The role of Prolyl 3-Hydroxylase 1 (P3H1) in tumor development and prognosis: a pan-cancer analysis with validation in colonic adenocarcinoma
American Journal of Translational Research,
Год журнала:
2025,
Номер
17(2), С. 770 - 790
Опубликована: Янв. 1, 2025
Cancer
is
a
multifaceted
disease
characterized
by
unregulated
cell
proliferation,
evasion
of
apoptosis,
and
metastasis.
Recent
studies
have
highlighted
the
importance
extracellular
matrix
remodeling
post-translational
modifications
in
tumorigenesis.
Prolyl
3-hydroxylase
1
(P3H1),
an
enzyme
involved
collagen
hydroxylation,
has
gained
attention
for
its
role
cancer
progression.
This
study
investigates
P3H1
expression,
prognostic
value,
functional
relevance
across
multiple
human
cancers
using
combination
bioinformatic
experimental
approaches.
Using
The
Genome
Atlas
(TCGA)
data
from
TIMER2.0
UALCAN
databases,
we
observed
significant
upregulation
mRNA
protein
various
cancers.
Prognostic
analysis
GEPIA2
KM
plotter
revealed
that
high
expression
correlates
with
poorer
overall
survival
colon
adenocarcinoma
(COAD),
kidney
renal
clear
carcinoma
(KIRC),
liver
hepatocellular
(LIHC).
Further,
genetic
promoter
methylation
analyses
showed
low
mutation
frequencies
reduced
specific
types.
Functional
pathway
enrichment
indicated
formation,
endoplasmic
reticulum
activity,
pathways
such
as
ECM-receptor
interaction
PI3K-Akt
signaling.
Validation
linked
immunosorbent
assay
COAD
patient
serum
samples
demonstrated
significantly
elevated
levels
compared
to
healthy
controls,
AUC
approaching
1.0
receiver
operating
characteristic
(ROC)
curve
analysis.
suggests
potential
diagnostic
biomarker.
Additionally,
experiments
were
conducted
cells
assess
P3H1's
Knockdown
HCT116
resulted
reduction
colony
migratory
abilities
these
cells.
These
findings
emphasize
COAD,
KIRC,
LIHC
pathogenesis
possible
utility
clinical
diagnosis.
Язык: Английский
STX1A regulates ferroptosis and chemoresistance in gastric cancer through mitochondrial function modulation
Human Cell,
Год журнала:
2025,
Номер
38(3)
Опубликована: Март 8, 2025
Язык: Английский
Epigenetic Regulation of DNA Methylation and RNA Interference in Gastric Cancer: A 2024 Update
Biomedicines,
Год журнала:
2024,
Номер
12(9), С. 2001 - 2001
Опубликована: Сен. 3, 2024
Gastric
cancer
(GC)
remains
a
significant
public
health
concern
because
of
its
lethality,
underscoring
the
need
for
deeper
insights
into
molecular
mechanisms.
Recent
studies
have
increasingly
highlighted
role
epigenetic
modifications
as
critical
players
in
progression.
Despite
their
importance,
research
specifically
addressing
factors
GC
is
relatively
scarce.
This
paper
seeks
to
bridge
that
gap
by
examining
recent
literature
elucidates
landscape
associated
with
GC.
The
investigation
long
noncoding
RNAs
(lncRNAs)
has
revealed
substantial
involvement
gene
dysregulation
and
alterations
within
tumors.
Notably,
lncRNAs
such
LINC00853
LINC01266
been
identified
contributors
modulation
expression.
Furthermore,
overexpression
Язык: Английский
Identification and validation of matrix metalloproteinase hub genes as potential biomarkers for Skin Cutaneous Melanoma
Frontiers in Oncology,
Год журнала:
2024,
Номер
14
Опубликована: Окт. 18, 2024
Objectives
The
role
of
matrix
metalloproteinases
(MMPs)
in
Skin
Cutaneous
Melanoma
(SKCM)
development
and
progression
is
unclear
so
far.
This
comprehensive
study
delved
into
the
intricate
MMPs
SKCM
progression.
Methods
RT-qPCR,
bisulfite
sequencing,
WES
analyzed
MMP
gene
expression,
promoter
methylation,
mutations
cell
lines.
TCGA
datasets
validated
findings.
DrugBank
molecular
docking
identified
potential
regulatory
drugs,
line
experiments
confirmed
key
genes
tumorigenesis.
Results
Our
findings
unveiled
significant
up-regulation
MMP9,
MMP12,
MMP14,
MMP16,
coupled
with
hypomethylation
their
promoters
lines,
implicating
involvement
disease
Mutational
analysis
highlighted
a
low
frequency
these
genes,
indicating
less
expression
mechanisms.
Prognostic
assessments
showcased
correlation
between
elevated
poor
overall
survival
(OS)
patients.
Additionally,
functional
involving
silencing
revealed
impact
on
cellular
proliferation,
further
emphasizing
significance
MMP16
pathobiology.
Conclusion
identifies
Estradiol
Calcitriol
as
drugs
for
modulating
SKCM,
highlighting
diagnostic
prognostic
biomarkers.
Язык: Английский
A New Medical Evaluation for Gastric Cancer Patients to Increase the Success Rate of Immunotherapy: A 2024 Update
Pharmaceuticals,
Год журнала:
2024,
Номер
17(9), С. 1121 - 1121
Опубликована: Авг. 24, 2024
Researchers
have
performed
numerous
studies
on
immunotherapy
because
of
the
high
death
rate
associated
with
gastric
cancer
(GC).
GC
research
has
made
tremendous
progress,
and
we
wanted
to
provide
an
update
this
topic.
On
basis
update,
suggest
performing
a
new
medical
evaluation
before
initiating
in
patients
increase
success
immunotherapies.
We
propose
that
start
immunotherapy,
they
should
be
evaluated
given
score
one
two
points
for
following
factors:
immunopathological
features,
molecular
genomic
potential
consequences
bacterial
pathogens,
immunotherapeutic
resistance
hyperprogressive
illness,
use
biomarkers
gauge
their
prognosis
responses
optimize
surgery.
The
proposed
scoring
system
could
also
help
diagnosis
GC.
With
all
advances
genetics,
immunology,
microbiology,
improved,
not
changed.
Currently,
diagnosed
undergo
surgical
resection
as
only
permanent
solution.
Patients
who
meet
maximum
from
presented
proposal
eligible
immediately
after
immunotherapy.
Therefore,
first-line
option
clinicians.
Язык: Английский