International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(18), С. 10090 - 10090

Опубликована: Сен. 19, 2024

The emergence of antibiotic-resistant Streptococcus pneumoniae necessitates the discovery novel therapeutic agents. This study investigated antimicrobial potential green-synthesized gold nanoparticles (AuNPs) fabricated using Arthrospira platensis extract. Characterization Fourier transform infrared spectroscopy revealed presence functional groups such as ketones, aldehydes, and carboxylic acids in capping agents, suggesting their role AuNP stabilization. Transmission electron microscopy demonstrated formation rod-shaped AuNPs with a mean diameter 134.8 nm, determined by dynamic light scattering, zeta −27.2 mV, indicating good colloidal stability. synthesized exhibited potent antibacterial activity against S. pneumoniae, minimum inhibitory concentration (MIC) 12 μg/mL, surpassing efficacy control antibiotic, tigecycline. To elucidate underlying mechanisms action, an untargeted metabolomic analysis A. extract was performed, identifying 26 bioactive compounds belonging to diverse chemical classes. In silico studies focused on molecular docking simulations that compound 22 strong binding affinity topoisomerase IV, critical enzyme for bacterial DNA replication. Molecular dynamics further validated stability this protein–ligand complex. These findings collectively highlight promising platensis-derived constituent compounds, warranting investigation development anti-pneumococcal therapeutics.

Язык: Английский