The role of cuproptosis-related gene in the classification and prognosis of melanoma

Frontiers in Immunology, Год журнала: 2022, Номер 13

Опубликована: Окт. 19, 2022

Background Melanoma, as one of the most aggressive and malignant cancers, ranks first in lethality rate skin cancers. Cuproptosis has been shown to paly a role tumorigenesis, However, cuproptosis melanoma metastasis are not clear. Studying correlation beteen molecular subtypes cuproptosis-related genes (CRGs) may provide some guidance for prognosis melanoma. Methods We collected 1085 samples The Cancer Genome Atlas(TCGA) Gene Expression Omnibus(GEO) databases, constructed CRGs gene according clinical characteristics, investigated metastasis. randomly divide into train set validation ratio 1:1. A prognostic model was using data from then validated on set. performed tumor microenvironment analysis drug sensitivity analyses high low risk groups based outcome score. Finally, we established metastatic Results According expression levels 12 genes, obtained three 1 , B C . Among them, subtype had best survival outcome. Based differentially expressed shared by genotypes, results 2 group Then, consisting 6 key variable which could more accurately predict 1-, 3-, 5-year overall rates patients. Besides, 98 drugs were screened out. explored seven genes. Conclusions In conclusion, play melanoma, also new insights underlying pathogenesis

Язык: Английский

NOVA1 acts as an oncogenic RNA-binding protein to regulate cholesterol homeostasis in human glioblastoma cells

Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(10)

Опубликована: Фев. 28, 2024

NOVA1 is a neuronal RNA-binding protein identified as the target antigen of rare autoimmune disorder associated with cancer and neurological symptoms, termed paraneoplastic opsoclonus-myoclonus ataxia. Despite strong association between cancer, it has been unclear how function might contribute to biology. In this study, we find that acts an oncogenic factor in GBM (glioblastoma multiforme) cell line established from patient. Interestingly, Argonaute (AGO) CLIP common 3′ untranslated region (UTR) targets, which were down-regulated knockdown cells, indicating transcriptome-wide intersection AGO–microRNA (miRNA) targets regulation. binding 3′UTR stabilized transcripts including those encoding cholesterol homeostasis related proteins. Selective inhibition NOVA1–RNA interactions antisense oligonucleotides disrupted fitness. The precision our studies point both mechanism precise RNA sequence sites selectively inhibit interactions. Taken together, commonly overexpressed GBM, where can antagonize AGO2–miRNA actions consequently up-regulates synthesis, promoting viability.

Язык: Английский

A comprehensive survey on the use of deep learning techniques in glioblastoma

Artificial Intelligence in Medicine, Год журнала: 2024, Номер 154, С. 102902 - 102902

Опубликована: Июнь 5, 2024

Язык: Английский