Biochemical Pharmacology, Год журнала: 2023, Номер 218, С. 115864 - 115864
Опубликована: Окт. 18, 2023
Язык: Английский
The AAPS Journal, Год журнала: 2024, Номер 26(5)
Опубликована: Авг. 6, 2024
Язык: Английский
Процитировано
6
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(4), С. 2070 - 2070
Опубликована: Фев. 8, 2024
Chemotherapy is still the mainstay of treatment for triple-negative breast cancer (TNBC) patients. Yet only 20% TNBC patients show a pathologic complete response (pCR) after neoadjuvant chemotherapy. 5-Fluorouracil (5-FU) stable cornerstone in all recommended chemotherapeutic protocols However, patients’ innate or acquired chemoresistance rate 5-FU steeply escalating. This study aims to unravel mechanism behind aggressive cell line, MDA-MB-231 cells, explore further role tumor suppressor microRNAs (miRNAs), miR-1275, miR-615-5p, and Let-7i, relieving TNBC, finally provide translational therapeutic approach co-deliver respective miRNA oligonucleotides using chitosan-based nanoparticles (CsNPs). In this regard, cellular viability proliferation were investigated MTT BrdU assays, respectively. was found induce JAK/STAT PI3K/Akt/mTOR pathways cells with contaminant repression their upstream regulators Let-7i. Moreover, CsNPs prepared ionic gelation method chosen studied as nanovectors combination targeting TNBC. The average particle sizes, surface charges, morphologies different characterized dynamic light scattering (DLS) transmission electron microscopy (TEM), addition, encapsulation efficiency (EE%), drug loading capacity (DLC%), release manner at two pH values assessed. conclusion, novel co-loaded three demonstrated synergistic activity remarkable through alleviating 5-FU.
Язык: Английский
Процитировано
5
Advances in protein chemistry and structural biology, Год журнала: 2025, Номер unknown, С. 97 - 115
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
International Journal of Nanomedicine, Год журнала: 2025, Номер Volume 20, С. 1425 - 1442
Опубликована: Фев. 1, 2025
Abstract: Breast cancer is one of the most common types in women worldwide and a leading cause deaths among women. As result, various treatments have been developed to combat this disease. treatment varies based on its stage type pathology. Among therapeutic options, ultrasound has employed assist breast cancer, including radiation therapy, chemotherapy, targeted immunotherapy, hormonal and, more recently, radiofrequency ablation for early-stage inoperable patients. One notable advancement ultrasound-targeted microbubble destruction (UTMD), which gradually becoming highly effective non-invasive anti-tumor modality. This technique can enhance chemical, genetic, immune, anti-vascular therapies through physical biological effects. Specifically, UTMD improves drug transfer efficiency destroys tumor neovascularization while reducing toxic side effects body during treatment. Given these developments, application ultrasound-assisted therapy gained significant attention from research scholars. In review, we will discuss development modalities importantly, highlight microbubble-targeted disruption techniques Keywords: destruction, microbubble, TME,
Язык: Английский
Процитировано
0
International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(5), С. 2303 - 2303
Опубликована: Март 5, 2025
The metabolic enzyme aldehyde dehydrogenase 1A1 (ALDH1A1), a cancer stem cell marker associated with poor outcomes in breast cancer, has emerged as promising therapeutic target TNBC. aim of this study was to investigate the role ALDH1A1 radiation resistance and redox stress triple negative (TNBC). Functional knockouts were generated by CRISPR/Cas9-mediated deletion SUM159 line, three distinct clonal populations isolated. Genetic targeting confirmed Sanger sequencing, loss protein expression validated Western blotting. assays assessed ALDEFLUOR activity, viability, self-renewal capacity, reactive oxygen species (ROS) levels or without both bulk population lines. Interestingly, activity uniformly lost across all lines; however, functional effects on stress, survival, sensitivity observed only one population. These findings highlight significant variability among populations, reflecting complexity tumor heterogeneity. This underscores importance accounting for heterogeneity when ALDH1A1, certain TNBC subpopulations may rely more heavily function. insights are critical developing effective ALDH1A1-targeted therapies.
Язык: Английский
Процитировано
0
Biochemical and Biophysical Research Communications, Год журнала: 2025, Номер 765, С. 151854 - 151854
Опубликована: Апрель 18, 2025
Язык: Английский
Процитировано
0
Chemico-Biological Interactions, Год журнала: 2025, Номер 415, С. 111530 - 111530
Опубликована: Апрель 26, 2025
Язык: Английский
Процитировано
0
International Journal of Pharmaceutics, Год журнала: 2025, Номер unknown, С. 125655 - 125655
Опубликована: Май 1, 2025
Язык: Английский
Процитировано
0
Biomedicines, Год журнала: 2024, Номер 12(2), С. 272 - 272
Опубликована: Янв. 25, 2024
A group of 27 patients diagnosed with metastatic triple-negative breast cancer (mTNBC) was randomly distributed into two groups and underwent different lines metronomic treatment (mCHT). The former (N 14) received first-line mCHT showed a higher overall survival rate than the second 13), which second-line mCHT. Analysis one patient still alive from first group, mTNBC in 2019, complete metabolic response (CMR) after composite approach implicating followed by epirubicin third-line nab-paclitaxel, chosen for subsequent molecular characterization. We found altered expression stemness-associated gene NOTCH-1 its corresponding protein. Additionally, we changes oncogenes, such as MYC AKT, along their respective proteins. Overall, our data suggest that MTD might be effective negatively regulating stemness traits usually associated emergence drug resistance.
Язык: Английский
Процитировано
2
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(3), С. 1471 - 1471
Опубликована: Янв. 25, 2024
The triple-negative breast cancer (TNBC) subtype is characterized by the lack of expression ERα (estrogen receptor α), PR (progesterone receptor) and no overexpression HER-2. However, TNBC can express androgen (AR) or estrogen β (ERβ). Also, secretes steroid hormones influenced hormonal fluctuations, so inhibition could exert a beneficial effect in treatment. aim this study was to evaluate dutasteride, anastrozole ASP9521 vitro processes using human cell lines. For this, immunofluorescence, sensitivity, proliferation wound healing assays were performed, hormone concentrations studied. Results revealed that all lines expressed AR ERβ; ones them most intensely more sensitive antihormonal treatments. All treatments reduced viability, highlighting MDA-MB-453 SUM-159. Indeed, decrease levels observed these lines, which relate reduction viability. In addition, MCF-7 SUM-159 increased migration under treatments, increasing levels, favor migration. Thus, be for therapies. This clarifies importance ERβ-positive TNBC.
Язык: Английский
Процитировано
2