Springer eBooks, Год журнала: 2023, Номер unknown, С. 269 - 288
Опубликована: Янв. 1, 2023
Язык: Английский
Smart Materials in Medicine, Год журнала: 2024, Номер 5(2), С. 183 - 195
Опубликована: Янв. 12, 2024
Since the need for vascular networks to supply oxygen and nutrients while expelling metabolic waste, most cells can only survive within 200 μm of blood vessels; thus, construction well-developed vessel is essential manufacture artificial tissues organs. Three-dimensional (denoted as 3D) printing a scalable, reproducible high-precision manufacturing technology. In past several years, there have been many breakthroughs in building various vascularized tissues, greatly promoting development biological tissue engineering. This paper highlights latest progress 3D printed organs, including heart, liver, lung, kidney, penis. We also discuss application status potential above prospect further requirement technology clinically useable tissues.
Язык: Английский
Процитировано
17
Green Analytical Chemistry, Год журнала: 2024, Номер 8, С. 100096 - 100096
Опубликована: Янв. 13, 2024
The ethical considerations inherent in the use of living organisms research, particularly field ecotoxicology, have prompted a paradigm shift towards more sustainable methodologies. This perspective explores intersection principles and scientific advancements ecotoxicological emphasizing advocacy for non-vertebrate animals, vitro silico alternatives. In methods, which utilize isolated cells, tissues, or organs, approaches, involving computational simulations, present viable alternatives that align with Replacement, Reduction, Refinement (3Rs). Additionally, inclusion animals studies further expands scope considerations. reflects commitment to minimizing environmental impact while advancing understanding. integration these alternative methods not only addresses concerns but also contributes reduction animal testing, allowing precise control over experimental conditions. As technological capabilities validation efforts progress, combined advantages vitro, silico, approaches stand as instrumental components evolution research
Язык: Английский
Процитировано
16
Current topics in developmental biology/Current Topics in Developmental Biology, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
1
Journal of Developmental Biology, Год журнала: 2023, Номер 11(1), С. 14 - 14
Опубликована: Март 18, 2023
Nephrons are the functional units which comprise kidney. Each nephron contains a number of physiologically unique populations specialized epithelial cells that organized into discrete domains known as segments. The principles segment development have been subject many studies in recent years. Understanding mechanisms nephrogenesis has enormous potential to expand our knowledge about basis congenital anomalies kidney and urinary tract (CAKUT), contribute ongoing regenerative medicine efforts aimed at identifying renal repair generating replacement tissue. study zebrafish embryonic kidney, or pronephros, provides opportunities identify genes signaling pathways control development. Here, we describe advances patterning differentiation zebrafish, with focus on distal formation.
Язык: Английский
Процитировано
18
Stem Cell Research & Therapy, Год журнала: 2024, Номер 15(1)
Опубликована: Май 3, 2024
Induced pluripotent stem cells (iPSCs)-derived kidney organoids are a promising model for studying disease mechanisms and renal development. Despite several protocols having been developed, further improvements needed to overcome existing limitations enable wider application of this model. One the approaches improve differentiation in vitro is include system cell types important organogenesis vivo, such as macrophages. Another approach could be survival. Mesodermal lineage common initial step reported protocols. The glycogen synthase kinase-3 (GSK-3) activity inhibitor, CHIR99021 (CHIR), applied induce mesodermal differentiation. It has that CHIR simultaneously induces iPSCs apoptosis can compromise We thought interfere with CHIR-induced using rapamycin. Differentiation from human was performed. Cell survival autophagy were analyzed counting kit 8 (CCK8) Autophagy detection kit. Cells treated rapamycin or co-cultured monocytes isolated peripheral blood iPSCs-macrophages transwell co-culture system. Monocyte-derived extracellular vesicles (EVs) polyethylene glycol precipitation. Expression apoptotic markers cleaved Caspase 3, Poly [ADP-ribose] polymerase 1 (PARP-1) T-Box Transcription Factor 6 (TBX6), odd-skipped related (OSR1), Nephrin, E-Cadherin, Paired box gene 2 (Pax2) GATA Binding Protein 3 (Gata3) assessed by RT-PCR western blotting. Organoids imaged 3D-confocal microscopy. observed induced during stage organoid Underlying implied accumulation reactive oxygen species decreased autophagy. Activation rapamacin an indirect differentiating prevented CHIR. Furthermore, (but not rapamycin) strongly promoted expression development via released vesicles. Our data suggest co-culturing improves organoids. An underlying mechanism monocytic action implies, but limited to, increased CHIR-treated iPSCs. findings enhance utility models.
Язык: Английский
Процитировано
5
SLAS DISCOVERY, Год журнала: 2024, Номер 29(3), С. 100141 - 100141
Опубликована: Янв. 11, 2024
High Throughput Screening (HTS) with 3D cell models is possible thanks to the recent progress and development in culture technologies. Results from multiple studies have demonstrated different drug responses between 2D culture. It now widely accepted that more accurately represent physiologic conditions of tumors over models. However, there still a need for accurate tests are scalable better imitate complex living tissues. Here, we describe ultrahigh throughput methods response profiling patient derived primary including melanoma as well renal carcinoma were tested against NCI oncologic set FDA approved drugs. We also their autologous cancer associated fibroblasts, varied in-vitro using matrix vs free completed this both rendered cells. The result indicates heterologous drugs based on genetic background, but not maintenance condition. present supporting results HTS efforts these organoids patients. This possibility cells expands our screening capabilities testing other types clinically anti-cancer agents find potential off label use.
Язык: Английский
Процитировано
4
Cells, Год журнала: 2024, Номер 13(14), С. 1190 - 1190
Опубликована: Июль 13, 2024
Genetic or hereditary kidney disease stands as a pivotal cause of chronic (CKD). The proliferation and widespread utilization DNA testing in clinical settings have notably eased the diagnosis genetic diseases, which were once elusive but are now increasingly identified cases previously deemed CKD unknown etiology. However, despite these diagnostic strides, research into pathogenesis novel drug development faces significant hurdles, chiefly due to dearth appropriate animal models challenges posed by limited patient cohorts studies. Conversely, advent human-induced pluripotent stem cells (hiPSCs) offer promising avenue for research. Particularly, hiPSC-derived organoid systems presents platform investigating various forms diseases. Moreover, integration CRISPR/Cas9 technique this system holds immense potential efficient on This review aims explore applications vitro organoids generated from hiPSCs study diverse Additionally, it will delve limitations outline future perspectives advancing crucial area.
Язык: Английский
Процитировано
4
EFSA Supporting Publications, Год журнала: 2024, Номер 21(9)
Опубликована: Сен. 1, 2024
Abstract New Approach Methodologies (NAMs), broadly understood to include in silico, chemico, vitro and ex vivo methods, show great potential advancing risk assessment albeit their regulatory implementation is lagging. The EFSA Guidance on of nanomaterials (EFSA Nano‐RA) suggests nano‐specific best achieved through Integrated Approaches Testing Assessment (IATAs) with NAMs as the first choice generate new information. Integrating promises several advantages such a better human focus, more detailed insights into molecular mechanisms higher efficacy. However, applying NMs also poses considerable challenges issues related dispersion stability, dosimetry, agglomeration, dissolution, transformations or assay interferences. Significant efforts are being undertaken by standardisation organisations research projects establish various for NMs. Here thorough review provided covering that will be potentially useful food feed sector. It follows structure Nano‐RA expands it, where needed, support decision‐making selection NM assessment. begins an overview NAM‐frameworks, followed description individual including those relevant physicochemical characterisation, exposure hazard toxicodynamics toxicokinetics. focus concerning degradation/dissolution, genotoxicity, cytotoxicity, oxidative stress, (pro‐)inflammation, barrier integrity important endpoints initial screening according framework. As result, total 267 nano‐relevant NAMs, mostly “not validated” (with few notable exceptions), were included this review. Validation notwithstanding, could already prove reliable NMs, especially integrated approaches.
Язык: Английский
Процитировано
4
Frontiers in Cellular and Infection Microbiology, Год журнала: 2025, Номер 15
Опубликована: Фев. 18, 2025
Despite decades of control efforts, the prevalence schistosomiasis remains high in many endemic regions, posing significant challenges to global health. One key factors contributing persistence disease is complex life cycle Schistosoma parasite, causative agent, which involves multiple stages development and intricate interactions with its mammalian hosts snails. Among various parasite lifecycle, deposition eggs their migration through host tissues significant, as they initiate onset pathology by inducing inflammatory reactions tissue damage. However, our understanding mechanisms underlying egg extravasation limited, hindering efforts develop effective interventions. Microphysiological systems, particularly organ-on-a-chip offer a promising approach study this phenomenon controlled experimental setting because allow replication physiological microenvironments vitro. This review provides an overview schistosomiasis, introduces concept technology, discusses potential applications field research.
Язык: Английский
Процитировано
0
Materials Today Bio, Год журнала: 2025, Номер 31, С. 101600 - 101600
Опубликована: Фев. 21, 2025
Recent advancements in tissue engineering offer promising solutions for the repair and reconstruction of urinary system, particularly cases organ injuries. Historically, autologous grafts allografts have been primary options repairing damaged tissues. However, these approaches often lead to complications such as immune rejection, donor site morbidity, functional limitations. Tissue provides a more sustainable alternative by leveraging potential cells, bioactive scaffolds, growth factors regenerate This approach aims not only restore structure but also enhance recovery. Key challenges field include achieving adequate vascularization, overcoming responses, ensuring long-term integration. innovations, 3D bioprinting, stem cell-based therapies, development novel biomaterials, show great promise addressing challenges. review explores current state applications urological focusing on regeneration bladder, urethra, kidneys. We discuss recent breakthroughs, ongoing clinical trials, emerging technologies, well improve outcomes. Finally, we outline critical future directions urology, emphasizing need interdisciplinary collaboration overcome existing barriers accelerate translation.
Язык: Английский
Процитировано
0