The Size and Localization of Ribeye and GluR2 in the Auditory Inner Hair Cell Synapse of C57BL/6 Mice Are Affected by Short-Pulse Corticosterone in a Sex-Dependent Manner DOI Creative Commons
Ewa Domarecka, Heidi Olze, Agnieszka J. Szczepek

и другие.

Brain Sciences, Год журнала: 2025, Номер 15(5), С. 441 - 441

Опубликована: Апрель 24, 2025

Background: Inner hair cell (IHC) ribbon synapses are the initial in auditory pathway, comprising presynaptic ribbons and postsynaptic glutamate receptors on peripheral afferent fibers. The excitatory neurotransmitter primarily signals through AMPA-type heterotetrameric (AMPARs), composed of GluR1, GluR2, GluR3, GluR4 subunits. Research shows that corticosterone affects AMPA receptor subunits central nervous system. present study investigates effects murine cochlea. Methods: Cochlear explants were isolated from male female C57BL/6 pups (postnatal days 4-5), treated for 20 min with 100 nM corticosterone, cultured an additional 24 h. concentration AMPAR protein was quantified using ELISA assay, while gene expression analyzed RT-PCR. synaptic localization patterns GluR2 Ribeye examined immunofluorescence confocal microscopy. Results: Male mice have a significantly greater basal subunit than females more puncta per IHC females. Corticosterone increases size males twofold GluR2/Ribeye colocalization apical region Conclusions: Exposure membranous cochleae to induces changes consistent neuroplasticity periphery. observed effect is sex-dependent.

Язык: Английский

The Size and Localization of Ribeye and GluR2 in the Auditory Inner Hair Cell Synapse of C57BL/6 Mice Are Affected by Short-Pulse Corticosterone in a Sex-Dependent Manner DOI Creative Commons
Ewa Domarecka, Heidi Olze, Agnieszka J. Szczepek

и другие.

Brain Sciences, Год журнала: 2025, Номер 15(5), С. 441 - 441

Опубликована: Апрель 24, 2025

Background: Inner hair cell (IHC) ribbon synapses are the initial in auditory pathway, comprising presynaptic ribbons and postsynaptic glutamate receptors on peripheral afferent fibers. The excitatory neurotransmitter primarily signals through AMPA-type heterotetrameric (AMPARs), composed of GluR1, GluR2, GluR3, GluR4 subunits. Research shows that corticosterone affects AMPA receptor subunits central nervous system. present study investigates effects murine cochlea. Methods: Cochlear explants were isolated from male female C57BL/6 pups (postnatal days 4-5), treated for 20 min with 100 nM corticosterone, cultured an additional 24 h. concentration AMPAR protein was quantified using ELISA assay, while gene expression analyzed RT-PCR. synaptic localization patterns GluR2 Ribeye examined immunofluorescence confocal microscopy. Results: Male mice have a significantly greater basal subunit than females more puncta per IHC females. Corticosterone increases size males twofold GluR2/Ribeye colocalization apical region Conclusions: Exposure membranous cochleae to induces changes consistent neuroplasticity periphery. observed effect is sex-dependent.

Язык: Английский

Процитировано

0