR,
protective
pathogenesis
PAO1
mice
mouse
survival
test.
significantly
inhibited
biofilm,
pyocyanin,
protease
activity,
swarming
motility.
At
molecular
level,
markedly
downregulated
lasI
,
l
asR
rhlI
rhlR
.
Moreover,
could
protect
pathologic
effects
increasing
20%
100%.
conclusion,
agent
be
used
an
adjunct
therapeutic
antibiotics
attenuate
pathogenicity
Frontiers in Pharmacology,
Год журнала:
2024,
Номер
15
Опубликована: Май 21, 2024
Background
P.
aeruginosa
,
a
significant
bacterium,
can
cause
severe
illness
and
resistance
to
antibiotics.
Quorum
sensing
(QS)
systems
regulate
virulence
factors
production.
Targeting
QS
could
reduce
bacteria
pathogenicity
prevent
antibiotic
resistance.
Cruciferous
vegetables
contain
sulforaphane,
known
for
its
anti-inflammatory,
antioxidant,
anticancer,
antimicrobial
properties.
Aim
We
aimed
examine
the
inhibitory
influences
of
at
sub-inhibitory
concentration
(¼
minimum
concentration,
MIC),
on
in
.
Materials
methods
The
sulforaphane’s
anti-virulence
actions
concentrations
were
explored
vitro
vivo
A
sub-MIC
sulforaphane
was
combined
with
anti-pseudomonal
drugs,
results
this
combination
assessed.
virtual
affinity
receptors
studied,
effect
expression
genes
quantified.
Results
Sulforaphane
significantly
decreased
biofilm
formation,
motility,
ability
withstand
oxidative
stress,
synthesis
extracellular
enzymes
such
as
proteases,
hemolysins,
elastase,
well
other
like
pyocyanin.
In
addition,
lessened
severity
infection
mice.
reduced
antipseudomonal
antibiotics’
MICs
when
used
together,
resulting
synergistic
effects.
observed
impacts
attributed
inhibit
via
suppressing
genes’
expression.
Conclusion
shows
promise
potent
anti-QS
agent
that
be
alongside
conventional
antimicrobials
manage
infections
effectively.
Furthermore,
study
paves
way
further
investigation
similar
structures
pharmacophores
candidates.
Microorganisms,
Год журнала:
2023,
Номер
11(10), С. 2420 - 2420
Опубликована: Сен. 28, 2023
The
problem
of
antibiotic
resistance
is
a
global
critical
public
health
concern.
In
light
the
threat
returning
to
pre-antibiotic
era,
new
alternative
approaches
are
required
such
as
quorum-sensing
(QS)
disruption
and
virulence
inhibition,
both
which
apply
no
discernible
selective
pressure
on
bacteria,
therefore
mitigating
potential
for
development
resistant
strains.
Bearing
in
mind
significant
role
QS
orchestrating
bacterial
virulence,
disrupting
becomes
essential
effectively
diminishing
virulence.
This
study
aimed
assess
use
sub-inhibitory
concentration
(0.25
mg/mL)
glyceryl
trinitrate
(GTN)
inhibit
Serratia
marcescens
Pseudomonas
aeruginosa.
GTN
could
decrease
expression
genes
tested
bacteria
manner.
Histopathological
revealed
ability
alleviate
congestion
hepatic
renal
tissues
infected
mice
reduce
leukocyte
infiltration.
recommends
topical
treat
infection
caused
by
P.
aeruginosa
S.
combination
with
antibiotics.
