Evaluating the biological (antidiabetic) potential of TEM, FTIR, XRD, and UV‐spectra observed berberis lyceum conjugated silver nanoparticles

Microscopy Research and Technique, Год журнала: 2024, Номер 87(6), С. 1286 - 1305

Опубликована: Фев. 14, 2024

Abstract Diabetes is a life‐threatening disease that affects different parts of the body including liver, kidney, and pancreas. The core root diabetes mainly linked to oxidative stress produced by reactive oxygen species (ROS). Berberis lyceum Royle (BLR) source natural products. It comprises numerous bioactive compounds having antioxidant activities. In current investigation, silver nanoparticles from BLR extract were synthesized, characterized, assessed for antidiabetic potential. UV spectrophotometry, Transmission electron microscopy (TEM), Fourier transform infra‐red spectroscopy (FTIR), x‐ray diffraction (XRD) applied characterization NPs. was evident morphological studies synthesized NPs spherical average size 11.02 nm. Results revealed BLR‐AgNPs showed higher radical scavenging activity as compared extract. Moreover, displayed superior in vivo vitro comparison Glucose level (116.5 ± 5.1 mg/dL), liver function test (ALAT: 54.038 6.2 IU/L; ASAT: 104.42 13.9 ALP: 192.6 2.4 bilirubin: 1.434 0.14 mg/dL; total protein: 5.14 0.24 renal (urea: 39.6 0.63 uric acid: 21.4 0.94 creatinine: 0.798 0.03 albumin: 4.14 0.2 lipid profile (cholesterol: 101.62 3 triglyceride: 110.42 7 HDL‐C: 29.7 LDL‐C: 47.056 1 VLDL‐C: 22.0 1.3 mg/dL) hematology (WBCs: 3.82 10 /μL; RBCs: 4.78 0.42 6 Hb: 12.6 1.0 g/dL; Hematocrit: 39.4 3.7%; MCV: 65.8 fL; platelets: 312 22.4; neutrophils: 34.8 1.87; eosinophils: 3.08 0.43; monocytes: 0.28; lymphocytes: 75.6 3.77) confirmed significant potential BLR‐AgNPs. Histopathological examination authenticated caused revival morphology Hence, findings study suggested potent agent could be an appropriate nanomedicine prevent future. Research Highlights reducing, capping, stabilization synthesis Evaluation α‐amylase inhibition, antioxidant, α‐glucosidase inhibition Thorough using infrared spectroscopy, microscopy, diffraction, UV–VIS spectrophotometer, which st its kind In‐vivo evaluation through multiple biomarkers

Язык: Английский

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Mitochondria Play Essential Roles in Intracellular Protection against Oxidative Stress—Which Molecules among the ROS Generated in the Mitochondria Can Escape the Mitochondria and Contribute to Signal Activation in Cytosol?

Biomolecules, Год журнала: 2024, Номер 14(1), С. 128 - 128

Опубликована: Янв. 19, 2024

Questions about which reactive oxygen species (ROS) or nitrogen (RNS) can escape from the mitochondria and activate signals must be addressed. In this study, two parameters, calculated dipole moment (debye, D) permeability coefficient (Pm) (cm s−1), are listed for hydrogen peroxide (H2O2), hydroxyl radical (•OH), superoxide (O2•−), hydroperoxyl (HO2•), nitric oxide (•NO), dioxide (•NO2), peroxynitrite (ONOO−), peroxynitrous acid (ONOOH) in comparison to those water (H2O). O2•− is generated mitochondrial electron transport chain (ETC), several other ROS RNS subsequently. The candidates pass through membrane include with a small number of dipoles, i.e., H2O2, HO2•, ONOOH, •OH, •NO. results show that •NO2 0.35 D, indicating permeability; however, eliminated quickly. moments •OH (1.67 ONOOH (1.77 indicate they might permeable. This study also suggests play central role protecting against further oxidative stress cells. amounts, long half-life, diffusion distance, Pm, one-electron reduction potential, pKa, rate constants reaction ascorbate glutathione various ROS/RNS, singlet (1O2), O2•−, •NO, •NO2, ONOO−, compared H2O (O2). Molecules negative electrical charges cannot directly diffuse phospholipid bilayer membranes. Short-lived molecules, such as would difficult contribute intracellular signaling. Finally, HO2• were selected ROS/RNS membrane.

Язык: Английский

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Oxidative stress mediates the association between triglyceride-glucose index and risk of cardiovascular and all-cause mortality in metabolic syndrome: evidence from a prospective cohort study

Frontiers in Endocrinology, Год журнала: 2024, Номер 15

Опубликована: Авг. 20, 2024

Background The aim of this study was to investigate the relationship between triglyceride-glucose (TyG) index and cardiovascular disease (CVD) all-cause mortality in adults with metabolic syndrome (MeS) explore mediating role oxidative stress. Methods This included 6131 MeS from National Health Nutrition Examination Survey (NHANES). relationships TyG were elucidated using multivariate Cox proportional hazards models, restricted cubic splines (RCS) Fine-Gray competing risk model. In addition, mediation analysis used test indirect effect stress indicators. Results Over a median 106-month follow-up, total 357 CVD 1292 deaths recorded. After adjustment, there J-type mortality, optimal inflection point 9.13 8.92. threshold point, positively associated (HR: 4.21, 95%CI: 1.82, 9.78) mortality(HR: 2.93, 2.05, 4.18). Even non-cardiovascular as competitive risk, model also illustrated that cumulative incidence higher >9.13 (Fine-Gray P< 0.01). Mediation revealed biomarkers stress, including gamma-glutamyl transferase uric acid, collectively mediated 10.53% association 8.44% (P < 0.05). Conclusion cohort study, found have J-shaped population may play key relationship.

Язык: Английский

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Reactive Oxygen Species: Role in Pathophysiology, and Mechanism of Endogenous and Dietary Antioxidants during Oxidative Stress

Chonnam Medical Journal, Год журнала: 2025, Номер 61(1), С. 32 - 32

Опубликована: Янв. 1, 2025

Redox imbalances, which result from excessive production of reactive oxygen species (ROS) or malfunctioning the antioxidant system, are source oxidative stress. ROS affects all structural and functional components cells, either directly indirectly. In addition to causing genetic abnormalities, also oxidatively modifies proteins by protein oxidation peroxidation alters lipid structure via advanced lipoxidation, decreasing function promoting damage cell death. On other hand, low levels constitute important redox-signaling molecules in various pathways that maintain cellular homeostasis regulate key transcription factors. As a result, can affect processes, such as apoptosis, migration, differentiation, proliferation. act signaling molecules, controlling normal physiological activities at level. Furthermore, there is an increasing body evidence indicating role clinical conditions. this review, we will summarize pathological processes action during

Язык: Английский

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Protective role of sulforaphane in lipid metabolism-related diseases

Molecular Biology Reports, Год журнала: 2025, Номер 52(1)

Опубликована: Фев. 17, 2025

Язык: Английский

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Exploring the Link between Oxidative Stress, Selenium Levels, and Obesity in Youth

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(13), С. 7276 - 7276

Опубликована: Июль 2, 2024

Obesity is a worldwide increasing concern. Although in adults this easily estimated with the body mass index, children, who are constantly growing and whose bodies changing, reference points to assess weight status age gender, need corroboration complementary data, making their quantification highly difficult. The present review explores interaction spectrum of oxidative stress, selenium status, obesity children adolescents. Any factor related stress that triggers and, conversely, induces part vicious circle, complex chain mechanisms derive from each other reinforce serious health consequences. Selenium its compounds exhibit key antioxidant activity also have significant role nutritional evaluation obese children. balance intake, retention, metabolism emerges as vital aspect health, reflecting interactions between diet, obesity. Understanding whether contributor or consequence could inform interventions public strategies aimed at preventing managing an early age.

Язык: Английский

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Insulin resistance in NSCLC: unraveling the link between development, diagnosis, and treatment

Frontiers in Endocrinology, Год журнала: 2024, Номер 15

Опубликована: Фев. 20, 2024

Lung cancer is responsible for the highest number of cancer-related deaths, with non-small cell lung (NSCLC) being most prevalent subtype. A critical aspect managing reducing morbidity and mortality rates among NSCLC patients. Identifying high-risk factors facilitating early diagnosis are invaluable in achieving this objective. Recent research has highlighted association between insulin resistance development NSCLC, further emphasizing its significance context cancer. It been discovered that improving can potentially inhibit progression Consequently, paper aims to delve into occurrence resistance, mechanisms underlying involvement development, as well potential value predicting, assessing, treating

Язык: Английский

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Resveratrol alleviates Mono-2-ethylhexyl phthalate-induced mitophagy, ferroptosis, and immunological dysfunction in grass carp hepatocytes by regulating the Nrf2 pathway

Journal of Environmental Management, Год журнала: 2024, Номер 371, С. 123235 - 123235

Опубликована: Ноя. 6, 2024

Язык: Английский

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Identification of mitophagy-related key genes and their correlation with immune cell infiltration in acute myocardial infarction via bioinformatics analysis

Frontiers in Cardiovascular Medicine, Год журнала: 2025, Номер 11

Опубликована: Янв. 13, 2025

Background Acute myocardial infarction (AMI), a subset of acute coronary syndrome, remains the major cause mortality worldwide. Mitochondrial dysfunction is critically involved in AMI progression, and mitophagy plays vital role eliminating damaged mitochondria. This study aimed to explore mitophagy-related biomarkers their potential molecular basis AMI. Methods datasets (GSE24519 GSE34198) from Gene Expression Omnibus database were combined batch effects removed. Differentially expressed genes (DEGs) selected, intersected with for DEGs (MRDEGs), then subjected enrichment analyses. Next, MRDEGs screened using machine learning methods (logistic regression analysis, RandomForest, least absolute shrinkage selection operator) construct diagnostic risk model select key The efficacy was evaluated nomogram. Moreover, infiltration patterns different immune cells two groups compared. We also explored interactions between themselves or miRNAs/transcription factors (TFs) drug compounds visualized protein structure genes. Finally, we validated expression plasma samples patients an healthy individuals. Results 28 Based on methods, 12 model, including AGPS, CA2, CAT, LTA4H, MYO9B, PRDX6, PYGB, SIRT3, TFEB, TOM1, UBA52 , UBB . nomogram further revealed accuracy diagnosis. found lower abundance such as gamma delta T natural killer high-risk group, showed significant correlation levels both groups. 64 miRNA–mRNA pairs, 75 TF–mRNA 119 RNA-binding protein–mRNA 32 drug–mRNA pairs obtained interaction networks. Conclusions In total, identified constructed findings this might provide novel improving detection

Язык: Английский

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Emerging roles of the acid sphingomyelinase/ceramide pathway in metabolic and cardiovascular diseases: Mechanistic insights and therapeutic implications

World Journal of Cardiology, Год журнала: 2025, Номер 17(2)

Опубликована: Фев. 24, 2025

Metabolic diseases have emerged as a leading cause of mortality from non-communicable diseases, posing significant global public health challenge. Although the association between ceramides (Cers) and metabolic is well-established, role acid sphingomyelinase (ASMase)/Cer pathway in these remains underexplored. This review synthesizes recent research on biological functions, regulatory mechanisms, targeted therapies related to ASMase/Cer conditions, including obesity, diabetes, non-alcoholic fatty liver disease, cardiovascular disease. The effects disease-related indicators, such glycolipid metabolism, insulin resistance, inflammation, mitochondrial homeostasis are elucidated. Moreover, this article discusses therapeutic strategies using inhibitors for inverse prevention treatment light possible efficacy blockade arresting progression diseases. These insights offered herein should provide insight into contribution offer tools develop interventions pathologies their severe complications.

Язык: Английский

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