Current Opinion in Biotechnology,
Год журнала:
2022,
Номер
76, С. 102750 - 102750
Опубликована: Июль 13, 2022
In
recent
years
it
has
been
increasingly
appreciated
that
blood
vessels
are
not
simply
suppliers
of
nutrients
and
oxygen,
but
actually
play
an
exquisite
regulatory
role
in
bone
development
repair.
A
specialized
kind
endothelium,
named
type
H
because
its
high
expression
CD31
Endomucin,
constitutes
anatomically
defined
proximity
the
epiphyseal
growth
plate.
Type
endothelium
regulates
proliferation
differentiation
both
osteoblasts
osteoclasts
through
secretion
angiocrine
signals
is
a
hub
for
bidirectional
molecular
crosstalk
between
different
cell
populations
osteogenic
microenvironment.
key
target
current
translational
approaches
aiming
at
coupling
angiogenesis
osteogenesis
Open
questions
remain
about
their
presence
features
notstereotyped
tissues,
like
engineered
grafts,
opportunities
clinical
stimulation
by
pharmacological
treatments.
Materials Today Bio,
Год журнала:
2023,
Номер
19, С. 100569 - 100569
Опубликована: Фев. 1, 2023
The
key
to
critical
bone
regeneration
in
tissue
engineering
relies
on
an
ideal
bio-scaffold
coated
with
a
controlled
release
of
growth
factors.
Gelatin
methacrylate
(GelMA)
and
Hyaluronic
acid
(HAMA)
have
been
novel
topic
interest
while
introducing
appropriate
nano-hydroxyapatite
(nHAP)
improve
its
mechanical
properties.
And
the
exosomes
derived
from
human
urine-derived
stem
cells
(human
USCEXOs)
also
reported
promote
osteogenesis
engineering.
present
study
aimed
design
new
GelMA-HAMA/nHAP
composite
hydrogel
as
drug
delivery
system.
USCEXOs
were
encapsulated
slow-released
for
better
osteogenesis.
characterization
GelMA-based
showed
excellent
performance
vitro
studies
that
USCEXOs/GelMA-HAMA/nHAP
could
marrow
mesenchymal
(BMSCs)
angiogenesis
endothelial
progenitor
(EPCs),
respectively.
Meanwhile,
vivo
results
confirmed
this
significantly
defect
repair
cranial
rat
model.
In
addition,
we
found
can
formation
H-type
vessels
area,
enhancing
therapeutic
effect.
conclusion,
our
findings
suggested
controllable
biocompatible
may
effectively
by
coupling
angiogenesis.
The
critical
factor
determining
the
in
vivo
effect
of
bone
repair
materials
is
microenvironment,
which
greatly
depends
on
their
abilities
to
promote
vascularization
and
formation.
However,
implant
are
far
from
ideal
candidates
for
guiding
regeneration
due
deficient
angiogenic
osteogenic
microenvironments.
Herein,
a
double-network
composite
hydrogel
combining
vascular
endothelial
growth
(VEGF)-mimetic
peptide
with
hydroxyapatite
(HA)
precursor
was
developed
build
an
microenvironment
repair.
prepared
by
mixing
acrylated
β-cyclodextrins
octacalcium
phosphate
(OCP),
HA
precursor,
gelatin
solution,
followed
ultraviolet
photo-crosslinking.
To
improve
potential
hydrogel,
QK,
VEGF-mimicking
peptide,
loaded
β-cyclodextrins.
QK-loaded
promoted
tube
formation
human
umbilical
vein
cells
upregulated
expression
angiogenesis-related
genes,
such
as
Flt1,
Kdr,
VEGF,
marrow
mesenchymal
stem
cells.
Moreover,
QK
could
recruit
Furthermore,
OCP
be
transformed
into
release
calcium
ions
facilitating
regeneration.
integrated
showed
obvious
osteoinductive
activity.
results
animal
experiments
that
enhanced
skull
defects
rats,
perfect
synergistic
effects
vascularized
In
summary,
improving
microenvironments
our
shows
promising
prospects
Biomacromolecules,
Год журнала:
2023,
Номер
25(4), С. 2075 - 2113
Опубликована: Июль 5, 2023
The
field
of
bone
tissue
engineering
has
seen
significant
advancements
in
recent
years.
Each
year,
over
two
million
transplants
are
performed
globally,
and
conventional
treatments,
such
as
grafts
metallic
implants,
have
their
limitations.
Tissue
offers
a
new
level
treatment,
allowing
for
the
creation
living
within
biomaterial
framework.
Recent
advances
biomaterials
provided
innovative
approaches
to
rebuilding
function
after
damage.
Among
them,
gelatin
methacryloyl
(GelMA)
hydrogel
is
emerging
promising
supporting
cell
proliferation
regeneration,
GelMA
exhibited
exceptional
physicochemical
biological
properties,
making
it
viable
option
clinical
translation.
Various
methods
classes
additives
been
used
application
with
incorporation
nanofillers
or
other
polymers
enhancing
its
resilience
functional
performance.
Despite
results,
fabrication
complex
structures
that
mimic
architecture
provision
balanced
physical
properties
both
vasculature
growth
proper
stiffness
load
bearing
remain
challenges.
In
terms
utilizing
osteogenic
additives,
priority
should
be
on
versatile
components
promote
angiogenesis
osteogenesis
while
reinforcing
structure
applications.
This
review
focuses
efforts
advantages
GelMA-based
composite
engineering,
covering
literature
from
last
five
Advanced Materials,
Год журнала:
2023,
Номер
35(52)
Опубликована: Апрель 23, 2023
Advances
in
bioprinting
have
enabled
the
fabrication
of
complex
tissue
constructs
with
high
speed
and
resolution.
However,
there
remains
significant
structural
biological
complexity
within
tissues
that
is
unable
to
recapitulate.
Bone,
for
example,
has
a
hierarchical
organization
ranging
from
molecular
whole
organ
level.
Current
techniques
materials
employed
imposed
limits
on
scale,
speed,
resolution
can
be
achieved,
rendering
technique
reproduce
hierarchies
cell-matrix
interactions
are
observed
bone.
The
shift
toward
biomimetic
approaches
bone
engineering,
where
hydrogels
provide
biophysical
biochemical
cues
encapsulated
cells,
promising
approach
enhancing
function
development
vitro
modeling.
A
major
focus
modeling
creating
dynamic
microenvironmental
niches
support,
stimulate,
direct
cellular
processes
formation
remodeling.
Hydrogels
ideal
imitating
extracellular
matrix
since
they
engineered
present
various
whilst
allowing
bioprinting.
Here,
recent
advances
3D
niche
conducive
engineering
models
reviewed.
ABSTRACT
The
repair
and
functional
reconstruction
of
bone
defects
resulting
from
trauma,
surgical
resection,
degenerative
diseases,
congenital
malformations
are
major
clinical
challenges.
Bone
tissue
engineering
has
significant
advantages
in
the
treatment
severe
defects.
Vascularized
scaffolds
gradually
attracting
attention
development
because
their
excellent
biomimetic
properties
efficient
efficiency.
Three‐dimensional
(3D)
printing
technology,
which
can
be
used
to
fabricate
structures
at
different
scales
using
a
wide
range
materials,
been
production
vascularized
scaffolds.
This
review
discusses
research
progress
3D
for
Angiogenesis‐osteogenesis
coupling
regeneration
process
is
first
introduced,
followed
by
summary
technologies,
inks,
bioactive
factors
Notably,
this
focuses
on
structural
design
strategies
Finally,
application
medicine,
as
well
challenges
outlooks
future
development,
described.
Antioxidants,
Год журнала:
2022,
Номер
11(2), С. 318 - 318
Опубликована: Фев. 6, 2022
Bone
tissue
engineering
is
a
complex
domain
that
requires
further
investigation
and
benefits
from
data
obtained
over
past
decades.
The
models
are
increasing
in
complexity
as
they
reveal
new
co-culturing
microfluidics
applications.
vitro
now
focus
on
the
3D
medium
of
osteoblasts,
osteoclasts,
osteocytes
utilizing
collagen
for
separation;
this
type
research
allows
controlled
in-depth
analysis.
Oxidative
stress
takes
toll
domain,
being
beneficial
well
destructive.
Reactive
oxygen
species
(ROS)
molecules
influence
differentiation
but
time
their
presence
can
affect
patients
aid
appearance
diseases
such
osteoporosis.
be
limited
by
using
antioxidants
vitamin
K
N-acetyl
cysteine
(NAC).
Scaffolds
biocompatible
coatings
hydroxyapatite
bioactive
glass
required
to
isolate
implant,
protect
zone
metallic,
ionic
exchange,
enhance
bone
regeneration
mimicking
composition
structure
body,
thus
enhancing
cell
proliferation.
materials
functionalized
with
growth
factors
create
better
response
higher
chances
success
clinical
use.
This
review
highlights
vast
majority
newly
information
regarding
engineering,
models,
implant
coatings,
scaffolds,
biomolecules,
techniques
utilized
obtain
them.
