Integrating Single-Cell and Spatial Transcriptomics To Uncover and Elucidate a GP73-Mediated Pro-Angiogenic Regulatory Networks in Hepatocellular Carcinoma DOI
Jiazhou Ye, Xing Gao,

Xi Huang

и другие.

Опубликована: Янв. 1, 2023

Hepatocellular carcinoma (HCC) was distinctively characterized by the hypervascular nature. Here, we generated a single cell-spatial transcriptomics landscape of HCC vasculogenic ecology and illustrated over-expressed GP73 cells exerting cellular communications with vascular endothelial high pro-angiogenesis potential via multiple receptor-ligand interactions in processing tumor vasculture development. Specifically, uncovered an interactive GP73-mediated regulatory network coordinated c-Myc, lactate, JAK2/STAT3 endoplasmic reticulum stress (ERS) signals cells, elucidated its pro-angiogenic roles vitro vivo. Mechanistically, as pivotal hub gene activated histone lactylation stimulated phosphorylation downstream STAT3 through directly binding simultaneously enhancing enhanced GRP78-induced ERS. Besides, turns potentiated functions. Clinically, serum levels positively correlated response to anti-angiogenic regimens, essential prognostic nomogram showing good predicative performance determining 6-month 1-year survivals for patients treated anti-angiogenesis therapy. Altogether, our data mechanism networks proved crucial angiogenesis niche favorable HCC.Funding: This research granted Guangxi Key Research Development Plan (NO. GUIKEAB19245002); National Natural Science Foundation China (NO.82060427, 82103297); Medical health key discipline construction project; cultivation Scholarship Fund Education Department; Nanning Qingxiu District Technology Project (No. 2020037, 2020038, 2021007, 2021010, 2021012); Advanced Innovation Teams Xinghu Scholars Program University; University Outstanding Young Talents Training Program.Declaration Interest: The authors declare no competing interests.Ethical Approval: All included consented sequencing experimental protocol carried out all procedures approval these provided ethics review committee Cancer Hospital (LW2022040).

Язык: Английский

Integrating Single-Cell and Spatial Transcriptomics To Uncover and Elucidate a GP73-Mediated Pro-Angiogenic Regulatory Networks in Hepatocellular Carcinoma DOI
Jiazhou Ye, Xing Gao,

Xi Huang

и другие.

Опубликована: Янв. 1, 2023

Hepatocellular carcinoma (HCC) was distinctively characterized by the hypervascular nature. Here, we generated a single cell-spatial transcriptomics landscape of HCC vasculogenic ecology and illustrated over-expressed GP73 cells exerting cellular communications with vascular endothelial high pro-angiogenesis potential via multiple receptor-ligand interactions in processing tumor vasculture development. Specifically, uncovered an interactive GP73-mediated regulatory network coordinated c-Myc, lactate, JAK2/STAT3 endoplasmic reticulum stress (ERS) signals cells, elucidated its pro-angiogenic roles vitro vivo. Mechanistically, as pivotal hub gene activated histone lactylation stimulated phosphorylation downstream STAT3 through directly binding simultaneously enhancing enhanced GRP78-induced ERS. Besides, turns potentiated functions. Clinically, serum levels positively correlated response to anti-angiogenic regimens, essential prognostic nomogram showing good predicative performance determining 6-month 1-year survivals for patients treated anti-angiogenesis therapy. Altogether, our data mechanism networks proved crucial angiogenesis niche favorable HCC.Funding: This research granted Guangxi Key Research Development Plan (NO. GUIKEAB19245002); National Natural Science Foundation China (NO.82060427, 82103297); Medical health key discipline construction project; cultivation Scholarship Fund Education Department; Nanning Qingxiu District Technology Project (No. 2020037, 2020038, 2021007, 2021010, 2021012); Advanced Innovation Teams Xinghu Scholars Program University; University Outstanding Young Talents Training Program.Declaration Interest: The authors declare no competing interests.Ethical Approval: All included consented sequencing experimental protocol carried out all procedures approval these provided ethics review committee Cancer Hospital (LW2022040).

Язык: Английский

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