Metabolic characterization of alkane monooxygenases and the growth phenotypes of Pseudomonas aeruginosa ATCC 33988 on hydrocarbons DOI Creative Commons
Thusitha S. Gunasekera,

Loryn L. Bowen,

Jhoanna C. Alger

и другие.

Journal of Bacteriology, Год журнала: 2025, Номер unknown

Опубликована: Март 11, 2025

ABSTRACT There is a demand and widespread interest in evaluating microbial community structures metabolic processes hydrocarbon environments. The current work aims to detect subgroups (phenotypic subsets) their processes, such as substrate specificity expression of niche-associated genes. In this study, we were able discriminate different cell types real time from complex sample matrix allow the detection live, dead, injured populations jet fuels. We found that alkB1 alkB2 genes induced growth-dependent manner induction started before . This indicates an early response Pseudomonas aeruginosa cells’ exposure alkanes, cells activate gene induction. Deletion completely inhibited P. ATCC 33988 growth fuel, suggesting two alkane monooxygenases are responsible for degradation alkanes fuel. Interestingly, AlkB2 has broader ( n -C8 -n -C16) range compared AlkB1 -C12- -C16). data indicate utilization pathways can coexist 33988, they differentially expressed -C6 -n- C16 study provided additional information on heterogeneity phenotypic diversity within same species after hydrocarbons. advances our understanding provides new insight into metabolism IMPORTANCE Alkane allows natural breakdown hydrocarbons crude oil, which significantly contribute environmental remediation. process microbes not well understood. originally isolated fuel tank, degrades effectively outcompetes type strain PAO1. differential monooxygenase relative importance these degradation. subsets genotype, influenced by stress. Overall, research conducted contributes knowledge about structure

Язык: Английский

Metabolic characterization of alkane monooxygenases and the growth phenotypes of Pseudomonas aeruginosa ATCC 33988 on hydrocarbons DOI Creative Commons
Thusitha S. Gunasekera,

Loryn L. Bowen,

Jhoanna C. Alger

и другие.

Journal of Bacteriology, Год журнала: 2025, Номер unknown

Опубликована: Март 11, 2025

ABSTRACT There is a demand and widespread interest in evaluating microbial community structures metabolic processes hydrocarbon environments. The current work aims to detect subgroups (phenotypic subsets) their processes, such as substrate specificity expression of niche-associated genes. In this study, we were able discriminate different cell types real time from complex sample matrix allow the detection live, dead, injured populations jet fuels. We found that alkB1 alkB2 genes induced growth-dependent manner induction started before . This indicates an early response Pseudomonas aeruginosa cells’ exposure alkanes, cells activate gene induction. Deletion completely inhibited P. ATCC 33988 growth fuel, suggesting two alkane monooxygenases are responsible for degradation alkanes fuel. Interestingly, AlkB2 has broader ( n -C8 -n -C16) range compared AlkB1 -C12- -C16). data indicate utilization pathways can coexist 33988, they differentially expressed -C6 -n- C16 study provided additional information on heterogeneity phenotypic diversity within same species after hydrocarbons. advances our understanding provides new insight into metabolism IMPORTANCE Alkane allows natural breakdown hydrocarbons crude oil, which significantly contribute environmental remediation. process microbes not well understood. originally isolated fuel tank, degrades effectively outcompetes type strain PAO1. differential monooxygenase relative importance these degradation. subsets genotype, influenced by stress. Overall, research conducted contributes knowledge about structure

Язык: Английский

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