Russian Journal of Human Reproduction, Год журнала: 2023, Номер 29(4), С. 29 - 29
Опубликована: Янв. 1, 2023
Язык: Английский
Journal of Obstetrics and Gynaecology Research, Год журнала: 2025, Номер 51(1)
Опубликована: Янв. 1, 2025
Abstract Aims Aberrantly expressed MPHOSPH9 has been reported to be associated with poor prognosis in many diseases. Previous study indicates that is abnormally patients uterine fibroids (UFs). This focused on the possible prognostic value of UFs after high intensity ultrasound (HIFU) treatment. Methods A total 455 participated study, including 95 who needed reintervention (Reintervention group) and 360 did not need (Non‐reintervention HIFU They volunteered blood samples before The relative expression was assessed by qRT‐PCR. Cox regression analysis performed assess risk factors postoperative reintervention. Results markedly elevated Reintervention, compared Non‐reintervention group ( p < 0.001). Five‐year follow‐up results showed among these patients, required time mainly occurred during 10–40 months surgery. demonstrated size (hazard ratio = 2.769, 95% confidence interval: 1.366–5.610) level 3.160, 1.506–6.630) were independent for Conclusions enhanced a potential candidate predicting patients.
Язык: Английский
Процитировано
0
Cell Proliferation, Год журнала: 2025, Номер unknown
Опубликована: Март 20, 2025
ABSTRACT Uterine fibroids (UFs) are the most common benign gynecologic tumours affecting women of reproductive age. This study aims to deepen understanding UFs complex aetiology through harnessing power 3D organoid models derived from human myometrial stem cells emulate in vivo behaviour these tumours. Isolated SCs were cultured over 7 days under a defined culture system. Immunohistochemistry, Immunofluorescence, stiffness, RNA Sequencing was conducted, and differential gene expression assessed using RT‐PCR. The organoids exhibited diverse populations cells, including smooth muscle, fibroblasts. Excessive ECM deposition shown via Collagen Fibronectin expression. We confirmed that our expressed oestrogen receptor pattern similar their corresponding tissue, as well responded steroid hormone. Interestingly, we revealed significant racial disparities accumulation within different groups. augmented is theorised enhance tissue Young's modulus. Additionally, research demonstrated decreases fibrotic markers upon treatment with Vitamin D3 Doxercalciferol. Furthermore, pro‐fibroid effects environmental phthalates further elucidate potential factors contributing UF pathology. model can serve robust platform underlying molecular mechanisms UFs, besides offering invaluable insights for therapeutic interventions.
Язык: Английский
Процитировано
0
Diagnostics, Год журнала: 2024, Номер 14(18), С. 2046 - 2046
Опубликована: Сен. 14, 2024
Uterine leiomyomas are the most common benign uterine tumors in women and often asymptomatic, with clinical manifestation occurring 20–25% of cases. The diagnostic pathway begins suspicion includes an ultrasound examination, hysteroscopy, and, when deemed necessary, magnetic resonance imaging. decision-making process should consider impairment quality life due to symptoms, reproductive desire, malignancy, course, woman’s preferences. Despite absence a definitive cure, management fibroid-related symptoms can benefit from various medical therapies, ranging symptomatic treatments latest hormonal drugs aimed at reducing impact fibroids on women’s well-being. When therapy is not solution for patient, it be used as bridge prepare patient surgery. Surgical approaches continue play crucial role treatment fibroids, gynecologist has opportunity choose surgical options tailor intervention patient’s needs. This review aims summarize necessary assessment fibromatosis, presenting all available address needs different types women.
Язык: Английский
Процитировано
4
European Journal of Pharmacology, Год журнала: 2025, Номер 996, С. 177439 - 177439
Опубликована: Март 3, 2025
Язык: Английский
Процитировано
0
Cell and Organ Transplantology, Год журнала: 2024, Номер 12(1)
Опубликована: Май 1, 2024
Uterine leiomyoma is one of the most common gynaecological diseases, which often leads to loss fertility. It known that in this pathology, damage tissues uterus accompanied by oxidative stress, and mechanisms its compensation play a decisive role process myometrial regeneration, especially when performing organ-preserving operations. The aim study was determine levels melatonin, reproductive hormones state angiogenesis, antioxidant system lipid peroxidation women age who were diagnosed with uterine leiomyoma. Material methods. 60 (study group) examined. control group consisted 20 healthy same group. Melatonin women's blood determined once, on an empty stomach, at 8 a.m.; concentrations estradiol progesterone follicular phase menstrual cycle also measured. angiogenesis studied examining vascular endothelial growth factor (VEGF) content final metabolites nitric oxide NO plasma. activity assessed plasma reduced glutathione, glutathione peroxidase, transferase. Indicators investigated malondialdehyde (MDA) erythrocytes. Results. found patients leiomyoma, there significantly lower level melatonin (111.1 ± 18.5 ng/mL) compared (153.5 8.5 ng/mL), while concentration almost three times higher (107.4 25.3 pg/mL) (36.2 3.2 pg/mL), 1.9 (2.1 0.4 ng/mL 1.1 0.5 group). VEGF (90.4 23.6 (35.1 8.3 as well oxide, reached 5.9 pg/mL 9.9 3.9 (0.77 0.13 µmol/L) (1.02 0.14 group, glutathione-S-transferase peroxidase enzymes (161.3 22.3 235.7 35.9 ng/mL, respectively), whereas these indicators 118.9 18.0 105.3 41.2 respectively. MDA higher, measuring 5.2 0.8 nmol/L 10.8 erythrocytes 2.3 5.3 Conclusions. lower, estradiol, progesterone, glutathione-S-transferase, VEGF, average erythrocytes, women. In relationship between described using linear regression equation, which, we suggest, indicates activating effect stress myometrium. our opinion, occurs due inadequate system, integral part leading enhanced against background estrogen-induced proliferation.
Язык: Английский
Процитировано
2
International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(17), С. 13441 - 13441
Опубликована: Авг. 30, 2023
The objective of this study was to determine if the aberrant expression select genes could form basis for racial disparity in fibroid characteristics. next-generation RNA sequencing results were analyzed as fold change [leiomyomas/paired myometrium, also known differential (DF)], comparing specimens from White (n = 7) and Black 12) patients. analysis indicated that 95 minimally changed tumors (DF ≈ 1) but significantly altered by more than 1.5-fold (up or down) Twenty-one novel selected confirmation 69 paired fibroids qRT-PCR. Among these 21, coding transcripts FRAT2, SOX4, TNFRSF19, ACP7, GRIP1, IRS4, PLEKHG4B, PGR, COL24A1, KRT17, MMP17, SLN, CCDC177, FUT2, MYO5B, MYOG, ZNF703, CDC25A, CDCA7 higher, while DAB2 CAV2 lower Hispanic patients compared with Western blot revealed a greater PGR-A total progesterone (PGR-A PGR-B) Collectively, we identified set uniquely expressed race/ethnicity-dependent manner, which underlying mechanisms their associated symptoms.
Язык: Английский
Процитировано
6
Drugs, Год журнала: 2023, Номер 83(18), С. 1649 - 1675
Опубликована: Ноя. 3, 2023
Язык: Английский
Процитировано
6
International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(21), С. 15972 - 15972
Опубликована: Ноя. 4, 2023
Uterine fibroids (UFs) are common tumors in women of reproductive age. It is imperative to comprehend UFs’ associated risk factors facilitate early detection and prevention. Simple relying on surgical/pharmacological treatment advanced disease not only highly expensive, but it also deprives patients good quality life (QOL). Unfortunately, even if the discovered early, no medical intervention traditionally initiated until burden becomes high, then surgical performed. Furthermore, after myomectomy, recurrence rate UFs extremely high with need for additional surgeries other interventions. This confused approach invasive costly an overall negative impact women’s health. Secondary prevention management slow down its progression or halt completely. The current watchful observation considered a major missed opportunity literature. aim this article present named ESCAPE (Evidence-Based Approach Prevention) UF management. comprises simple, inexpensive, safe steps that can arrest development UFs, promote health, decrease number unnecessary surgeries, save billions health care systems’ dollars worldwide.
Язык: Английский
Процитировано
6
The FASEB Journal, Год журнала: 2024, Номер 38(21)
Опубликована: Окт. 30, 2024
Abstract The role of long non‐coding RNAs in fibroid pathogenesis remains largely unexplored. In a previous study, we found elevated XIST (X‐inactive specific transcript) levels fibroids, which sponged miR‐29c and miR‐200c, leading to the overexpression their target genes. This study aimed assess therapeutic potential downregulation treatment. Ovariectomized SCID (severe combined immunodeficiency) mice were implanted with tumors transduced siRNA or control via lentivirus. After 1 month, animals sacrificed xenografts removed for further analysis. knockdown reduced tumor weight by 15% increased miR‐200c expression 3.9‐fold 2.2‐fold, respectively. mRNA targets ( COL3A1 , TGF‐β3 CDK2 SPARC ) FN1 TDO2 ), as well PRL E2F1 EZH2 was significantly decreased. Protein abundance collagen, COL3A1, FN1, CDK2, SPARC, also reduced. IHC analysis xenograft sections using markers Ki67 cell proliferation cleaved caspase 3 apoptosis showed decreased no changes xenografts. revealed collagen staining nuclei These results indicate that fibroids has beneficial effects, reducing growth genes involved proliferation, inflammation, extracellular matrix regulation.
Язык: Английский
Процитировано
1
European Journal of Obstetrics & Gynecology and Reproductive Biology, Год журнала: 2024, Номер 303, С. 302 - 309
Опубликована: Ноя. 1, 2024
Язык: Английский
Процитировано
1