Mapping of Functional Metabolic Phenotypes in Acute Myeloid Leukemia DOI Creative Commons
Sissel E. Dyrstad, Kimberley Joanne Hatfield,

Endre Stigen

и другие.

Cancer Medicine, Год журнала: 2025, Номер 14(10)

Опубликована: Май 1, 2025

ABSTRACT Background Acute myeloid leukemia (AML) is an aggressive hematologic malignancy with a poor prognosis, particularly in older patients. AML highly heterogeneous, influenced by various chromosomal, genetic, and epigenetic alterations. Methods This study investigated the metabolic profiles of primary cells from 46 patients, focusing on mitochondrial respiration glycolysis. We hypothesized that would reflect distinct disease characteristics. Using Seahorse technology, we measured oxygen consumption rate (OCR) for extracellular acidification (ECAR) Results Our results showed significant variability activity, some samples relying more glycolysis than respiration. Mature (FAB M4/M5, CD34 negative) exhibited increased rates both glycolysis, indicating adaptations. Higher glycolytic activity was observed patients adverse cytogenetic abnormalities. However, no clear associations were found between mutations FLT3 or NPM1 . Conclusion These findings highlight role suggest targeting specific pathways could offer therapeutic opportunities, subgroups like FAB M4/M5 unique features. Further studies are needed to refine these strategies clinical application.

Язык: Английский

Immunity and Coagulation in COVID-19 DOI Open Access
П. П. Авдонин,

Maria S. Blinova,

Anastasia A. Serkova

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(20), С. 11267 - 11267

Опубликована: Окт. 19, 2024

Discovered in late 2019, the SARS-CoV-2 coronavirus has caused largest pandemic of 21st century, claiming more than seven million lives. In most cases, COVID-19 disease by virus is relatively mild and affects only upper respiratory tract; it often manifests itself with fever, chills, cough, sore throat, but also less-common symptoms. patients do not require hospitalization, fully recover. However, some infection leads to development a severe form COVID-19, which characterized life-threatening complications affecting lungs, other organs systems. particular, various forms thrombotic are common among COVID-19. The mechanisms for remain unclear. Accumulated data indicate that pathogenesis based on disruptions functioning innate immune key role primary response viral assigned two These pattern recognition receptors, primarily members toll-like receptor (TLR) family, complement system. Both systems first engage fight against launch whole range aimed at its rapid elimination. Normally, their joint activity destruction pathogen recovery. these can cause an excessive inflammatory dangerous body. turn, inflammation entails activation damage vascular endothelium, as well hypercoagulable state observed seriously ill Activation endothelium hypercoagulation lead thrombosis and, result, tissues. Immune-mediated termed "immunothrombosis". this review, we discuss detail features immunothrombosis associated potential underlying mechanisms.

Язык: Английский

Процитировано

3

Mapping of Functional Metabolic Phenotypes in Acute Myeloid Leukemia DOI Creative Commons
Sissel E. Dyrstad, Kimberley Joanne Hatfield,

Endre Stigen

и другие.

Cancer Medicine, Год журнала: 2025, Номер 14(10)

Опубликована: Май 1, 2025

ABSTRACT Background Acute myeloid leukemia (AML) is an aggressive hematologic malignancy with a poor prognosis, particularly in older patients. AML highly heterogeneous, influenced by various chromosomal, genetic, and epigenetic alterations. Methods This study investigated the metabolic profiles of primary cells from 46 patients, focusing on mitochondrial respiration glycolysis. We hypothesized that would reflect distinct disease characteristics. Using Seahorse technology, we measured oxygen consumption rate (OCR) for extracellular acidification (ECAR) Results Our results showed significant variability activity, some samples relying more glycolysis than respiration. Mature (FAB M4/M5, CD34 negative) exhibited increased rates both glycolysis, indicating adaptations. Higher glycolytic activity was observed patients adverse cytogenetic abnormalities. However, no clear associations were found between mutations FLT3 or NPM1 . Conclusion These findings highlight role suggest targeting specific pathways could offer therapeutic opportunities, subgroups like FAB M4/M5 unique features. Further studies are needed to refine these strategies clinical application.

Язык: Английский

Процитировано

0