Biosensors,
Год журнала:
2025,
Номер
15(3), С. 189 - 189
Опубликована: Март 15, 2025
This
review
explores
the
development,
technological
foundations,
and
applications
of
biosensor
technologies
across
various
fields,
such
as
medicine
for
disease
diagnosis
monitoring,
food
industry.
However,
primary
focus
is
on
their
use
in
detecting
contaminants
pathogens,
well
environmental
monitoring
water
quality
assessment.
The
classifies
different
types
biosensors
based
bioreceptor
transducer,
highlighting
how
they
are
specifically
designed
detection
emerging
(ECs)
pathogens
water.
Key
innovations
this
technology
critically
examined,
including
advanced
techniques
systematic
evolution
ligands
by
exponential
enrichment
(SELEX),
molecularly
imprinted
polymers
(MIPs),
self-assembled
monolayers
(SAMs),
which
enable
fabrication
sensors
with
improved
sensitivity
selectivity.
Additionally,
integration
microfluidic
systems
into
analyzed,
demonstrating
significant
enhancements
performance
speed.
Through
these
advancements,
work
emphasizes
fundamental
role
key
tools
safeguarding
public
health
preserving
integrity.
Chemosensors,
Год журнала:
2025,
Номер
13(1), С. 12 - 12
Опубликована: Янв. 9, 2025
The
increasing
incidence
of
organophosphate
(OP)
pesticide
poisoning
and
the
use
OP
chemical
warfare
agents
(CWA)
in
conflicts
terrorist
acts
need
sustainable
methods
for
sensing,
decontamination,
detoxification
compounds.
Enzymes
can
serve
as
specific,
cost-effective
biosensors
OPs.
We
will
report
on
recent
advancements
carboxylesterases
from
Hormone-Sensitive
Lipase
detection
In
addition,
enzymatic-based
decontamination
offer
long-term,
environmentally
friendly
benefits
compared
to
conventional
such
treatment,
incineration,
neutralization,
volatilization.
Enzymatic
has
gained
attention
an
alternative
traditional
OP-detoxification
methods.
This
review
provides
overview
latest
research
enzymatic
sensing
OPs,
by
exploiting
enzymes,
isolated
thermophilic/extremophilic
Bacteria
Archaea
that
show
exceptional
thermal
stability
other
harsh
conditions.
Finally,
we
make
examples
integration
between
systems,
including
protein
engineering
enhance
OP-degrading
activities
detailed
characterization
best
variants.
Micro and Nano Systems Letters,
Год журнала:
2025,
Номер
13(1)
Опубликована: Май 12, 2025
Abstract
Nanoparticles
(NPs)
are
redefining
enzyme
immobilization,
offering
a
paradigm
shift
in
biocatalysis
through
precision
engineering
at
the
nanoscale.
With
their
exceptional
surface
area,
tunable
porosity,
and
customizable
functionalities,
NPs
provide
unprecedented
control
over
stability,
activity,
adaptability,
bridging
gap
between
molecular-scale
interactions
industrial-scale
applications.
In
era
of
intelligent
bioprocessing,
how
can
NP-based
immobilization
strategies
be
optimized
to
drive
next
frontier
sustainable
high-performance
technologies?
A
deep
understanding
NP
structural
diversity,
interfacial
chemistry,
enzyme-matrix
is
crucial
unlocking
full
potential.
This
review
systematically
explores
emerging
platforms,
including
cross-linked
aggregates
(CLEAs),
covalent
organic
frameworks
(COFs),
nanoflowers,
nanofibers,
carbon
nanotubes
(CNTs),
graphene
oxide
(GO),
ionic
liquids
(ILs),
layered
double
hydroxides
(LDHs),
each
tailored
advantages
for
catalytic
enhancement
process
efficiency.
The
outlines
current
advancements
such
as
3D
printing
wearable
biosensors,
forecasts
integration
artificial
intelligence
smart
nano-biocatalysts,
envisions
futuristic
applications
bio-intelligent
nano/micro-robotic
systems
space
biosensors.
Challenges,
upscaling
limitations,
nanotoxicity
concerns,
environmental
risks,
addressed
ensure
safe
viable
implementation.
provides
structured
roadmap
on
(I)
advances
using
next-generation
NPs,
(II)
challenges
scalability
safety,
(III)
sustainability
benefits
enzyme-based
industrial
biocatalysis,
(IV)
emergence
intelligence,
nanoscale
technologies
AI-assisted
design
optimization.
These
visionary
approaches
mark
toward
dynamic,
adaptive,
highly
specialized,
multifunctional
nano-enzyme
systems.
Graphical
Microorganisms,
Год журнала:
2025,
Номер
13(5), С. 1166 - 1166
Опубликована: Май 21, 2025
Urban
sewage,
aquaculture
wastewater,
and
medical
wastewater
are
significant
reservoirs
transmission
sources
of
Salmonella.
Rapid
detection
Salmonella
is
crucial
for
effectively
reducing
the
risk
disease
safeguarding
public
health.
Differentiating
viable
from
inactivated
cells
presents
challenges,
affecting
accurate
assessment
pathogen
risks.
Moreover,
current
methods
face
several
limitations,
including
lengthy
periods,
high
costs,
limited
applicability,
underscoring
need
rapid,
sensitive,
visual
diagnostic
approaches.
In
this
study,
we
combined
propidium
monoazide
(PMA)
with
recombinase
polymerase
amplification
(RPA)
clustered
regularly
spaced
short
palindromic
repeats
(CRISPR)/Cas12a
systems
to
develop
a
rapid
system
targeting
fimY
gene.
DNA
was
amplified
visually
detected
within
60
min
dead
were
excluded.
We
assessed
specificity
sensitivity
PMA-RPA-CRISPR/Cas12a
assay.
The
results
showed
that
assay
had
level
specificity,
no
reactions
observed
other
pathogens.
application
PMA
has
effect
on
RPA-CRISPR/Cas12a
technology
visibility
fluorescence
reporting
system.
successfully
in
minimum
limit
101
CFU/mL.
summary,
developed
study
allows
at
concentrations
as
low
By
integrating
technology,
offers
valuable
technical
support
efficient,
clear
wastewater.
Critical Reviews in Analytical Chemistry,
Год журнала:
2025,
Номер
unknown, С. 1 - 15
Опубликована: Июнь 1, 2025
Early
detection
of
biological
threat
agents
plays
a
crucial
role
in
public
health
protection,
food
safety,
and
environmental
monitoring.
Bioimmunosensors,
utilizing
specific
antigen-antibody
interactions,
represent
promising
technology
these
applications
due
to
their
high
sensitivity
specificity.
This
review
explores
the
current
state
knowledge
regarding
bioimmunosensors,
highlighting
recent
technological
innovations,
such
as
integration
with
nanotechnology,
microfluidic
techniques,
development
novel
platforms
based
on
optical
electrochemical
biosensors.
Key
challenges
development,
including
stability
elements,
cost
reduction,
scalability
for
real-world
applications,
are
also
discussed.
The
analysis
future
application
prospects
points
growing
potential
bioimmunosensors
areas
medical
diagnostics,
pathogen
environment,
quality
control,
bioterrorism
defense.
Increased
Internet
Things
(IoT)
systems
portable
analytical
devices
may
revolutionize
approach
monitoring
rapid
response
threats
coming
years.
Biophysical Reviews,
Год журнала:
2024,
Номер
17(1), С. 103 - 125
Опубликована: Ноя. 5, 2024
This
article
discusses
the
use
of
diatom
in
biosensing
and
various
applications.
A
thorough
understanding
properties
diatoms
is
essential
for
advancement
life
sciences
technologies.
review
elucidates
emerging
significance
applications
by
highlighting
high
surface
area-to-volume
ratio,
biocompatibility,
facile
functionalization
them.
We
examined
possible
application
as
flexible
systems
detection
analytes,
such
biological
molecules,
heavy
metals,
poisons,
considering
findings
earlier
research.
Additionally,
to
show
how
can
be
used
create
precise
sensitive
biosensors,
integrating
with
several
transduction
modalities,
including
optical,
electrochemical,
piezoelectric
methods.
also
delve
into
potential
diatom-based
future
well
pertinent
topics
repeatability,
stability,
scalability.
provides
an
in-depth
analysis
quickly
evolving
field
biosensing,
which
could
affect
industries,
environmental
monitoring,
food
security
medical
diagnostics.
