Sensitive Detection of Biomarker in Gingival Crevicular Fluid Based on Enhanced Electrochemiluminescence by Nanochannel-Confined Co3O4 Nanocatalyst

Biosensors, Год журнала: 2025, Номер 15(1), С. 63 - 63

Опубликована: Янв. 19, 2025

The sensitive detection of inflammatory biomarkers in gingival crevicular fluid (GCF) is highly desirable for the evaluation periodontal disease. Luminol-based electrochemiluminescence (ECL) immunosensors offer a promising approach fast and convenient biomarkers. However, luminol’s low ECL efficiency under neutral conditions remains challenge. This study developed an immunosensor by engineering immunorecognition interface on outer surface mesoporous silica nanochannel film (SNF) confining Co3O4 nanocatalyst within SNF nanochannels to improve luminol efficiency. was grown indium tin oxide (ITO) electrode using simple Stöber solution growth method. A successfully confined through situ electrodeposition, confirmed X-ray photoelectron spectroscopy (XPS) electrochemical measurements. demonstrated excellent electrocatalytic activity, effectively enhancing H2O2 oxidation boosting signal conditions. Using interleukin-6 (IL-6) as proof-of-concept demonstration, epoxy functionalization enabled covalent immobilization capture antibodies, forming specific interface. IL-6 binding induced immunocomplex formation, which reduced allowed quantitative detection. showed linear range from 1 fg mL−1 10 ng mL−1, with limit (LOD) 0.64 mL−1. It also good selectivity anti-interference capabilities, enabling successful artificial GCF samples.

Язык: Английский

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Enhanced electrochemiluminescence of luminol at neutral medium using nanochannel-confined Co3O4 nanozyme for highly sensitive detection of tumor biomarker

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 112903 - 112903

Опубликована: Янв. 1, 2025

Язык: Английский

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Carbon Nitride Nanosheets as an Adhesive Layer for Stable Growth of Vertically-Ordered Mesoporous Silica Film on a Glassy Carbon Electrode and Their Application for CA15-3 Immunosensor

Molecules, Год журнала: 2024, Номер 29(18), С. 4334 - 4334

Опубликована: Сен. 12, 2024

Vertically ordered mesoporous silica films (VMSF) are a class of porous materials composed ultrasmall pores and ultrathin perpendicular nanochannels, which attractive in the areas electroanalytical sensors molecular separation. However, VMSF easily falls off from carbonaceous electrodes thereby impacts their broad applications. Herein, carbon nitride nanosheets (CNNS) were served as an adhesive layer for stable growth on glassy electrode (GCE). CNNS bearing plentiful oxygen-containing groups can covalently bind with silanol VMSF, effectively promoting stability GCE surface. Benefiting numerous open nanopores modification VMSF's external surface carbohydrate antigen 15-3 (CA15-3)-specific antibody allows target-controlled transport electrochemical probes through internal yielding sensitive quantitative detection CA15-3 range 1 mU/mL to 1000 U/mL low limit 0.47 mU/mL. Furthermore, proposed VMSF/CNNS/GCE immunosensor is capable highly selective accurate determination spiked serum samples, offers simple effective strategy various practical biomarkers complicated biological specimens.

Язык: Английский

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Homogeneous Aptasensor with Electrochemical and Electrochemiluminescence Dual Detection Channels Enabled by Nanochannel-Based Probe Enrichment and DNase I Cleavage for Tumor Biomarker Detection

Molecules, Год журнала: 2025, Номер 30(3), С. 746 - 746

Опубликована: Фев. 6, 2025

Homogeneous aptasensors that eliminate the need for probe labeling or immobilization hold significant potential rapid detection of tumor biomarkers. Herein, a homogeneous aptasensor with electrochemical (EC) and electrochemiluminescence (ECL) dual channels was developed by integrating nanochannel-based enrichment DNase I cleavage selective biomarker, carbohydrate antigen 125 (CA125). A two-dimensional (2D) composite prepared assembling CA125-specific aptamer cationic tris(2,2′-bipyridyl)Ru(II) (Ru(bpy)32+), which exhibited both EC ECL properties, onto graphene oxide (GO) nanosheets (Ru(bpy)32+/Apt@GO). vertically ordered mesoporous silica film (VMSF) ultrasmall, uniform, aligned nanochannel arrays rapidly grown on inexpensive disposable indium tin (ITO) electrode, forming interface. Due to size exclusion effect ultrasmall nanochannels in VMSF, Ru(bpy)32+/Apt@GO unable penetrate nanochannels, resulting no detectable Ru(bpy)32+ signal electrode. Upon specific recognition CA125 aptamer, an aptamer-CA125 complex formed subsequently detached from GO. then cleaved complex, releasing allowing dissociate into solution. This enzymatic enabled re-enter binding cycle, amplifying release The electrostatic adsorption VMSF significantly enhanced signals. constructed linear range 0.1 U/mL 100 ng/mL, limit (LOD) 91 mU/mL. For detection, detected over 0.001 U/mL, LOD as low 0.4 demonstrated excellent selectivity successfully applied dual-mode EC/ECL fetal bovine serum samples.

Язык: Английский

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Nanochannel-Confined Ni(OH)2-CeO2 Composite Nanozyme Boosts Electrochemiluminescence of Luminol-Dissolved Oxygen for Immunosensing

Biosensors and Bioelectronics, Год журнала: 2025, Номер unknown, С. 117451 - 117451

Опубликована: Апрель 1, 2025

Язык: Английский

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A Novel Aggregation-Induced Emission-Based Electrochemiluminescence Aptamer Sensor Utilizing Red-Emissive Sulfur Quantum Dots for Rapid and Sensitive Malathion Detection

Biosensors, Год журнала: 2025, Номер 15(1), С. 64 - 64

Опубликована: Янв. 20, 2025

Rapid, effective, and cost-effective methods for large-scale screening of pesticide residues in the environment agricultural products are important assessing potential environmental risks safeguarding human health. Here, we constructed a novel aggregation-induced emission (AIE) electrochemical aptamer (Apt) sensor based on red-emissive sulfur quantum dots (SQDs), which aimed at rapid quantitative detection malathion. SQDs were prepared using two-step oxidation method with good electrochemiluminescence (ECL) optical properties. These modified onto electrode surface to serve as ECL luminophores. Subsequently, Apt was introduced form double-helix structure complementary chain (cDNA). The signal reduced because biomolecules had poor electrical conductivity inefficient electron transfer. When target malathion added, double helix unraveled, fell off surface, restored. linear range 1.0 × 10−13–1.0 10−8 mol·L−1, limit 0.219 fM. successful preparation not only develops properties but also expands application sensing.

Язык: Английский

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Schiff Base-Crosslinked Tetra-PEG-BSA Hydrogel: Design, Properties, and Multifunctional Functions

Journal of Functional Biomaterials, Год журнала: 2025, Номер 16(2), С. 69 - 69

Опубликована: Фев. 18, 2025

Hydrogel network structures play a crucial role in determining mechanical properties and have broad applications biomedical industrial fields. Therefore, their rational design is essential. Herein, we developed Schiff base-crosslinked hydrogel through the reaction of Tetra-armed polyethylene glycol with aldehyde end groups (Tetra-PEG-CHO) bovine serum albumin (BSA) under alkaline conditions. In addition, Tetra-PEG-BSA showed rapid gelation time around 11 s, much faster than that GLU-BSA, HT-BSA, GDL-BSA hydrogels. It had high optical transmittance (92.92% at 600 nm) swelling ratios superior to other gels different solutions, maintaining structural integrity even denaturing environments such as guanidine hydrochloride SDS. Mechanical tests strain break (84.12 ± 0.76%), rupture stress (28.64 1.21 kPa), energy dissipation ability (468.0 34.9 kJ·m-3), surpassing all control group MTT cytotoxicity assays indicated cell viability remained >80% lower concentrations, confirming excellent biocompatibility. These findings suggest hydrogels may serve effective materials for drug delivery, tissue engineering, 3D printing.

Язык: Английский

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Highly sensitive electrochemical immunosensor based on methylene blue-reduced graphene oxide nanocomposites as signal probes for IL-6 detection in gingival crevicular fluid samples

Frontiers in Chemistry, Год журнала: 2025, Номер 13

Опубликована: Апрель 2, 2025

As an important inflammatory cytokine, interleukin-6 (IL-6) can mediate the entire pathological process of periodontitis and is closely associated with degree inflammation. Therefore, it critical to develop convenient quantitative methods for monitoring IL-6 quantity in gingival crevicular fluid. In this study, methylene blue (MB)-decorated reduced graphene oxide (rGO) employed as signal probe further support antibody-enabling specific recognition IL-6. Due π-π stacking electrostatic interactions, rGO-MB nanocomposites be stably obtained. rGO good conductivity large surface area characteristics promotes redox signals MB on glassy carbon electrode (GCE). addition, through simple situ self-polymerization dopamine, polydopamine (PDA) obtained not only directly used a biological crosslinking agent covalent immobilization anti-IL-6 antibody but also regarded protective layer enhance stability GCE surface. Such designed PDA/rGO-MB/GCE-based immunosensor enables binding produces decreased electrochemical MB, realizing selective sensitive measurement Consequently, our fabricated has excellent linear relationship ranging from 1 pg/mL 100 ng/mL, limit detection low 0.48 pg/mL. Moreover, as-prepared sensing strategy shows accurate fluid samples.

Язык: Английский

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A dual-functional antibiofouling and signal amplification sensing platform enabling accurate analysis in complicated biological samples

Sensors and Actuators B Chemical, Год журнала: 2025, Номер unknown, С. 137856 - 137856

Опубликована: Апрель 1, 2025

Язык: Английский

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Graphitic carbon nitride nanosheet supported silica nanochannel film for enhanced electrochemiluminescence sensing of 2,4,6-trichlorophenol and prochloraz

RSC Advances, Год журнала: 2024, Номер 14(39), С. 28976 - 28983

Опубликована: Янв. 1, 2024

Graphitic carbon nitride nanosheet supported silica nanochannel film is easily fabricated for enhanced electrochemiluminescence sensing of 2,4,6-trichlorophenol and prochloraz.

Язык: Английский

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