Advances in Biological Regulation, Год журнала: 2022, Номер 87, С. 100936 - 100936
Опубликована: Ноя. 29, 2022
Язык: Английский
Cell Proliferation, Год журнала: 2024, Номер unknown
Опубликована: Ноя. 14, 2024
Abstract The phosphatidylinositol 3‐kinase/Protein Kinase B/mammalian target of rapamycin (PI3K/AKT/mTOR) signalling pathway is pivotal in various cancers, including T‐cell acute lymphoblastic leukaemia (T‐ALL), a particularly aggressive type leukaemia. This study investigates the effects Neosetophomone B (NSP‐B), meroterpenoid fungal metabolite, on T‐ALL cell lines, focusing its anti‐cancer mechanisms and therapeutic potential. NSP‐B significantly inhibited proliferation cells by inducing G0/G1 cycle arrest promoting caspase‐dependent apoptosis. Additionally, led to dephosphorylation subsequent inactivation PI3K/AKT/mTOR pathway, critical survival growth. Molecular docking studies revealed strong binding affinity active site AKT, primarily involving key residues crucial for activity. Interestingly, treatment also induced apoptosis reduced phytohemagglutinin‐activated primary human CD3 + T cells, accompanied arrest. Importantly, did not affect normal indicating degree selectivity action, targeting only activated cells. In conclusion, our findings highlight potential as novel agent T‐ALL, specifically aberrantly being selective action. These results provide basis further investigation into NSP‐B's properties application clinical therapies.
Язык: Английский
Процитировано
0
Molecular Diversity, Год журнала: 2024, Номер unknown
Опубликована: Дек. 18, 2024
Язык: Английский
Процитировано
0
Molecules, Год журнала: 2023, Номер 28(4), С. 1687 - 1687
Опубликована: Фев. 10, 2023
Human papilloma virus (HPV) causes cervical and many other cancers. Recent trend in vaccine design is shifted toward epitope-based developments that are more specific, safe, easy to produce. In this study, we predicted eight immunogenic peptides of CD4+ CD8+ T-lymphocytes (MHC class I II as M1 M2) including early proteins (E2 E6), major (L1) minor capsid protein (L2). Male female Sprague Dawly rats groups were immunized with each synthetic peptide. L1M1, L1M2, L2M1, L2M2 induced significant response compared E2M1, E2M2, E6M1 E6M2. We observed optimal titer IgG antibodies (>1.25 g/L), interferon-γ (>64 ng/L), granzyme-B (>40 pg/mL) control at second booster dose (240 µg/500 µL). The induction peptide-specific indicates the T-cell dependent B-lymphocyte activation. A substantial cell count was 240 µL. male rats, for L1 L2 peptide 3000 3118, 3369 3484 respectively control. conclusion, demonstrated likely contain potential epitopes immune responses supporting feasibility peptide-based development HPV.
Язык: Английский
Процитировано
1
Pharmaceuticals, Год журнала: 2023, Номер 16(11), С. 1515 - 1515
Опубликована: Окт. 24, 2023
Ovarian cancer (OC) is one of the most lethal gynecological malignancies. The use biological compounds such as non-coding RNAs (ncRNAs) being considered a therapeutic option to improve or complement current treatments since deregulation ncRNAs has been implicated in pathogenesis and progression OC. Old drugs with antitumoral properties have also studied context cancer, although their antitumor mechanisms are not fully clear. For instance, antidiabetic drug metformin shown pleiotropic effects several vitro models including Interestingly, reported regulate ncRNAs, which could explain its diverse on tumor cells. In this review, we discuss mechanism epigenetic regulation described for metformin, focus evidence metformin-dependent microRNA (miRNAs) long RNA (lncRNAs)
Язык: Английский
Процитировано
0
Advances in Biological Regulation, Год журнала: 2022, Номер 87, С. 100936 - 100936
Опубликована: Ноя. 29, 2022
Язык: Английский
Процитировано
0