Expression of Tumor Suppressor FHIT Is Regulated by the LINC00173-SNAIL Axis in Human Lung Adenocarcinoma

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(23), С. 17011 - 17011

Опубликована: Ноя. 30, 2023

Long non-coding RNAs (lncRNAs) play a critical role in variety of human diseases such as cancer. Here, to elucidate novel function lncRNA called LINC00173, we investigated its binding partner, target gene, and regulatory mechanism lung adenocarcinoma, including the A549 cell line patients. In line, RNA immunoprecipitation (RIP) assays revealed that LINC00173 efficiently binds SNAIL. RNA-seq RT-qPCR analyses expression FHIT was decreased upon depletion, indicating is gene LINC00173. Overexpression SNAIL suppressed depletion increased FHIT, negatively regulates FHIT. The downregulation restored by additional revealing LINC00173-SNAIL-FHIT axis for regulation. Data from 501 patients with adenocarcinoma also support existence axis, correlated positively (p = 1.75 × 10-6) 7.00 10-5). Taken together, propose inhibiting adenocarcinoma. Thus, this study sheds light on which may be key carcinogenesis progression

Язык: Английский

CIRCVMA21-RELATED PATHWAY ALLEVIATES LIPOPOLYSACCHARIDE-INDUCED HK-2 CELL INJURY

Shock, Год журнала: 2024, Номер 62(1), С. 119 - 126

Опубликована: Март 25, 2024

ABSTRACT Background : It is reported that circVMA21 has an inhibition effect on sepsis-induced acute kidney injury (AKI). Therefore, the underlying molecular mechanisms of in AKI are worthy further investigation. Material and Methods Lipopolysaccharide (LPS) was used to induce HK2 cell injury. CircVMA21, miR-337-3p ZEB2 expression tested by qRT-PCR. Cell growth detected CCK8 assay, EdU flow cytometry. Protein levels were examined western blot. The inflammatory factors oxidative stress markers measured evaluate response stress. RNA relationship as verified dual-luciferase reporter RIP pull-down assay. Results CircVMA21 had decreased patients. Overexpressed alleviated LPS-induced inflammation, apoptosis, Moreover, sponged miR-337-3p, targeted ZEB2. inhibitory reversed overexpression, overexpression abolished promotion Conclusions could inhibit via miR-337-3p/ZEB2 axis.

Язык: Английский

ZEB1 as a marker of metastasis in thyroid carcinomas: review of literature and own data

INTERNATIONAL JOURNAL OF ENDOCRINOLOGY (Ukraine), Год журнала: 2024, Номер 20(7), С. 517 - 528

Опубликована: Ноя. 28, 2024

Metastases are the leading cause of death from malignant tumors. The metastatic process involves exit cancer cells primary tumor into blood or other transport system, colonization and proliferation in a distant organ. key cascade is epithelial-mesenchymal transition (EMT), which consists transformation epithelial mesenchymal, capable for migration. At same time, markers (E-cadherin) replaced by mesenchymal ones (N-cadherin, vimentin, fibronectin). In tumors, EMT initiated different signaling pathways through regulation transcription factors (EMT-TFs) miRNAs. Downregulation E-cadherin expression induced transforming growth factor β, epidermal factor, fibroblast interleukin-6. Growth activate intracellular pathways, turn induce several repressors gene transcription, such as Snail, Slug, zinc finger E-box binding homeobox 1 (ZEB1), ZEB2, Twist, TBX-2, SIX. ZEB1 main EMT-TF ZEB family associated with cellular plasticity, dissemination, switch dormant to proliferative phenotype at sites determinant poorer clinical prognosis most human cancers. forefront involved control promotes cell migration diffusion, stemness, resistance treatment, metastasizing evasion immune response. Since regulator EMT, stem formation therapy many cancers, including papillary thyroid carcinoma. It may be an important marker invasiveness. Preoperative examination its level plasma carcinomas can help determining extent surgical intervention subsequent radioiodine therapy.

Язык: Английский