Nanomaterial-enabled portable paper-based colorimetric and fluorometric devices: Progress in point-of-care diagnosis

Coordination Chemistry Reviews, Год журнала: 2024, Номер 514, С. 215919 - 215919

Опубликована: Май 12, 2024

Язык: Английский

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Recent advances of nucleic acid-based cancer biomarkers and biosensors

Coordination Chemistry Reviews, Год журнала: 2023, Номер 497, С. 215456 - 215456

Опубликована: Сен. 26, 2023

Язык: Английский

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Amplification-Free Analysis of Bladder Cancer MicroRNAs on Wrinkled Silica Nanoparticles with DNA-Functionalized Quantum Dots

Analytical Chemistry, Год журнала: 2024, Номер 96(12), С. 4860 - 4867

Опубликована: Март 13, 2024

Bladder cancer (BC) occurrence and progression are accompanied by alterations in microRNAs (miRNAs) expression levels. Simultaneous detection of multiple miRNAs contributes to the accuracy reliability BC diagnosis. In this work, wrinkled silica nanoparticles (WSNs) were applied as microreactor for multiplex analysis without enzymes or nucleic acid amplification. Conjugated on surface WSNs, S9.6 antibody was adopted universal module binding DNA/miRNA duplexes, regardless their sequence. Furthermore, single-stranded DNA (ssDNA) labeled with quantum dots (QDs) identifying a given miRNA form QDs-ssDNA/miRNA, which enabled specific capture corresponding QDs WSNs. Based fluorescence signals that ultimately focused target could be sensitively identified femtomolar level (5 fM) wide dynamic range up 6 orders magnitude. The proposed strategy achieved high specificity obviously distinguish single-base mutation sequences possessed assay capability. Moreover, exhibited excellent practicability clinical serum specimens.

Язык: Английский

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Development of a New Route for the Immobilization of Unmodified Single-Stranded DNA on Chitosan Beads and Detection of Released Guanine after Hydrolysis

Molecules, Год журнала: 2023, Номер 28(5), С. 2088 - 2088

Опубликована: Фев. 23, 2023

In this work, chitosan beads were used as a cost-effective platform for the covalent immobilization of unmodified single-stranded DNA, using glutaraldehyde cross-linking agent. The immobilized DNA capture probe was hybridized in presence miRNA-222 complementary sequence. target evaluated based on electrochemical response released guanine, hydrochloride acid hydrolysis Differential pulse voltammetry technique and screen-printed electrodes modified with COOH-functionalized carbon black to monitor guanine before after hybridization. functionalized provided an important signal amplification compared other studied nanomaterials. Under optimal conditions (6 M HCl at 65 °C 90 min), electrochemical-based label-free genosensor assay exhibited linear range between 1 nM µM miRNA-222, detection limit 0.2 miRNA-222. developed sensor successfully quantify human serum sample.

Язык: Английский

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A portable smartphone detection of ctDNA using MnB2 nanozyme and paper-based analytical device

Talanta, Год журнала: 2024, Номер 278, С. 126523 - 126523

Опубликована: Июль 6, 2024

Язык: Английский

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Current trends and advances in nanozyme-enabled miRNA (bio)sensing: Classification, activity and application

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 113127 - 113127

Опубликована: Фев. 1, 2025

Язык: Английский

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Advances in paper and microfluidic based miniaturized systems for cancer biomarkers detection

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 113257 - 113257

Опубликована: Март 1, 2025

Язык: Английский

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Glucose oxidase/copper‑carbon dots/hyaluronic acid self-assembly for self-supply hydrogen peroxide in a double-enzyme cascade to enhance anti-tumor therapy

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 310, С. 143286 - 143286

Опубликована: Апрель 17, 2025

Язык: Английский

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Smartphone-based device for rapid and single-step detection of piRNA–651 using anti-DNA:RNA hybrid antibody and enzymatic signal amplification

Analytica Chimica Acta, Год журнала: 2024, Номер 1305, С. 342583 - 342583

Опубликована: Апрель 11, 2024

Язык: Английский

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A Magnetoelectrochemical Bioassay for Highly Sensitive Sensing of Point Mutations in Interleukin-6 Gene Using TMB as a Hybridization Intercalation Indicator

Biosensors, Год журнала: 2023, Номер 13(2), С. 240 - 240

Опубликована: Фев. 8, 2023

Point mutations are common in the human DNA genome and closely related to higher susceptibility cancer diseases. Therefore, suitable methods for their sensing of general interest. In this work, we report on a magnetic electrochemical bioassay using probes tethered streptavidin beads (strep-MBs) detect T > G single nucleotide polymorphism (SNP) within inteleukin-6 (IL6) gene genomic DNA. presence target fragment tetramethylbenzidine (TMB), signal oxidation TMB is observed, which much than one obtained absence target. The key parameters affecting analytical signal, such as concentration biotinylated probe, its incubation time with strep-MBs, hybridization time, loading, were optimized intensity signal-to-blank (S/B) ratio selection criteria. Using spiked buffer solutions, can mutated allele wide range concentrations (over six decades) low detection limit (7.3 fM). Furthermore, displays high specificity major (one mismatched), two mismatched non-complementary More importantly, variation scarcely diluted DNA, collected from 23 donors, reliably distinguish between heterozygous (TG genotype) homozygous (GG respect control subjects (TT genotype), where differences statistically highly significant (p-value < 0.001). Thus, useful cohort studies targeting or more

Язык: Английский

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