Urinary exosomal microRNA-200 family: diagnostic biomarkers for Kawasaki disease and their link to inflammatory markers DOI Creative Commons
Yuzhong Wang, Daqing Sun,

Hui-Zhong Hu

и другие.

Cardiology in the Young, Год журнала: 2024, Номер 35(2), С. 324 - 331

Опубликована: Ноя. 29, 2024

The microRNA-200 family plays a key role in inflammatory and vascular processes, making it relevant target for Kawasaki disease, vasculitis with coronary complications. This study aimed to evaluate the diagnostic potential of urinary exosomal members disease patients. Urine samples from 15 patients healthy controls underwent total exosome isolation high-throughput sequencing. Differential expression was validated using quantitative real-time polymerase chain reaction. Diagnostic assessed via receiver operating characteristic analysis, correlations clinical parameters were evaluated Spearman correlation. High-throughput sequencing identified upregulation microRNA-429, microRNA-200b-3p/5p, microRNA-141-3p, microRNA-200a-3p/5p, microRNA-200c-3p We confirmed significant analysis showing high these microRNAs (area under curves 0.844, 0.791, 0.942, 0.842, 0.898, respectively) combined yielding perfect area curve 1.000. MicroRNA-141 microRNA-200c-3p/5p, however, showed no value. MicroRNA-200a-3p microRNA-200a-5p positively correlated white blood cells, platelet counts, C-reactive protein, while microRNA-200b-3p microRNA-429 erythrocyte sedimentation rate, protein. microRNA-200b-5p moderate counts rate. Urinary members, especially demonstrate strong correlating markers. microRNA-200c did not utility.

Язык: Английский

Connecting the Dots: How MicroRNAs Link Asthma and Atherosclerosis DOI Open Access

Răzvan-Ionuț Zimbru,

Elena-Larisa Zimbru,

Florina Bojin

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(8), С. 3570 - 3570

Опубликована: Апрель 10, 2025

Asthma and atherosclerosis are chronic conditions with distinct pathophysiologies, but overlapping inflammatory mechanisms that suggest a potential common regulatory framework. MicroRNAs (miRNAs), small non-coding RNA molecules modulate gene expression post-transcriptionally, could be key players in linking these disorders. This review outlines how miRNAs contribute to the complex interplay between asthma atherosclerosis, focusing on involved pathways, immune cell regulation vascular remodeling. We discuss specific miRNAs, such as miR-155, miR-21 miR-146a, which have been shown cytokine production T differentiation, impacting respiratory cardiovascular health. The found both emphasize their role biomarkers, also therapeutic targets. Understanding molecular connections may unlock novel approaches for innovative, integrated treatment strategies address significantly improve patient outcomes. Further research is needed explore mechanistic pathways validate translational of miRNA-based interventions preclinical clinical settings.

Язык: Английский

Процитировано

0
Emerging Roles of ncRNAs in Type 2 Diabetes Mellitus: From Mechanisms to Drug Discovery DOI Creative Commons
Yue Yang, Hao Cheng

Biomolecules, Год журнала: 2024, Номер 14(11), С. 1364 - 1364

Опубликована: Окт. 27, 2024

Type 2 diabetes mellitus (T2DM), a high-incidence chronic metabolic disorder, has emerged as global health issue, where most patients need lifelong medication. Gaining insights into molecular mechanisms involved in T2DM development is expected to provide novel strategies for clinical prevention and treatment. Growing evidence validates that non-coding RNAs (ncRNAs) including microRNAs (miRNAs), long (lncRNAs), circular (circRNAs) function crucial regulators multiple biological processes of T2DM, inspiring various potential targets drug candidates. In this review, we summarize the current understanding ncRNA roles discuss use ncRNAs active molecules discovery.

Язык: Английский

Процитировано

1
Extracellular vesicles: immunomodulation, diagnosis, and promising therapeutic roles for rheumatoid arthritis DOI Creative Commons
Desalegn Abebaw, Yibeltal Akelew, Adane Adugna

и другие.

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Ноя. 18, 2024

Extracellular vesicles (EV) can be produced as part of pathology and physiology with increased amounts in pathological conditions. EVs carry transfer cargo such proteins, nucleic acids, lipids to target cells mediate intercellular communication resulting modulation gene expression, signaling pathways, phenotype recipient cells. greatly influence the extracellular environment immune response. Their immunomodulatory properties are crucial rheumatoid arthritis (RA), a condition marked by dysregulated modulate functions innate adaptive RA pathogenesis. Differentially expressed EV-associated molecules RA, microRNAs (miRNAs), long-noncoding RNAs (lncRNAs), messenger (mRNAs) proteins promising markers diagnose disease. miRNA, lncRNA, circular RNA (circRNA) cargos EV regulate inflammation pathogenic fibroblast-like synoviocytes (RA-FLS). Downregulated tissue drugs encapsulated for therapy. This review provides an updated overview EVs’ immunomodulatory, diagnostic, therapeutic roles, particularly emphasizing mesenchymal stem cell-derived (MSC-EVs).

Язык: Английский

Процитировано

0
Urinary exosomal microRNA-200 family: diagnostic biomarkers for Kawasaki disease and their link to inflammatory markers DOI Creative Commons
Yuzhong Wang, Daqing Sun,

Hui-Zhong Hu

и другие.

Cardiology in the Young, Год журнала: 2024, Номер 35(2), С. 324 - 331

Опубликована: Ноя. 29, 2024

The microRNA-200 family plays a key role in inflammatory and vascular processes, making it relevant target for Kawasaki disease, vasculitis with coronary complications. This study aimed to evaluate the diagnostic potential of urinary exosomal members disease patients. Urine samples from 15 patients healthy controls underwent total exosome isolation high-throughput sequencing. Differential expression was validated using quantitative real-time polymerase chain reaction. Diagnostic assessed via receiver operating characteristic analysis, correlations clinical parameters were evaluated Spearman correlation. High-throughput sequencing identified upregulation microRNA-429, microRNA-200b-3p/5p, microRNA-141-3p, microRNA-200a-3p/5p, microRNA-200c-3p We confirmed significant analysis showing high these microRNAs (area under curves 0.844, 0.791, 0.942, 0.842, 0.898, respectively) combined yielding perfect area curve 1.000. MicroRNA-141 microRNA-200c-3p/5p, however, showed no value. MicroRNA-200a-3p microRNA-200a-5p positively correlated white blood cells, platelet counts, C-reactive protein, while microRNA-200b-3p microRNA-429 erythrocyte sedimentation rate, protein. microRNA-200b-5p moderate counts rate. Urinary members, especially demonstrate strong correlating markers. microRNA-200c did not utility.

Язык: Английский

Процитировано

0