L‐Citrulline Alleviates Testicular Ischemia/Reperfusion Injury in Rats by Modulating eNOS/iNOS Induced Nitric Oxide Production, Inflammation, and Apoptosis

Journal of Experimental Zoology Part A Ecological and Integrative Physiology, Год журнала: 2025, Номер unknown

Опубликована: Март 10, 2025

Testicular ischemia/reperfusion injury (TI/RI) is a significant clinical contributor to subfertility and infertility resulting from testicular torsion subsequent detortion. Insufficient nitric oxide (NO) synthesis in TI/RI can result endothelial dysfunction, as the vascular endothelium fails produce sufficient NO sustain appropriate vasodilation blood perfusion. Many studies have found that plays an important role I/RI its increase or decrease affect progression outcome of I/RI. However, controversial complicated. derived by synthase (eNOS) shows protective I/RI, while excessive inducible (iNOS) accelerates inflammation increases oxidative stress, further aggravating Nevertheless, overexpression eNOS may exacerbate Here we try investigate new progress understanding roles during This study examined interplay between cytotoxic cytoprotective mechanisms underpinning produced L-citrulline (L-Cit) on TI/R injured rats. Thirty-two adult Sprague-Dawley albino rats were equally randomized into following groups: normal control group, sham group (3 h/4 h), + L-Cit (600 mg/kg) orally at 1 h before reperfusion. Compared TI/R-operated injection markedly enhanced serum concentrations reproductive hormones (p < 0.05). Elevated SOD, CAT, GPx activity, along with reduced MDA concentrations, indicated diminished stress. The levels TNF-α, IL-1β, caspase-3, BAX, eNOS, iNOS, NF-κB p65 reduced. Histopathological analysis corroborated effect L-Cit. findings confirmed molecular models, demonstrating inhibited IKKβ. results showed giving torsioned made protected them against hormonal imbalance, inflammation, apoptosis makes even more for protecting tissue surgery. not only promoted through activation, but it also facilitated neutralization iNOS production pathogenic reperfusion phase I/R-injured

Язык: Английский

The protective effect of Bromelain on Testicular Dysfunction and Oxidative Stress Induced by REM Sleep Deprivation in Adult Male Rats

Tissue and Cell, Год журнала: 2025, Номер unknown, С. 102927 - 102927

Опубликована: Апрель 1, 2025

Язык: Английский