International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 2997 - 2997
Опубликована: Март 25, 2025
Liver fibrosis is a pathological manifestation of chronic liver disease developing to the terminal stage, and there lack effective therapeutic drugs in clinical practice. Scutellarin (SCU) flavonoid extracted from Erigeron breviscapus (Vaniot.) Hand.-Mazz., which has significant anti-liver-fibrosis efficacy, but its mode action remains incompletely understood. A model was built with male Sprague Dawley rats induced by CCl4 evaluate effect drugs. 16S rRNA sequencing metabolomics were used analyze regulatory effects SCU on intestinal flora host metabolism; antibiotics administered eliminate gut microbiota fecal transplantation (FMT) experiments verify mechanism. The mechanistic basis underlying SCU's hepatic anti-fibrotic screened network pharmacology combined transcriptomics, molecular docking, qPCR, WB verification. results showed that may play an role correcting imbalance regulating linoleic acid purine metabolic pathways. In addition, can downregulate levels proteins genes related PI3K/AKT axis. summary, alleviates reversing imbalance, profile, inhibiting
Язык: Английский