Analysis of four orchid protein-protein interactions networks to elucidate the mechanism of floral morphology diversity formation DOI Creative Commons
Yu Chen, Yiqin Wang,

SUN Bo

и другие.

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Май 30, 2023

Abstract The diversity of Orchidaceae plants and their complex adaptations to pollinators have made a hot topic in the study plant evolution function. However, our current understanding molecular mechanisms behind diverse floral morphologies remains limited.To address this gap knowledge, presents an integration protein network predictions comparative analysis four different orchid species (Apostasia odorata, Phalaenopsis equestris, Erycina pusilla, Dendrobium officinale ) with widely varying organ structures. Our research findings suggest that formation labellum is linked presence or absence AP3-3 SEP4 genes, specific interaction patterns involving these genes each may be key regulating drive differences morphology.Our indicates number type interacting proteins symmetry regulator DIVARICATA play role morphological radial bilateral orchids. Furthermore, both MADS-box MYB are involved pollen development. Additionally, we found MADS-box, MYB, GRAS families regulate flower morphology by influencing hormone signaling pathways during development.To further explore findings, utilized CRISPR/Cas9 technique knock out SYD gene, agrobacterium-mediated genetic transformation Arabidopsis thaliana was used examine effects. results showed increase petal opening mating degree, along changes overlapping stamen distribution.

Язык: Английский

bZIP Transcription Factors: Structure, Modification, Abiotic Stress Responses and Application in Plant Improvement DOI Creative Commons
Zhonglong Guo, Raphael Dzinyela, Liming Yang

и другие.

Plants, Год журнала: 2024, Номер 13(15), С. 2058 - 2058

Опубликована: Июль 25, 2024

Plant growth, yield, and distribution are significantly impacted by abiotic stresses, affecting global ecosystems forestry practices. However, plants have evolved complex adaptation mechanisms governed numerous genes transcription factors (TFs) to manage these stresses. Among these, bZIP (basic leucine zipper) is a crucial regulator orchestrating morphological adaptations. This review aims elucidate the multifaceted roles of TFs in plant species. We discuss changes induced stress stimuli pivotal functions mediating responses. While several publications explored response this delves into intricate regulatory networks, summarizing alternative splicing post-translational modifications, signaling networks interacting with TFs, genetic engineering TFs. By synthesizing current research, provides an updated discussion on interactions other proteins regulate stresses such as cold, heat, drought, salt. Additionally, it offers avenues for future research applications improve resilience through engineering.

Язык: Английский

Процитировано

16
Recent advances in understanding of the epigenetic regulation of plant regeneration DOI Creative Commons
Xuemei Liu,

Kehui Zhu,

Jun Xiao

и другие.

aBIOTECH, Год журнала: 2023, Номер 4(1), С. 31 - 46

Опубликована: Янв. 16, 2023

Ever since the concept of "plant cell totipotency" was first proposed in early twentieth century, plant regeneration has been a major focus study. Regeneration-mediated organogenesis and genetic transformation are important topics both basic research modern agriculture. Recent studies model

Язык: Английский

Процитировано

21
Towards a hierarchical gene regulatory network underlying somatic embryogenesis DOI

Fuxiang Wang,

Guan-Dong Shang, Jiawei Wang

и другие.

Trends in Plant Science, Год журнала: 2022, Номер 27(12), С. 1209 - 1217

Опубликована: Июль 6, 2022

Язык: Английский

Процитировано

22
The roles of epigenetic regulators in plant regeneration: Exploring patterns amidst complex conditions DOI Creative Commons
Jiawen Li, Qiyan Zhang, Zejia Wang

и другие.

PLANT PHYSIOLOGY, Год журнала: 2024, Номер 194(4), С. 2022 - 2038

Опубликована: Янв. 30, 2024

Abstract Plants possess remarkable capability to regenerate upon tissue damage or optimal environmental stimuli. This ability not only serves as a crucial strategy for immobile plants survive through harsh environments, but also made numerous modern plant improvements techniques possible. At the cellular level, this biological process involves dynamic changes in gene expression that redirect cell fate transitions. It is increasingly recognized chromatin epigenetic modifications, both activating and repressive, intricately interact regulate process. Moreover, outcomes of regulation on regeneration are influenced by factors such differences regenerative species donor types, well concentration timing hormone treatments. In review, we focus several well-characterized modifications their regulatory roles widely studied morphogenic regulators, aiming enhance our understanding mechanisms which govern regeneration.

Язык: Английский

Процитировано

5
Transcriptomic profiling reveals histone acetylation-regulated genes involved in somatic embryogenesis in Arabidopsis thaliana DOI Creative Commons
Barbara Wójcikowska, Karolina Chwiałkowska, Katarzyna Nowak

и другие.

BMC Genomics, Год журнала: 2024, Номер 25(1)

Опубликована: Авг. 15, 2024

Abstract Background Somatic embryogenesis (SE) exemplifies the unique developmental plasticity of plant cells. The regulatory processes, including epigenetic modifications controlling embryogenic reprogramming cell transcriptome, have just started to be revealed. Results To identify genes histone acetylation-regulated expression in SE, we analyzed global transcriptomes Arabidopsis explants undergoing induction response treatment with deacetylase inhibitor, trichostatin A (TSA). TSA-induced and auxin (2,4-dichlorophenoxyacetic acid; 2,4-D)-induced were compared. RNA-seq results revealed similarities TSA- auxin-induced transcriptomic responses that involve extensive deregulation, mostly repression, majority genes. Within differentially expressed (DEGs), identified master regulators (transcription factors - TFs) involved biosynthesis, signaling, polar transport NITRILASE-encoding function indole-3-acetic acid (IAA) biosynthesis. TSA-upregulated TF essential functions included LEC1/LEC2 , FUS3 AGL15 MYB118 PHB PHV PLTs WUS/WOXs . transcriptome also upregulation stress-related genes, those related stress hormone In line data, accumulated salicylic (SA) abscisic (ABA), suggesting role acetylation (Hac) regulating hormone-related during SE induction. Since adaxial side cotyledon explant contributes induction, organ polarity-related responding TSA treatment, AIL7/PLT7 RGE1 LBD18 40 HB32 CBF1 ULT2 Analysis relevant mutants supported Conclusion study provide a step forward deciphering network transition somatic cells plants.

Язык: Английский

Процитировано

4
Epigenetic Regulation of Androgenic Responses in Camelina sativa L. Through Trichostatin A DOI

Zahra Behzadfar,

Mohammad Reza Abdollahi

Journal of Plant Growth Regulation, Год журнала: 2025, Номер unknown

Опубликована: Фев. 2, 2025

Язык: Английский

Процитировано

0
Functional Genomics of Legumes in Bulgaria—Advances and Future Perspectives DOI Open Access
Miglena Revalska,

Mariana Radkova,

Miroslava Zhiponova

и другие.

Genes, Год журнала: 2025, Номер 16(3), С. 296 - 296

Опубликована: Фев. 28, 2025

Members of the Leguminosae family are important crops that provide food, animal feed and vegetable oils. Legumes make a substantial contribution to sustainable agriculture nitrogen cycle through their unique ability fix atmospheric in agricultural ecosystems. Over past three decades, Medicago truncatula Lotus japonicus have emerged as model plants for genomic physiological research legumes. The advancement innovative molecular genetic tools, particularly insertional mutagenesis using retrotransposon Tnt1, has facilitated development extensive mutant collections enabled precise gene tagging identification key symbiotic developmental genes. Building on these resources, twelve years ago, our team initiated establishment platform functional studies legumes Bulgaria. In framework this initiative, we conducted systematic sequencing selected lines identified genes involved plant growth detailed characterization. This review summarizes findings functions species, discusses mechanisms underlying processes examines potential translation fundamental knowledge improve commercially legume Bulgaria globally.

Язык: Английский

Процитировано

0
From root to embryogenic transition: WOX5 reprograms plant somatic cells via auxin-mediated pathways DOI Creative Commons
Anna M. Wójcik,

Kamila Krypczyk,

Weronika M Buchcik

и другие.

BMC Plant Biology, Год журнала: 2025, Номер 25(1)

Опубликована: Май 15, 2025

Язык: Английский

Процитировано

0
A Comparative Transcriptome Analysis Reveals the Molecular Mechanisms That Underlie Somatic Embryogenesis in Peaonia ostii ‘Fengdan’ DOI Open Access

Huiting Ci,

Changyue Li,

Theint Thinzar Aung

и другие.

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(18), С. 10595 - 10595

Опубликована: Сен. 13, 2022

Low propagation rate is the primary problem that limits industry development of tree peony. In this study, a highly efficient regeneration system for peony using somatic embryogenesis (SE) was established. The transcriptomes zygotic embryo explants (S0), non-embryonic callus (S1), embryonic (S2), embryos (S3), and regenerated shoots (S4) were analyzed to determine regulatory mechanisms underlie SE in differentially expressed genes (DEGs) identified pairwise comparisons S1-vs-S2 S1-vs-S3, respectively. enriched DEGs primarily involved hormone signal transduction, stress response nucleus (epigenetic modifications). results indicated cell division, particularly asymmetric enhanced S3. Moreover, implicated fate determination played central roles Hormone pathways work concert with epigenetic modifications responses regulate SE. SERK, WOX9, BBM, FUS3, CUC, WUS characterized as molecular markers To our knowledge, first study transcriptome sequencing. These will improve understanding underly benefit genetic engineering plant.

Язык: Английский

Процитировано

11
The improvement of the in vitro plant regeneration in barley with the epigenetic modifier of histone acetylation, trichostatin A DOI Creative Commons
Katarzyna Nowak, Barbara Wójcikowska, Monika Gajecka

и другие.

Journal of Applied Genetics, Год журнала: 2023, Номер 65(1), С. 13 - 30

Опубликована: Ноя. 14, 2023

Abstract Genotype-limited plant regeneration is one of the main obstacles to broader use genetic transformation in barley breeding. Thus, developing new approaches that might improve responses vitro recalcitrant genotypes remains at center biotechnology. Here, we analyzed different genotypes, including “Golden Promise,” a genotype commonly used transformation, and four malting cultivars poor regenerative potential. The expression hormone-related transcription factor (TF) genes with documented roles was various plant-regenerating capacities. results indicated differential auxin-related TF between both explants derived cultures. In support role auxin regeneration, distinct differences accumulation free oxidized were observed explant-derived callus cultures genotypes. Following assumption modifying gene barley, treated trichostatin A (TSA), which affects histone acetylation. effects TSA genotype-dependent as treatment improved two cultivars. TSA-induced changes associated increased biosynthesis-involved TFs . study demonstrated explant chromatin modifiers such provide effective epigenetic approach improving

Язык: Английский

Процитировано

6